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Have You Ever Had a Cholinesterase Test?

Discussion in 'Diagnostic Guidelines and Laboratory Testing' started by slayadragon, Dec 10, 2009.

  1. slayadragon

    slayadragon Senior Member

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    I'm wondering if anyone here ever has had a cholinesterase test.

    If so, what were your results?

    Did your doctor speculate on what might be responsible?

    Some information on this is below.

    Thanks much for your help!

    Lisa

    *

    Cholinesterase
    From Wikipedia, the free encyclopedia

    In biochemistry, cholinesterase is a family of enzymes that catalyze the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation.

    There are two types:

    * Acetylcholinesterase (EC 3.1.1.7) (AChE), also known as RBC cholinesterase, erythrocyte cholinesterase, or (most formally) acetylcholine acetylhydrolase, found primarily in the blood and neural synapses.

    * Pseudocholinesterase (EC 3.1.1.8) (BChE or BuChE), also known as plasma cholinesterase, butyrylcholinesterase, or (most formally) acylcholine acylhydrolase, found primarily in the liver.

    The difference between the two types of cholinesterase has to do with their respective preferences for substrates: the former hydrolyses acetylcholine more quickly; the latter hydrolyses butyrylcholine more quickly.

    In 1968, Walo Leuzinger et al. successfully purified and crystallized the enzyme from electric eels at Columbia University, NY.[1][2]

    The 3D structure of acetylcholinesterase was first determined in 1991 by Joel Sussman et al. using protein from the Pacific electric ray.[3]

    Clinically-useful quantities of butyrylcholinesterase were synthesized in 2007 by PharmAthene, through the use of genetically-modified goats.[4]

    Clinical significance

    An absence or mutation of the pseudocholinesterase enzyme leads to a medical condition known as pseudocholinesterase deficiency. This is a silent condition that manifests itself only when people that have the deficiency receive the muscle relaxants succinylcholine or mivacurium during a surgery.

    Elevation of plasma pseudocholinesterase was observed in 90.5% cases of acute myocardial infarction.[5]

    The presence of acetylcholinesterase in the amniotic fluid may be tested in early pregnancy. A sample of amniotic fluid is removed by amniocentesis, and presence of AChE can confirm several common types of birth defect, including abdominal wall defects and neural tube defects.[6]

    Butyrylcholinesterase can be used as a prophylactic agent against nerve gas and other organophosphate poisoning.[4]

    Cholinesterase inhibitors

    A cholinesterase inhibitor (or "anticholinesterase") suppresses the action of the enzyme. Because of its essential function, chemicals that interfere with the action of cholinesterase are potent neurotoxins, causing excessive salivation and eye-watering in low doses, followed by muscle spasms and ultimately death (examples are some snake venoms, and the nerve gases sarin and VX). One counteracting medication is pralidoxime.

    Among the most common acetylcholinesterase inhibitors are phosphorus-based compounds, which are designed to bind to the active site of the enzyme. The structural requirements are a phosphorus atom bearing two lipophilic groups, a leaving group (such as a halide or thiocyanate), and a terminal oxygen. The entry on Lawesson's reagent has some details on one sub-class of the phosphorus-based compounds.

    Benzodiazepines, eg temazepam have an inhibitory effect on cholinesterase.[7]

    Outside of biochemical warfare, anticholinesterases are also used for reversing medication induced paralysis during anesthesia; as well as in the treatment of myasthenia gravis, glaucoma, and Alzheimer's disease. Such compounds are used for killing insects in a range of products including sheep dip, organophosphate pesticides, and carbamate pesticides. In addition to acute poisoning as described above, a semi-acute poisoning characterized by strong mental disturbances can occur. Also, prolonged exposure can cause birth defects.
  2. wciarci

    wciarci Wenderella

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    Connecticut
    Hi Lisa,

    Just wondering about this post. Do you think there is a link with CFS? I have been tested and am positive for atypicalpseudocholinesterase. Also runs in the family, along with autoimmune issues. I was wondering if there was a link myself, if so, then how? Could it interfer with B12 or methylation? Thanks

    Wendy

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