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High levels of Nitric oxide a cloak keeping xmrv hidden

Discussion in 'XMRV Research and Replication Studies' started by Gerwyn, Feb 25, 2010.

  1. Gerwyn

    Gerwyn Guest

    Elevated NO levels are a common finding in ME/CFS sufferers.A high nitric oxide level can Keep XMRV latent or hidden and or at a very low replicative titre .This is a common effect of NO where latent viruses are concerned. I was avirtual vegetable for four years untill someone gave me rediculously high doses of IV B12 which mops up No like a sponge.I was actually markedly better within four hours.totally anecdotal but it makes me wonder if XMRV if causative does its dirty work in its latent phase
    Nitric Oxide Down-Regulates EpsteinBarr Virus Reactivation in Epithelial Cell Lines
    Purchase the full-text article



    References and further reading may be available for this article. To view references and further reading you must purchase this article.

    Xiangrong Gaoa, Masako Tajimab and Takeshi Sairenjia, 1

    a Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Nishimachi-86, Yonago, 683-8505, Japan

    b Central Clinical Laboratory, Teikyo University School of Medicine, Tokyo, 173-8606, Japan
    Received 7 December 1998;
    revised 16 February 1999;
    accepted 6 April 1999. ;
    Available online 27 March 2002.

    Abstract

    Nitric oxide (NO), a mediator of biological functions, has an antimicrobial activity against a variety of pathogens including viruses. In this study, we found that a constitutive, low level of inducible NO synthase (iNOS) mRNA was expressed in the EBV-infected gastric tissue-derived GT38 and GT39 cell lines, by analysis with the reverse transcriptionpolymerase chain reaction (RTPCR) and Southern blotting. Treatment of these cells with a specific NOS inhibitor, NG-monomethyl-Image -arginine (Image -NMMA), induced the immediate-early, EBV transactivator gene BZLF1 protein ZEBRA, suggesting a significant increase in EBV reactivation by Image -NMMA. Northern blotting demonstrated that BZLF1 and BRLF1 transcripts were also induced by 12-O-tetradecanoylphorbol-13 acetate (TPA). Meanwhile, constitutive expression of iNOS mRNA was inhibited by TPA. Image -NMMA also enhanced TPA-induced expression of the BZLF1 gene. On the other hand, a NO donor, S-nitroso-N-acetylpenicillamine (SNAP), which releases NO in an aqueous solution, inhibited the TPA-induced BZLF1 gene expression in a dose-dependent manner at both mRNA and protein levels. These results demonstrated that NO is a regulatory factor in maintaining virus latency via inhibiting EBV reactivation in the infected epithelial cells.

    Virology
    Volume 258, Issue 2, 5 June 1999, Pages 375-381
  2. markmc20001

    markmc20001 Guest

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    quitting caffine is the best thing I have ever done for my health. It took a year or more to realize the benefits though
  3. busybee

    busybee Senior Member

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    Or maybe it was the NO causing the symptoms and the NO does give us some protection??
  4. Gerwyn

    Gerwyn Guest

    removing no seems to improve symptoms whatever the connection with no seems important
  5. fairlight

    fairlight

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    Years ago a doctor in N.Y. gave me B12 and magnesium IV pushes. For two days I felt 100% normal. B12 IM shots do nothing for me. I just got a rx for nasal b12. I've since had one or two B12 IV's and it did nothing. It may have been the push and the kind of b12?
  6. natasa778

    natasa778 Senior Member

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    Gerwyn, just curious if IV b12 you had was in methylcobalamin form? is it theoretically possible that it could have acted that fast via methylation route- silencing the retroviral gene expression, as well as or in addition to mopping up NO?
    http://jvi.highwire.org/cgi/content/abstract/74/10/4679
  7. Gerwyn

    Gerwyn Guest


    Mine was the myers very much b12 and mg pushes i tink it is a combined effect
  8. natasa778

    natasa778 Senior Member

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    http://jvi.highwire.org/cgi/content/abstract/74/10/4679
  9. fairlight

    fairlight

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    I think what I got in the IV pushes was the methylcobalamin form. I've asked for it when I was in the hospital getting IVIG but they said the methylcobalamin form was only available in emergency kits for some kind of poisioning
  10. ggingues

    ggingues $10 gift code at iHerb GAS343 of $40

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    Hampton, NH
    I have recently done this, alongside with alcohol, what have you noticed? I did it so I would not lose more nutrition than necessary to help strenghthen my body after my last flare up!
  11. usedtobeperkytina

    usedtobeperkytina Senior Member

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    Since studies show our body tends to be stuck in sympathetic mode, we need to calm our CNS system down, not rev it up. This is why "uppers" are not good for us with CFS.

    Tina
  12. usedtobeperkytina

    usedtobeperkytina Senior Member

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    Clay, Alabama
    And

    And by the way, I noticed that if I have some sweet tea, our favorite beverage here in the South, before breakfast, I get the shakes.

    I like Nyquil when I have the flu. But I avoided taking any medicine during the day when I have the flu. I thought the symptoms were part of immune system getting rid of and killing the virus.

    But one day, for whatever reason, I tried Dayquil. I started having strange feelings. I called the customer service line on the bottle. I described what I was feeling. It was a disconnected but driven feeling. The bottle said adverse reactions could be sleepiness or hyperactivity.

    The lady said I definitely was having a reaction. She said that Dayquil is just Nyquil with caffeine added. Nyquil naturally has anti-flu symptoms meds that just naturally cause drowsiness. So to counter that, they pump Dayquil with caffeine. And some people can't take it.

    So when I go to doctors and the forms ask about sensitivities, I put I am sensitive to caffeine.

    Tina

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