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MicroRNA Potential Diagnostic Biomarker In NK Cells

Discussion in 'Latest ME/CFS Research' started by Never Give Up, Mar 12, 2016.

  1. Never Give Up

    Never Give Up Collecting improvements, until there's a cure.

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    http://www.ncbi.nlm.nih.gov/pubmed/26967895

    Time to study up on microRNA's.

    PLoS One. 2016 Mar 11;11(3):e0150904. doi: 10.1371/journal.pone.0150904.
    MicroRNAs hsa-miR-99b, hsa-miR-330, hsa-miR-126 and hsa-miR-30c: Potential Diagnostic Biomarkers in Natural Killer (NK) Cells of Patients with Chronic Fatigue Syndrome (CFS)/ Myalgic Encephalomyelitis (ME).
    Petty RD1,2, McCarthy NE3, Le Dieu R2, Kerr JR1,4.
    Author information
    • 1CFS Group, St George´s University of London, Cranmer Terrace, London, United Kingdom.
    • 2Centre for Haemato-Oncology, Bart's cancer institute, Queen Mary University of London, London, United Kingdom.
    • 3Centre for Immunobiology, The Blizzard institute, Queen Mary University of London, London, United Kingdom.
    • 4Grupo de Salud Publica, Escuela de Medicine y Ciencias de la Salud, Universidad del Rosario, Quinta de Mutis, Bogotá 111221, Colombia.
    Abstract
    BACKGROUND:
    Chronic Fatigue Syndrome (CFS/ME) is a complex multisystem disease of unknown aetiology which causes debilitating symptoms in up to 1% of the global population. Although a large cohort of genes have been shown to exhibit altered expression in CFS/ME patients, it is currently unknown whether microRNA (miRNA) molecules which regulate gene translation contribute to disease pathogenesis. We hypothesized that changes in microRNA expression in patient leukocytes contribute to CFS/ME pathology, and may therefore represent useful diagnostic biomarkers that can be detected in the peripheral blood of CFS/ME patients.

    METHODS:
    miRNA expression in peripheral blood mononuclear cells (PBMC) from CFS/ME patients and healthy controls was analysed using the Ambion Bioarray V1. miRNA demonstrating differential expression were validated by qRT-PCR and then replicated in fractionated blood leukocyte subsets from an independent patient cohort. The CFS/ME associated miRNA identified by these experiments were then transfected into primary NK cells and gene expression analyses conducted to identify their gene targets.

    RESULTS:
    Microarray analysis identified differential expression of 34 miRNA, all of which were up-regulated. Four of the 34 miRNA had confirmed expression changes by qRT-PCR. Fractionating PBMC samples by cell type from an independent patient cohort identified changes in miRNA expression in NK-cells, B-cells and monocytes with the most significant abnormalities occurring in NK cells. Transfecting primary NK cells with hsa-miR-99b or hsa-miR-330-3p, resulted in gene expression changes consistent with NK cell activation but diminished cytotoxicity, suggesting that defective NK cell function contributes to CFS/ME pathology.

    CONCLUSION:
    This study demonstrates altered microRNA expression in the peripheral blood mononuclear cells of CFS/ME patients, which are potential diagnostic biomarkers. The greatest degree of miRNA deregulation was identified in NK cells with targets consistent with cellular activation and altered effector function.

    PMID:

    26967895

    [PubMed - as supplied by publisher]
     
  2. alex3619

    alex3619 Senior Member

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    Logan, Queensland, Australia
    I am reading the paper as this was posted. I may have more to say later.

    One thing I find interesting is they identified some of the miRNA targets, and it looks like they found that NK cells are basically being told to hurry up and do nothing. Go, go, go, but don't do anything much.
     
    rosie26, JaimeS, mango and 8 others like this.
  3. duncan

    duncan Senior Member

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    Good to see Kerr still plugging away on our behalf.
     
  4. A.B.

    A.B. Senior Member

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  5. Janice Hargreaves

    Janice Hargreaves

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    Would it be useful to know what the eosinophils are doing miRNA wise? In my day I was taught the eosinophils released histamine. It's probably a daft question since I am hopelessly out of date....apologies if it's just too daft. Just wondering.
     
    wastwater likes this.
  6. halcyon

    halcyon Senior Member

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    Amusing that two of the other authors are from QMUL.
     
  7. Gemini

    Gemini Senior Member

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