In this sort of sequel to my previous thread, I am not currently taking Methylation related supplements, though I did experiment with B12 (methyl) and Folate (also Methyl) a few days ago, and had a few improvements in mental symptoms that have been bothering me for the past year. I believe I may be deficient in B12 which was induced by cutting Gluten out of my diet in late 2014, however I did a lot of things during that time so it's hard to tell. I stopped the supplements cold turkey, and I am going to attempt again this time with more information and cofactors. This goes out to @Freddd, I read your symptom list of PFD btw. If anyone on here is up to answering these questions, I shall lay them out. 1. Is it necessary to keep taking Methylfolate during the night to prevent Paradoxical Folate Deficiency or does it only have to be taken during the day? 2. If one is in the midst of a methyl trapping situation, would it be best to quickly increase the doses and monitor symptoms? 3. If you have a B12 or Folate deficiency, which one should be supplemented first if you are more deficient in one than the other? 4. If my symptoms stop improving or I no longer feel benefits at the dose I am at, does this mean I need to increase the dose or could it mean something else? 5. If I need to stop methylation supplements for whatever reason, do I have to go back to them at some point or will I get Paradoxical Folate Deficiency unless I keep taking them? 6. What is the best test to do for checking mineral levels within the body? I have done a hair test which shows mineral derangement (Mercury, had loads of exposures to it), so I am a little concerned about this. Also have to avoid copper since that was high on the hair test. 7. How long should I start off at a low dose of say 500mcg or 1mg (I can tolerate 1mg very well) before increasing? When I no longer feel a "high" when taking that dose? I honestly hope someone can answer some of these questions, I already asked @stridor about quite a few, so I just need to know a little bit more before I can start to plan this out again so I can do it safely with minimal risk of potentially screwing myself up.