Phylogenetic analysis of MLV sequences from longitudinally sampled Chronic Fatigue Syndrome patients suggests PCR contamination rather than viral evolution. Abstract:
This is an analysis of sequences from the Lo study.
http://jvi.asm.org/cgi/content/abstract/JVI.00827-11v1
Xenotropic murine leukemia virus (XMRV) has been amplified from human prostate cancer and chronic fatigue syndrome (CFS) patient samples. Other studies failed to replicate these findings and suggested PCR contamination with a prostate cancer cell line, 22Rv1, as a likely source. MLV-like sequences have also been detected in CFS patients in longitudinal samples 15 years apart. Here we test whether sequence data from these samples are consistent with viral evolution. Our phylogenetic analyses strongly reject a model of within-patient evolution and demonstrate that the sequences from the first and second time points represent distinct endogenous murine retroviruses suggesting contamination.
This is an analysis of sequences from the Lo study.
http://jvi.asm.org/cgi/content/abstract/JVI.00827-11v1