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new publication from dong % silverman

Discussion in 'XMRV Research and Replication Studies' started by Eric Johnson from I&I, Nov 13, 2009.

  1. Eric Johnson from I&I

    Eric Johnson from I&I Senior Member

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    J Virol. 2009 Nov 11. [Epub ahead of print]
    Androgen Stimulates Transcription and Replication of XMRV (Xenotropic Murine Leukemia Virus-Related Virus).

    Dong B, Silverman RH.

    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195.

    XMRV is a gammaretrovirus originally identified in a subset of prostate cancer patients. Because androgens stimulate prostate tumors and some retroviruses, we investigated effects of dihydrotestosterone (DHT) on XMRV transcription and replication. Transcription from the XMRV U3 region was stimulated up to 2-fold by DHT, but only in cells containing a functional androgen receptor. Mutations in the glucocorticoid response element (GRE) of XMRV impaired basal transcription and androgen responsiveness. Furthermore, DHT stimulated XMRV replication by 3-fold, whereas androgen inhibitors (casodex and flutamide) suppressed viral growth up to 3-fold. Findings suggest that integration of the XMRV LTR into host DNA could impart androgen stimulation on cellular genes.

    PMID: 19906923 [PubMed - as supplied by publisher]
  2. caledonia

    caledonia

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    Cincinnati, OH, USA
    Interesting - it sounds like androgen inhibitors could be a treatment. Would that only be in males?
  3. _Kim_

    _Kim_ Guest

    Excerpts from full text

    Note: WPI study is cited in the 2nd sentence of the article!!

  4. Alice Band

    Alice Band PWME - ME by Ramsay

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    UK
    I wonder if this explains why women with PCOS & CFS seem to go together in some women

    <snip from PCOS description>

    Another cardinal feature of PCOS is increased androgen (male hormone) production. Therefore, women with PCOS almost always have facial hirsutism (excessive hair) and frequently have acne as well.
  5. Advocate

    Advocate Senior Member

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    U.S.A.
    Hi Kim,

    I couldn't find the full text anywhere. Where did you get it? Did they cite the Mikovits article in the references at the end?


    Hi Eric,

    It would be wonderful if you (or anyone) would explain in detail how they did this in the lab.
  6. shrewsbury

    shrewsbury member

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    Hi Advocate. Here's the link

    Androgen Stimulates Transcription and Replication of XMRV (Xenotropic Murine Leukemia Virus-Related Virus).
    Dong B, Silverman RH.

    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195
  7. _Kim_

    _Kim_ Guest

    Full text by subscription

    Hi Advocate, I have library privileges through my university that allows me to access many full text articles. This was one of them. The link that islandfinn posted only brings me to the abstract that Eric posted in the first message above.

    Yes. Below is the reference. This would make it the first time the article was cited in another paper. Exciting!


    The details are in the article. I'll pull the article back up again and post some of these another post
  8. _Kim_

    _Kim_ Guest

    Lab Procedures

    Aftermath? I hope quoting like this doesn't violate copyrights. Let me know if I need to modify/delete these.

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