I've tried posting previously on Dr. Amy's forum, but no one could provide any kind of insight, so I thought I might pick some brains here. There are so many inconsistencies (comparing what my values should be to what they actually are) that I'm quite confused about the whole methylation thing. I don't even react to supplements the way one would predict; I took a full month of Metanx (extremely high levels of methylfolate and P5P) before knowing anything about methylation and felt good for a month, then returned to baseline so I eventually stopped taking it. At this point, I've gone through the entire methylation cycle protocol and have experienced nothing - neither good nor bad. It's like I was taking hundreds of dollars worth of sugar pills. At this point, I'm still daily taking methylfolate and methyl B12, but I don't feel any differently, so I'm unclear if: 1) I never had a methylation problem 2) I had a screwed up methylation problem but fixed it 3) I had and still have a screwed up methylation cycle. Here is perhaps too much information: #1: I have a homozygous CBS mutation. However, Ammonia level - in BOTH blood and urine it is perfectly normal and Taurine and phenylalanine are also low: all three would be expected to be elevated in a CBS upregulation. Urine Amino Acids - ACROSS THE BOARD all are either LOW normal or just LOW, including Taurine) Sulphur - low end of normal. This correlates with my daily sulphur strips which almost always show either <200 or <400. Even the day after eating high sulphur foods (eggs) my sulphur only goes up to <800. I do get terrible headaches after having 1 glass of wine or 1 cider, which was why I initially anticipated my sulphur levels would be very high. #2: Biopterin - NORMAL Neopterine - low Neopterin/Biopterin Ratio: low I was expecting low biopterin as a result of my mutations. Supposedly, a marker for decreased BH4 is elevated phenylanine or ammonia, but both are low end of normal. So my BH4 is totally fine, which I would expect to be completely screwed up with my mutations. #3: Homocysteine level blood - very low (4 umol/l) This makes sense due to CBS, however, how can the homocysteine be pulled down the CBS drain without generating excess ammonia and sulphur? Citrulline and methionine are both low, which does indicate a CBS up regulation (even though taurine, ammonia, and phenylalanine are perfect). Thanks to anyone who throws me a bone!