My SNP Results. Would appreciate your comments.

Hi there @caledonia, @Valentijn, @Sea, @Critterina and any other SNP gurus on PR. Appreciate your comments on my methylation results.

My blood tests show consistently high folate and mid range B12. I am currently only supplementing with a multivitamin. I am on metformin for skinny type 2 diabetes and apparently this can negatively affect B12 levels.

MAO-A R297R +/+ rs6323 TT
MTHFR C677T +/+ rs1801133 AA
MTRR K350A +/+ rs162036 GG

COMT V158M +/- rs4680 AG
COMT H62H +/- rs4633 CT
VDR Bsm +/- rs1544410 CT
VDR Taq +/- rs731236 AG
ACAT1-02 +/- rs3741049 AG
BHMT-02 +/- rs567754 CT
BHMT-08 +/- rs651852 CT
AHCY-01 +/- rs819147 CT
AHCY-19 +/- rs819171 CT
CBS A360A +/- rs1801181 CT

COMT P199P -/- rs769224 GG
MTHFR 03 P39P -/- rs2066470 GG
MTHFR A1298C -/- rs1801131 TT
MTR A2756G -/- rs18005087 AA
MTRR A66G -/- rs1801394 AA
MTRR A664A -/- rs1802059 GG
CBS C699T -/- rs234706 GG

Also had 6 "no calls". Are they relevant?

Sorry brain not working well today and I accidentally posted this before keying in the SNPs

 

Hi there @caledonia, @Valentijn, @Sea, @Critterina and any other genetic gurus on PR. Appreciate your comments on my detox results. I have lifelong sensitivities to various medications, chemicals etc which are much worse since ME. Also now have food allergies and intolerances.

CYP1B1 L432V +/+ rs1056836 GG
CYP1B1 N453S +/+ rs1800440 CC

CYP1B1 R48G +/- rs10012 CG
CYP2C9*3 A1075C +/- rs1057910 AC
CYP2C19*17 +/- rs12248560 CT
CYP2D6 S486T +/- rs1135840 CG
CYP2D6 100C>T +/- rs1065852 AG
SOD2 A16V +/- rs4880 AG
NAT2 I114T +/- rs1801280 CT
NAT2 G286E +/- rs1799931 AG
NAT2 K268R +/- rs1208 AG

CYP1A1*2C A4889G -/- rs1048943 TT
CYP1A1 m3 T3205C -/- rs4986883 TT
CYP1A1 C2453A -/- rs1799814 GG
CYP1A2 164A>C -/- rs762551 AA
CYP2A6*2 1799T>A -/- rs1801272 AA
CYP2A6*20 -/- rs28399444 II
CYP2C9*2 C430T -/- rs1799853 CC

CYP2D6 2850C>T -/- rs16947 GG
CYP2E1*1B 9896C>G -/- rs2070676 CC
CYP2E1*1B 10023G>A -/- rs55897648 GG
CYP2E1*4 4768G>A -/- rs6413419 GG
CYP3A4*1B -/- rs2740574 TT
CYP3A4*2 S222P -/- rs55785340 AA
CYP3A4*3 M445T -/- rs4986910 AA
CYP3A4*16 T185S -/- rs12721627 GG
GSTP1 I105V -/- rs1695 AA
GSTP1 A114V -/- rs1138272 CC

NAT1 R187Q -/- rs4986782 GG
NAT1 R64W -/- rs1805158 CC
NAT2 R197Q -/- rs1799930 GG
NAT2 R64Q -/- rs1801279 GG

GSTT1 present

Many thanks Andy

 

@AndyPandy ,

I love your username! Sweet!

Well, I don't think I have a lot to add to the conversation, even though it's just beginning. The really important one is your MTHFR C677T +/+ and you are probably already taking methylfolate for that. I'm not sure how accurate the blood levels of folate are, and I hope that someone who knows will chime in.

I do know that blood levels of B12 do not reflect the levels of methylB12 that is the active form. Although you don't have any of the SNPs that would affect your need for the active methyl form of B12, it's possible that the metformin could mess with the activation process. Again, ONLY speculation, to consider if you feel you have B12 issues (peripheral neuropathy is a big indicator, and I understand they can tell in a diabetic what neuropathy is diabetic and what is not).

