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No evidence of cross-species transmission of mouse retroviruses to animal workers

Discussion in 'XMRV Research and Replication Studies' started by Firestormm, Jan 4, 2012.

  1. Firestormm

    Firestormm Guest

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    Cornwall England
    No evidence of cross-species transmission of mouse retroviruses to animal workers exposed to mice

    James Brooks,
    Karly Lycett-Lambert,
    Kyna Caminiti,
    Harriet Merks,
    Rachel McMillan,
    Paul Sandstrom

    Article first published online: 30 DEC 2011:http://onlinelibrary.wiley.com/doi/10.1111/j.1537-2995.2011.03463.x/abstract

    BACKGROUND:
    Although recent data have brought into question the association between xenotropic murine leukemia virus-related virus (XMRV) and chronic fatigue syndrome, one group has reported evidence of human infection with distinct polytropic murine leukemia viruses (MLVs). Occult retroviral infection among humans poses a significant public health risk should it be introduced into the blood supply. To explore the possibility of cross-species transmission of MLVs to humans, we sought molecular and serologic evidence of XRMV/MLV infection among a cohort of animal workers highly exposed to mice.

    STUDY DESIGN AND METHODS:
    Before the commencement of the study, the laboratory and equipment were demonstrated to be free of XMRV/MLV DNA sequences. DNA extracted from 43 animal workers was tested using nested polymerase chain reaction (PCR) with published primer sets, targeting regions of XMRV and MLV gag. Negative controls were assayed in a 1:1 ratio with specimens. Serum specimens were tested using a validated immunoblot assay containing cross-reactive XMRV antigens.

    RESULTS:
    Initial molecular assays demonstrated that the physical space and laboratory equipment were free of MLV and XMRV DNA sequences. Nested PCR assays using multiple primer sets successfully amplified XMRV and MLV sequences from positive controls with high sensitivity. A single, nonreproducible, false-positive result from one specimen was shown to be the result of subsequent contamination. Immunoblotting of all subjects' sera failed to demonstrate any evidence of seroreactivity to XMRV proteins.

    CONCLUSIONS:
    There was no evidence of human infection with XMRV/MLV among a cohort of individuals highly exposed to mice. These data suggest that the likelihood of cross-species transmission events of MLV from mice to humans is low.
     
  2. Jemal

    Jemal Senior Member

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    Thanks for sharing Firestormm.
     
  3. Firestormm

    Firestormm Guest

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    That's all right. Be nice (again) to see the full paper but I see you have reposted on the other forum now so perhaps a 'boosh' will result.
     
  4. caledonia

    caledonia

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    I didn't know animals worked in labs -;) lol. Sorry couldn't resist.
     
  5. justinreilly

    justinreilly Stop the IoM & P2P! Adopt CCC!

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    NYC (& RI)
    The chance that one individual working for a few months with mice under proper conditions gets MLVs seems to be very low. We don't know what the chance is that a substantial absolute number (not percentage) of lab workers have been infected.
     

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