I worked up to 400mcg methylfolate and - wow! - that stuff surely had an effect on me. More energy, more neurotransmitters, less need for thyroid glandular and ribose, and unfortunately - I have nightly insomnia & intermittent anxiety. I felt wired and unable to sleep - not just tossing and turning - but once incident where I went to the gym and pumped iron at 3 a.m.... and once or twice where I wanted to go out to a club (!) after midnight. After working for years on my sleep and finally able to sleep like a baby (before methylation) - this is disruptive and distressing for work and my marginal vapors of a "social life." Do you believe these symptoms are "startup" effects that need to be endured through at the same doses.. and will calm down? Or do you consider this "Overmethylation" where you need to take some niacin... and back the dose down? This is what Dr. Lynch seems to say. He supplements are supplemental.. and you should only take them when you need to supplement.. not necessarily every day on a schedule.