I'm really getting into the histamine intolerance lifestyle (although I would love to leave it) because it makes me able to live, and not spaced out on all those breathing meds. If you react to tomatoes, spinach, cottage cheese, and cinnamon, chat me up. Those were my first clues. I know next to nothing about other intolerances, although I read a sulfite intolerance book along my journey and may be able to find it again online if you need it.

Best wishes!

Crit

 

Hi @Critterina

Thanks so much for responding.

I am totally new to the world of methylation and I am not currently taking any specific supplements for it. I am just trying to find my way and wondering about whether to try B12 supplementation. My folate intake is from food and a standard multivitamin.

I do have neuropathy, but neither the endocrinologist nor the neurologist can pinpoint the cause. It started in my right little toe in around 2007 and has spread to my other toes and the ball of my foot. It started in my right little finger in around 2012 and has spread to the ring finger. Only on my right side. I was diagnosed with diabetes in 1997 but may have had it earlier. I started taking metformin in 2011. May not be relevant but I had shingles in 1981.

I am interested in learning more about histamine intolerance. I suspect this might be relevant to me but it is not an area I have looked into yet. Can you recommend a source of useful information for this?

I am sulphite intolerant and have a gazillion other food allergies and intolerances .

Best wishes to you

Andy

 

@AndyPandy Just curious, did you have neuropathy before or after you took Statins? I will have my 23andMe results back in about 3 wks and will be asking you guys for help as well! It is great that we can all figure this out together.

 

I took the statins in 2011. I don't think they relate to the neuropathy. I have just discovered I have a number of genes which put me at higher risk of developing myopathy from taking statins. Also genes which increase the effects of statins.

 

If you are not taking methylfolate (for the MTHFR C677T) then your high folate levels are a good indication that you are not processing and using the folic acid that you would be getting from your diet. All our bread, cereals and wheat flours are now fortified with folic acid which is a real problem for those with a defective MTHFR gene. The high levels means it's in your blood, but probably not being converted and used too well.

You would do well to avoid folic acid as far as possible and instead supplement with methylfolate which is the active already converted folate our bodies need.

Seeing as your B12 is mid-range it would be wise to supplement with MethylB12 as well because methylfolate and B12 work together and there can be some danger to taking methylfolate without adequate levels of B12.

 

SNPedia and dbSNP are both good resources for checking out frequency and effects of various snps.

For example your first detox snp:

http://www.snpedia.com/index.php/Rs1056836

http://www.ncbi.nlm.nih.gov/SNP/snp_ref.cgi?rs=1056836

They also have links to further research eg

http://omim.org/entry/601771

A no call usually means it couldn't be determined what allele you had at that particular snp. It's a technical issue with not enough DNA in the sample at that point and everyone gets some no calls on their results.

Sometimes it can mean you have a deletion at that point but there are less of those.

 

Hi @Sea

Thanks so much for responding.

I am very sensitive to medications, chemicals etc. I understand the start low go slow concept, but I'm wondering is MethylB12 generally well tolerated or is there an alternative form of B12 that might be better? Are there any brands you can suggest for B12 and methyl folate? I am in Australia

Best wishes Andy

 

@Sea

Thanks for the research tips!

Best wishes Andy

 

I am in Australia too. I buy most of my supplements online from iherb. Prices are much cheaper than Australia, shipping is usually $8.00 and they take about a week to deliver.

Methyl B12 is usually recommended as the active version, the theory is that our bodies can utilise it more efficiently than the inactive versions. Those who have no trouble converting often use Hydroxycobalamin instead. Some even find they can utilise the Cyanocobalamin - the one found in all the cheap supermarket and chemist supplements.

Personally I don't tolerate Cyanocobalamin. I get an initial lift in energy for 2-3 days then feel dreadful. I do have some snps that are are not normal in the B12 transport genes. I haven't really given Hydroxy much of a trial, I use the MethylB12 and tolerate it well. I use the brand recommended here the most, Enzymatic therapy - it's a sublingual tablet so that maximises the amount of B12 absorbed.

Methyfolate - I use Solgar Folate (800mcg as Metafolin). It comes in smaller doses as well. It is the cheapest genuine methylfolate I have found.

 

My specialist who I was seeing for the mutation you have too with the MTHFR mutation, got me to get my supplements from iherb as its cheap.

You have the worst type of MTHFR mutation. Means you are down about 70% for your folate as your body cant convert it to the active form (ordinary folate is no good for you!). My specialist for this has me on high potency Methycobalamin by Natural factors for this along with a bioactive form of folate, called "MegaFolinic by Source Naturals (my specialist was particular about what brands I brought as he said some seemed to work better then others).

