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Griffith Uni ME/CFS Centre for NeuroImmunology & Emerging Diseases opens

Discussion in 'General ME/CFS News' started by Simon, Dec 1, 2013.

  1. alex3619

    alex3619 Senior Member

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    You can make antibodies to attach to most biological molecules. The antibodies flash when lasered, the targets they are attached to can be anything. According to Cort the modern machines can simultaneously test up to (iirc) 18 different targets simultaneously. So you can make antibodies to attach to one of the CD markers on the surface, then count how many flashes you get as the blood passes through. These lasered antibodies light up these cells like lights on a Christmas tree, which then is recorded.
     
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  2. Firestormm

    Firestormm Guest

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    So.... they are looking for evidence of infection? Current infection and/or past infection? And why this obsession with antibiotics? No worries if you don't know matey - I need to do some reading :)
     
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  3. Marco

    Marco Old blackguard

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  4. MeSci

    MeSci ME/CFS since 1995; activity level 6

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    Ah, well, @alex3619, I will just have to continue admiring you from afar! :D Even if I could manage the journey from Cornwall I might need a prolonged period of convalescence afterwards, and it sounds as though I would turn into a pool of liquid human first, making a nasty mess.

    It does all sound very promising for people living nearer or more able to travel though! :)
     
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  5. alex3619

    alex3619 Senior Member

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    You can use this to count cells like NK cells, or bright NK cells or dim NK cells. Its a diverse technology. You can count most biological molecules, though I think it works best on cells.

    This is the third (?second) time I posted this. It has been glitching, but at least it was saved.
     
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  6. Elph68

    Elph68 Senior Member

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    Hi Firestormm,

    They are saying that bacteria classed as normal flora .... are the cause of CFS, but requires further study .....

    They are asking for Aussies to go and have some blood tests for their machine .... those with CFS, and those who are normal to be used as controls.

    It is going to come out .... this is a chronic mucosal inflammation caused by in most cases, normal flora (streptococcus, enterococcus & lactobacillus) that results in a chronic auto-immune response and the subsequent complications ......

    Something like ..... Streptococcus sanguinis/parasanguinis +- enterococcus facaelis and lactobacillius vaginalis working together = chronic inflammation and CFS .... How is that for stretching the minds ....
     
  7. Firestormm

    Firestormm Guest

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  8. Elph68

    Elph68 Senior Member

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    As far as antibiotics go ..... they can be part of the cause of the problem, but are also a big part of the cure ....

    I have vancomycin + ciprofloxican + Augmentin Forte + Azithromycin for a month in order to try and run these suckers down ....
     
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  9. MeSci

    MeSci ME/CFS since 1995; activity level 6

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    Leaky gut...? :smug:
     
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  10. aimossy

    aimossy Senior Member

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    whos they @Elph68 did you have this tested through the CDD?
    I keep meaning to ask on the thread you posted because im really interested in the stuff your up to with gut etc.
    maybe I should have posted this on that thread.:)
     
  11. Simon

    Simon

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    Info on flow cytometery

    I don't think there has been any revolution in flow cytometery so I suspect that Griffith Uni simply have the most bang-up-to-date version of a technology that is constantly evolving.

    Basically it's a very powerful tool that is used primarily on whole cells to detect - and even sort - different types of immune cells based on specific surface proteins (which can include biomarkers). The flourescent antibodies Alex mentions are used to "tag" particular cell surface proteins of interest, then cells are pushed through the flow cytometry kit and each cell is then counted according to the tag on it's surface. It's used to sort out different lymphocytes eg CD4 from CD8, or Natural Killer cells (specifically looked at in the Griffith uni studies) - and even subypes of these cell groups - based on the different electrical charge on the different cells (see video below).

    Animation of flow cytometery used to sort cells


    diagram of the kit involved:
    [​IMG]
     
    Last edited: Dec 2, 2013
  12. Snow Leopard

    Snow Leopard Senior Member

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    Hey, if the CDC want to fund research at Griffith University rather than the stuff they've been doing so far...
     
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  13. aimossy

    aimossy Senior Member

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    Looks like invest in ME put money towards this tool at Griffith Uni. I saw this stated tonight on their FB page.
     
  14. Marco

    Marco Old blackguard

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    Plausible.

    Stress - leaky gut - LPS translocation - Toll like receptors - microglial activation/neuroinflammation could explain all ME/CFS symptoms.

    http://www.biologicalpsychiatryjournal.com/article/S0006-3223(12)00599-9/fulltext

    In addition, transient breakdown of the blood brain barrier or spreading neuroinflammation via the parachema could expose the central nervous system to autoimmune processes.

    Antibiotics could nip this process in the bud. The antibiotic Minocycline may have a double whammy effect on bacterial translocation and microglial activation. In theory :

    http://www.ncbi.nlm.nih.gov/pubmed/23470532

    I'd still like to see something in print though as to how this relates to ME/CFS.
     
    Last edited: Dec 2, 2013
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  15. MeSci

    MeSci ME/CFS since 1995; activity level 6

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    There are several scientific teams working in this general area (leaky gut) but not necessarily all focusing on the same culprit microorganisms:

    http://forums.phoenixrising.me/index.php?forums/the-gut-de-meirleir-maes-h2s-leaky-gut.9/
     
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  16. akrasia

    akrasia Senior Member

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    @Elph wrote:

    And this chimes nicely with Ian Lipkin's intuition that the answer to the causality of m.e. lies in the gut microbiome.

    One of the questions raised by Unger's journey is why does the CDC want to be identified with Griffith's research?

    If they are as close as you suggest associating with an impending breakthrough like this might afford some cover when the inevitable questions arise about how and why they've mangled things.
     
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  17. Elph68

    Elph68 Senior Member

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    It is a little more than just gut microbione .... it is all the mucous membranes .... Sore throat, vaginitis, prostatitis, sore eyes, nose, psoriasis, colon .... they are all attacked by these bugs giving a whole of body inflammation ....

    All the information is out there .... just nobody has linked them all together ...... YET!
     
  18. akrasia

    akrasia Senior Member

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    Thanks for the clarification.

    Marshall Gradasnik et al. received a grant from the Edward P. Evans foundation: Investigation of Immunological and Genetic Features of CFS and MDS
    Griffith University (Australia), Leads: Sonya Marshall-Gradisnik, Don Staines, Ekua Brenu

    The Edward P. Evans Foundation is supporting research that looks at the relation of m.e. to prostate cancer and MDS.
    They're worth a look.

    Does Griffith have any idea of something upstream that sets the inflammation into motion?
     
  19. alex3619

    alex3619 Senior Member

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    One of the hypotheses I have been entertaining involves translocation of LPS from the gut as the trigger that transitions from injury or infection to ME. Its not just about tolll-like receptors though. LPS triggers a wide range of things, including migration of immune cells. I have a half written blog about this that I doubt I will be finishing any time soon.
     
  20. Elph68

    Elph68 Senior Member

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    Hi Alex,

    You may like this article ....
     
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