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Gene expression in response to exercise in patients with chronic fatigue syndrome: a pilot study.
Andrew Keech1*, Ute Vollmer-Conna2, Benjamin K. Barry1, 4 and Andrew R. Lloyd3
1 School of Medical Sciences, University of New South Wales, Australia
2 School of Psychiatry, University of New South Wales, Australia
3 Inflammation and Infection Research Centre, University of New South Wales, Australia
4 Neuroscience Research Australia, Australia
Front. Physiol. | doi: 10.3389/fphys.2016.00421
Chronic fatigue syndrome (CFS) is a debilitating disorder of unknown pathogenesis, characterised by fatigue, which is exacerbated after minimal exercise.
We examined the effect of a single bout of aerobic exercise on leucocyte mRNA expression of genes putatively linked to exaggerated afferent signalling as an under-pinning of the fatigue state.
A carefully-characterised sample of patients with CFS (N = 10) and healthy matched control participants (N = 12) were included.
Participant ratings of fatigue and other symptoms, as well as blood samples, were obtained at baseline, and five other time-points up to 72 hours after 25 minutes of moderate-intensity cycling exercise. Leucocyte mRNA of 19 metabolite-sensing, adrenergic, immune and neurotransmission genes was examined using quantitative polymerase chain reaction.
Patients with CFS reported substantial fatigue, functional impairment and poor sleep at baseline (all p < 0.02), and exercise immediately induced worsened patients’ fatigue (effect size, ES = 1.17).
There were no significant changes in gene expression after exercise and patients did not differ from control participants at any time point.
Higher levels of expression of ficolin (FCN1) and a purinergic receptor (P2RX4) in patients with CFS were found when all time points were combined.
Patients with CFS did not show significant exercise-induced changes in leucocyte mRNA of 19 metabolite-sensing, adrenergic, immune and neurotransmission genes despite a prominent exacerbation of fatigue.
Keywords: mRNA, Pathogenesis, Myalgic encephalomyelitis, Post-exertional Malaise, Central sensitisation
Citation: Keech A, Vollmer-Conna U, Barry BK and Lloyd AR (2016). Gene expression in response to exercise in patients with chronic fatigue syndrome: a pilot study.. Front. Physiol. 7:421. doi: 10.3389/fphys.2016.00421
Received: 07 Jul 2016; Accepted: 06 Sep 2016.
Edited by:
Elisabeth Lambert, Baker IDI Heart and Diabetes Institute, Australia
Reviewed by:
Urs Nater, University of Marburg, Germany
Maureen Hanson, Cornell University, USA
http://journal.frontiersin.org/article/10.3389/fphys.2016.00421/abstract
Andrew Keech1*, Ute Vollmer-Conna2, Benjamin K. Barry1, 4 and Andrew R. Lloyd3
1 School of Medical Sciences, University of New South Wales, Australia
2 School of Psychiatry, University of New South Wales, Australia
3 Inflammation and Infection Research Centre, University of New South Wales, Australia
4 Neuroscience Research Australia, Australia
Front. Physiol. | doi: 10.3389/fphys.2016.00421
Chronic fatigue syndrome (CFS) is a debilitating disorder of unknown pathogenesis, characterised by fatigue, which is exacerbated after minimal exercise.
We examined the effect of a single bout of aerobic exercise on leucocyte mRNA expression of genes putatively linked to exaggerated afferent signalling as an under-pinning of the fatigue state.
A carefully-characterised sample of patients with CFS (N = 10) and healthy matched control participants (N = 12) were included.
Participant ratings of fatigue and other symptoms, as well as blood samples, were obtained at baseline, and five other time-points up to 72 hours after 25 minutes of moderate-intensity cycling exercise. Leucocyte mRNA of 19 metabolite-sensing, adrenergic, immune and neurotransmission genes was examined using quantitative polymerase chain reaction.
Patients with CFS reported substantial fatigue, functional impairment and poor sleep at baseline (all p < 0.02), and exercise immediately induced worsened patients’ fatigue (effect size, ES = 1.17).
There were no significant changes in gene expression after exercise and patients did not differ from control participants at any time point.
Higher levels of expression of ficolin (FCN1) and a purinergic receptor (P2RX4) in patients with CFS were found when all time points were combined.
Patients with CFS did not show significant exercise-induced changes in leucocyte mRNA of 19 metabolite-sensing, adrenergic, immune and neurotransmission genes despite a prominent exacerbation of fatigue.
Keywords: mRNA, Pathogenesis, Myalgic encephalomyelitis, Post-exertional Malaise, Central sensitisation
Citation: Keech A, Vollmer-Conna U, Barry BK and Lloyd AR (2016). Gene expression in response to exercise in patients with chronic fatigue syndrome: a pilot study.. Front. Physiol. 7:421. doi: 10.3389/fphys.2016.00421
Received: 07 Jul 2016; Accepted: 06 Sep 2016.
Edited by:
Elisabeth Lambert, Baker IDI Heart and Diabetes Institute, Australia
Reviewed by:
Urs Nater, University of Marburg, Germany
Maureen Hanson, Cornell University, USA
http://journal.frontiersin.org/article/10.3389/fphys.2016.00421/abstract