There are also quite a few doctors out there who have an interest in trying to help those who have MTHFR mutation, you could try to seek out someone in your area by doing a search of MTHFR , dr and your city.
..............................

I suggest for you to check out the MTHFR website to learn more about this from the MTHFR expert. http://mthfr.net/ (I notice that Dr Ben Lynch says the following to someone with this mutation .

Treating my MTHFR (my specialist said one cant expect fast improvement as after all your body has done the wrong thing all its life with this) helped my stamina a bit (but it took time but Im sure it was those supplements which helped). I probably would have more improvement with it if I wasnt having to over do it all the time.

 

If you put your 23andME raw data download throu geneticgenie website, you should get a report telling what to do some on your methylation issues , unless they've changed things (this report comes up under the results there

..............................

Ive stole this below info off of someones Genovations gene report for you.

CYP1B1 Health Implications:
Cytochrome P450 1B1 converts estrogen into 1-hydroxy estrogen which may contribute to estrogen-sensitive carinogenicy. CYP1B1 is also partially responsible for detoxifying polycyclic aromatic hydrocarbons (PAHs), the products of the burning of organic materials, like car exhaust, cigarette smoke and charbroiled foods.

(I saw a breast cancer org not long ago warning about this gene and said to not eat charred or burnt meats due to increasing the breast cancer risk in ones who have this).

Minimizing Risk: You should make soy foods (tofu, tempeh and soy beans) and cold water fish (salmon, mackerel etc) a regular part of your diet. Do not smoke. Avoid charbroiled and well done meats. Minimize exposure to car and diesel exhaust. Avoid contact with industrial solvents. Moderate red wine.

Increase the 2-hydroxylation of estrogens:
I3C or dilndolylmethane
Fish oil (EPA/DHA)

Down regulate CYP1B1 induction and expression
DHEA
Hesperetin (a flavonoid)
A daily glass of red wine may be beneficial

 

Thanks @taniaaust1 and @Sea for your valuable advice. Much appreciated.

 

Andy,

I'm attaching what I consider is the definitive article on histamine intolerance and my list of foods (which I also have in German, if you want it.) The "Seems OK" foods are those that I can tolerate in small quantities (a couple of strawberries or blueberries in a fruit salad won't do me in, as peaches will).

You might also check the diamine oxidase (DAO) SNPs in your results. These are the rs numbers and the DAO risk alleles:
rs2070586 A
rs2111902 G
rs3741775 C

These rs numbers also have risk alleles for reduced DAO enzyme activity, but I haven't figured out which the risk alleles are, and 23andMe may not report on them.
rs2052129
rs2268999
rs10156191
rs1049742

Besides avoiding foods that are high in histamines, you can use DAO enzyme derived from pigs as a supplement (15 minutes before meals containing histamines) to reduce your symptoms. Symptoms can vary so much, person to person, that I'll just leave you to read the article.

The sulfite information can be found at many places. What I read was "Naming the Demons" at http://www.readingtarget.com/nosulfites/naming.htm
He's a bit crazy, but in an entertaining way.

Have you checked your medications for preservatives? A friend developed significant neuropathy when he started non-refrigerated insulin with phenol and metacresol.

Best of health to you!

 

@Critterina thanks so much for your efforts to help me. I have skimmed the information and will look into it further as the histamine intolerance is sounding like it might be relevant to me.

The cause of the neuropathy remains a mystery at this stage!

Best wishes Andy

 

A new study confirming the difference in metabolism between folic acid and natural folate. They don't look at people's MTHFR status in this one.

http://ajcn.nutrition.org/content/100/2/593.full.pdf

Also, either or both folate deficiency and B12 deficiency can contribute to neuropathy

 

Special thanks to @caledonia, also @Critterina, @Sea and @taniaaust1. This is what I have learned which may explain a few things.

The theory...

MTHFR+/+, MTRR +/+ = issues with B12 depletion

I may already have been depleting B12 which may have caused neuropathy starting in right toes 2007

Metformin early 2011 = further depletes B12 and CoQ10

Statins mid and late 2011 = further depletes CoQ10

Myopathy mid and late 2011 associated with statins + ME increased severity ongoing

Neuropathy starts in right hand 2012

Of course all this time I have been checking my B12 levels via blood tests

I love PR

 

Th

 

@AndyPandy I & We love you, too, (although I can take no credit in interpreting your results )