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New Test Distinguishes Viral vs Bacterial Infections

Discussion in 'Other Health News and Research' started by Gemini, Sep 20, 2013.

  1. Gemini

    Gemini Senior Member

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  2. aimossy

    aimossy Senior Member

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    sorry Gemini it required a password to open, sounds interesting. maybe its just my computer that requires the password, im also technologically challenged.:)
     
    rosie26 likes this.
  3. Ema

    Ema Senior Member

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    Midwest USA
    Here's the abstract:

    Abstract


    Improved ways to diagnose acute respiratory viral infections could decrease inappropriate antibacterial use and serve as a vital triage mechanism in the event of a potential viral pandemic. Measurement of the host response to infection is an alternative to pathogen-based diagnostic testing and may improve diagnostic accuracy. We have developed a host-based assay with a reverse transcription polymerase chain reaction (RT-PCR) TaqMan low-density array (TLDA) platform for classifying respiratory viral infection. We developed the assay using two cohorts experimentally infected with influenza A H3N2/Wisconsin or influenza A H1N1/Brisbane, and validated the assay in a sample of adults presenting to the emergency department with fever (n = 102) and in healthy volunteers (n = 41). Peripheral blood RNA samples were obtained from individuals who underwent experimental viral challenge or who presented to the emergency department and had microbiologically proven viral respiratory infection or systemic bacterial infection. The selected gene set on the RT-PCR TLDA assay classified participants with experimentally induced influenza H3N2 and H1N1 infection with 100 and 87% accuracy, respectively. We validated this host gene expression signature in a cohort of 102 individuals arriving at the emergency department. The sensitivity of the RT-PCR test was 89% [95% confidence interval (CI), 72 to 98%], and the specificity was 94% (95% CI, 86 to 99%). These results show that RT-PCR–based detection of a host gene expression signature can classify individuals with respiratory viral infection and sets the stage for prospective evaluation of this diagnostic approach in a clinical setting.

    • Copyright © 2013, American Association for the Advancement of Science
    Citation: A. K. Zaas, T. Burke, M. Chen, M. McClain, B. Nicholson, T. Veldman, E. L. Tsalik, V. Fowler, E. P. Rivers, R. Otero, S. F. Kingsmore, D. Voora, J. Lucas, A. O. Hero, L. Carin, C. W. Woods, G. S. Ginsburg, A Host-Based RT-PCR Gene Expression Signature to Identify Acute Respiratory Viral Infection. Sci. Transl. Med. 5, 203ra126 (2013).
    http://stm.sciencemag.org/content/5/203/203ra126
     
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  4. aimossy

    aimossy Senior Member

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    thanks, wow 10 years from now tests from gps are going to be soo different. gene expression and faecal tests. :angel:
     
    rosie26 likes this.
  5. Gemini

    Gemini Senior Member

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    Thanks, aimossy, for letting me know. A Medscape site, I didn't realize not everyone can access it .

    I've edited the post adding the PubMed abstract and note.
     
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  6. aimossy

    aimossy Senior Member

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    there is new interesting research out in ploSONE journal and medic express sites on chrons disease,considering focus on gut I thought would be good to put up link to it but im hopeless with doing it. its about chrons lacking an enzyme that helps clear bad bacteria.
     

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