Hello all, this is my first post, though I've been around for a while. I've had two Methylation Pathway Panels, 4 months apart. The second one showed good improvement just from the classic Simplified Protocol, but there is still clear methylation cycle dysfunction per Dr. Van Konynenburg's typical characterization of a Methylation block. Here is data from the first one. Everything in the second one is still out of range but slightly moved in the right direction. =============================== glutathione (oxidized) 0.56 (0.16-0.50) glutathione (reduced) 3.0 (3.8-5.5) =============================== =============================== S-adenosymethionine 216 (221-256) S-adenosylhomocysteine 55.3 (38.0-49.0) =============================== =============================== Folic Acid, active (RBC) 332 (400-1500) =============================== Mutations of note: MTHFR C677T +/- CBS C699T +/+ MTRR H595Y, K360A, A664A all +/- The strange thing is, per my blood amino acid test, I have very high Methionine. I tested at 40 micromol/L (range of 10-33). I would expect that raw material to go around the cycle to SAMe, but from my low SAMe numbers it appears as though I am throttled at conversion of Methionine to SAMe. The s-adenosylmethionine synthase enzyme (MAT) is not typically talked about in detail in terms of mutations or cofactors to this enzyme that I may need to optimize. I wonder if anyone else is in this state or has any advice.