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gene therapy and murine leukemia retrovirus

Discussion in 'XMRV Testing, Treatment and Transmission' started by Min, Nov 4, 2009.

  1. Min

    Min Senior Member

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  2. Summer

    Summer Senior Member

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    I do not know the answer to that question, but here is another article about murine leukemia retrovirus being used as a vectors. Look under "Types of viral vectors" then "Retroviruses".
  3. Min

    Min Senior Member

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    Thank you summer, I'll read it slowly to try to understand it - we could all do with degrees in molecular biology.
  4. jenbooks

    jenbooks Guest

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    Yes they've used it to cure bubble babies (SCID) and restore a working immune system both in Italy and here. New England Journal of medicine; Aiuiti in Italy and Candotti here. You need a virus as vector to bring the healthy gene into the genome. They took out some harmful parts and tested in animals for years but left in what are known as promoter sequences. Unfortunately when the virus carrying the healthy gene randomly integrated near a human oncogene in some cells it switched the oncogene on and tho the bubble babies were cured of SCID they eventually got cancer and had to be treated for that. Now they have removed those promoter sequences.

    But this is unrelated to XMRV except perhaps it also switches on oncogenes thus perhaps accounting for higher risk of cancer but I have no idea
  5. garcia

    garcia Aristocrat Extraordinaire

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    Lorenzo's Oil disease cured by retrovirus

    Someone asked about the use of retroviruses to cure disease recently. I came across this fascinating article in the Times today:

    Gene therapy could cure Lorenzos Oil brain disease


    I never gave up hope of solving the riddle of my son Lorenzos ALD
    by Augusto Odone.

    Augusto Odone is a remarkable man:
  6. jenbooks

    jenbooks Guest

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    The retrovirus did not cure the problem. Retroviruses are used as vectors to carry good genes into the stem cells and integrate into the genome there. They disable the infective parts of the virus. MLV (murine leukemia virus--similar to XMRV) has been used--but they prefer HIV for the reasons stated in the article.
  7. garcia

    garcia Aristocrat Extraordinaire

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    Ok if you want to be pedantic "cured using".
  8. jenbooks

    jenbooks Guest

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    I'm not pedantic. They have to use viruses as vectors because viruses get into the cell, and retroviruses integrate into the genome. They used adeno-associated virus--which doesn't integrate into the genome--to treat a congenital disease that causes progressive loss of vision and blindness. Because cells in the retina stay for life, they didn't need to use a retrovirus.

    The point of gene therapy is getting the correct gene into the cell. They are now working on other means than viruses but viruses will probably be the choice for many years to come.

    What is the point here? It's an entirely different field and a very wonderful one that is finally making good progress but what does it have to do with CFS/lyme/ME--unless you want to speculate on the fact that we might have single gene disorders that could have rendered us vulnerable to lyme/CFS that could be corrected by gene therapy. But we probably don't. We have a constellation of relatively common polymorphisms most likely (gene variants) that have rendered us vulnerable to certain pathogens and toxins.

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