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Help in 23andme-based methylation analysis

Discussion in 'Genetic Testing and SNPs' started by incoggg, Dec 26, 2012.

  1. incoggg

    incoggg

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  2. arx

    arx Senior Member

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  3. incoggg

    incoggg

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    Dear Arx, thank you for responding!

    Actually, this is exactly the website I used to create the table above. What I'm currently struggling with (as I'm not a biology/genetics/medicine major) is to interpret the data. I thought somebody could probably help with that.
    Thanks!

  4. adreno

    adreno 3% neanderthal

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    Tundras of Europa
    The MTRR mutation means you need methylcobalamin. The A1298C means you don't make enough BH4, so you might need that, or at least methylfolate. For BHMT you might need TMG. So you will need:

    Methylcobalamin
    Methylfolate
    B6
    B2
    TMG

    And possibly BH4. Take a look at Ben Lynch's protocol, I think it makes a lot of sense:
    http://mthfr.net/mthfr-c677t-mutation-basic-protocol/2012/02/24/

    Basically you start with low doses of methylcobalamin, maybe 1/4 of 1mg. Increase slowly to 1mg, then add methylfolate, say 200mcg. Increase that to 400mcg. If that goes well add in B6 (P5P), say 25mg. Then later B2, around 50mg, and lastly TMG, maybe 250-500mg. Good luck!
  5. arx

    arx Senior Member

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    Oh. Sorry. I don't know much about this. I hope you find people who have an idea about this. Good luck.
  6. incoggg

    incoggg

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    Thank you very much!

    I already supplement with a multivitamin that seems to have all of these. It's called Nutrient 950 by Pure Encapsulations. Here is the list of ingredients http://www.pureencapsulations.com/itemdy00.asp?t1=mvck1
    This is the best I could find.

    It has:
    riboflavin (B2) 50 mg.
    riboflavin 5’ phosphate 25 mg
    methylcobalamin (B12) 1,000 mcg.
    folate 800 mcg.
    (as Metafolin®, L-5-MTHF)
    pyridoxine HCl (B6) 25 mg.
    pyridoxal 5’ phosphate 25 mg.
    (activated B6)

    So, I seem to already get more than that. Is it too much and would it make sense to take less?

    Can you tell me what TMG and BH4 stand for? Is it Trimethylglycine?

    Thank you for the link. I took a look, but it's titled C677T.
    Doesn't it mean that it's only relevant for mutations in this particular SNP?
    I'm asking because according to 23andme I don't have mutation in this exact one.

    Thank you!

  7. arx

    arx Senior Member

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    I do have this file,though:

    A Guide to Nutrigenomic Testing by Dr. Yasko.

    Maybe it helps! I don't know the format of the table etc. but I remember reading about SNPs in the pdf file. I'll attach it.

    Attached Files:

  8. incoggg

    incoggg

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    Thank you!

    I guess it's the same to http://www.holisticheal.com/media/downloads/guide-to-nutrigenomic-testing.pdf
    ?

    I'l try to dig deeper, but I'm afraid I'm not qualified enough to figure it all out by myself...
  9. arx

    arx Senior Member

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  10. adreno

    adreno 3% neanderthal

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    Ok, that is a good multi. If you feel okay on that I would keep taking it. If you feel wired or overstimulated the dose may be too high. The methylcobalamin might not be absorbed well orally, so I would add some sublingual.

    TMG is trimethylglycine, yes. BH4 is tetrahydrobiopterin. It is made in the methylation cycle from methylfolate and cobalamin, but with a A1298C mutation that doesn't work well for you. You might try to "override" this by taking more methylfolate, if you can tolerate it. If not, you can supplement BH4 directly, but it is expensive.

    The MTHFR protocol applies to both C677T and A1298C mutations, so it should be useful for you. Luckily you don't have both of them.

    There is also a video presentation, you might want to check it out. I posted the link here:
    http://forums.phoenixrising.me/index.php?threads/mthfr-basic-protocol.20979/
  11. incoggg

    incoggg

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    Thank you very much for such a comprehensive answer!

    I'll certainly research these supplements and will watch a video.

    In a meanwhile, if you don't mind, another question.

    Why are you particularly concentrating on A1298C, is its impact more pronounced than that of other mutations?

    Should I worry about my COMT, BHMT, MTRR, and CBS mutations, are there any implications there?
    A66G is the only one that is +/+. So, is it significant?

    Thanks again!

  12. adreno

    adreno 3% neanderthal

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    All right, I'm not really an expert on these matters, and am only learning about this myself also. So, I just have some general idea of what is going on. I'll try to answer to the best of my knowledge. But really the papers written by Yasko and others will provide you with more information.

    Anything +/+ (your MTRR) is significant and must be taken seriously. COMT mutations means you are not breaking down dopamine as fast, so that might lead to overstimulation if you take too many methyl donors. You will have to go by how you feel. BHMT I don't know much about, but I understand TMG might help this pathway. CBS means your transulfation pathway is working overtime, and this means you might have problems with sulfur and ammonia.

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