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High-Throughput Sequencing of Plasma MicroRNA in CFS/ME

Discussion in 'Latest ME/CFS Research' started by user9876, Sep 21, 2014.

  1. user9876

    user9876 Senior Member

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    A new paper from Sonya Marshall-Gradisnik at Griffith University

    Full paper is available at
    http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102783
     
    Kati, rosie26, Sasha and 4 others like this.
  2. Simon

    Simon

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    Well, sort of. Crucially, they failed to correct for multiple comparisons which is important when lots of genes are being compared:
    Had they corrected, they might have found fewer than three miRNAs different, or even none.

    Also, results like this have a habit of not replicating. Ideally, you split your sample into a 'test' set, where you look for differences, and a 'validation' set where you check the differences in the test set hold up. But there sample (20) wasn't big enough for this.

    They are also looking at miRNA in plasma. miRNA are small, regulatory RNA molecules that regulate gene expression (postranscriptionally) within the cell - quite how much clinical importance can be attached to the stuff floating free in plasma isn't clear.

    Hopefully further and bigger studies will make progress, but I'm not sure this results is terribly promising. Though I am more sceptical than most.
     
    aimossy, WillowJ, user9876 and 3 others like this.
  3. Valentijn

    Valentijn The Diabolic Logic

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    23andMe doesn't test for any SNPs on the 3 miRNA which are mentioned.
     
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  4. NK17

    NK17 Senior Member

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    This is exactly where Dr. Davis from Stanford will come to the rescue.
    He has access to well characterized biological specimens from Dr. Kogelnik's and Dr. Montoya's clinics.
    He works with Eric Delwart from UCSF as well.
    He's silently but relentlessly working to find answers, strong answers on solid data.
     
    Last edited: Sep 21, 2014
    ukxmrv, Kati, catly and 2 others like this.
  5. lnester7

    lnester7 Seven

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    USA
    Do you have his email or info to send him this link???
     
  6. Dolphin

    Dolphin Senior Member

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    lnester7 likes this.

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