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Gammaretroviral integration into nucleosomal target DNA in vivo (XMRV)

Discussion in 'XMRV Research and Replication Studies' started by Jemal, May 13, 2011.

  1. Jemal

    Jemal Senior Member

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    http://www.ncbi.nlm.nih.gov/pubmed/21561906

    Can someone explain this to me, what does it mean? :D
     
  2. Bob

    Bob

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    Good question! :D


    What I think I understand, so far, is that they infected human CD4(+) T lymphocytes with XMRV (or an MLV-based retroviral vector), in vivo.
    (in vivo = experimentation using a whole, living organism as opposed to a partial or dead organism, so I don't know how they went about this - they can't have infected humans with XMRV.)

    Then I think they confirm that they detected XMRV integrated into the human DNA, in a similar pattern to HIV integration. (But I'm not absolutely certain about this!)

    If they've demonstrated XMRV integration into human DNA in a living cell, then that's helpful.

    That's about all I understand!

    I don't know what the implications of their conclusion might be.
     
  3. Cort

    Cort Phoenix Rising Founder

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    Thats' what I got - they showed where in the genome XMRV integrates. Retroviruses integrate themselves into our genome and then use it to replicate. They showed where in our DNA it tends to do that....

    It shows the level of interest in XMRV (real virus) that is present, whether or not it is implicated in CFS or prostate cancer. It is a retrovirus that can infect human cells.
     
  4. Jemal

    Jemal Senior Member

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    Thanks! So does it bring anything new to the table, regaring the contamination controversy?
     
  5. Bob

    Bob

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    Another good question! :D

    That's what I was wondering...

    Is this the first piece of research that has demonstrated XMRV integration into human DNA? There was some evidence of DNA integration in relation to prostate cancer patients, but the validity of that study has now been questioned, due to questions about contamination from the cell line.

    If this is the first confirmation since then, then it does show that XMRV can fully infect humans. I think that this is helpful, and another piece of the jigsaw puzzle, but I'm not sure about how significant it is because many scientists already seem to be agreeing that human infection is possible, at least in prostrate cancer patients.

    Apart from confirming that full XMRV infection (i.e. DNA integration and therefore viral replication?) is possible in humans (quite helpful!), I don't think that it tells us anything new about the WPI research.

    What we need is a demonstration from the WPI or Alter/Lo, that they have found DNA integration in their patients.


    So, I'm not certain about the significance of this study in relation to XMRV generally.
    The evidence and knowledge does seem to be building up slowly but surely.
     
  6. August59

    August59 Daughters High School Graduation

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    I probably have this wrong, but didn't Alter/Lo find XMRV intergrated into human DNA in one end/side. At one conference Coffin was pressing Alter to find XMRV fully intergrated into human DNA as in both side/ends???
     
  7. Otis

    Otis SeƱor Mumbler

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    I'm pretty fuzzy these days but I'm pretty sure Silverman showed XMRV was integrated into DNA in one end and that Coffin's challenge to Lo/Alter was to isolate and fully sequence the PLMVs they found.
     
  8. August59

    August59 Daughters High School Graduation

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    Sounds right and makes sense to me as the fuzz has been pretty thick on me as well. Thanks
     
  9. Daffodil

    Daffodil Senior Member

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    i was told this paper would prove that XMRV is a real human pathogen that integrates into the genome and not a contaminant. i dont know if integration sites have been shown before.
     
  10. Bob

    Bob

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    That's good to hear, but that still doesn't prove that what the WPI have detected is not a contaminant.
    Unfortunately the questions of contamination in relation to the WPI will probably still remain.
    But it does add another piece to the jigsaw puzzle of knowledge about XMRV.
    I've only read the abstract though, so I don't know if there is more significant information in the full text.
     
  11. ixchelkali

    ixchelkali Senior Member

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    You know, not a day goes by without my saying that. ;)

    So now we know how and where XMRV and related gammaretroviruses integrate. We just don't know whether we should care. :D

    Seriously, it's good to see that research is proceeding. I wonder how this integration compares with that of other gammaretroviruses, like Feline Leukemia Virus?
     
  12. currer

    currer Senior Member

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    Maybe this study would help future researchers to know where to look for integration into human DNA, if they can show a preference for certain integration sites in this paper?
     
  13. Enid

    Enid Senior Member

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    It is good to see how this research is moving along now. A real mine of information on the latest here - thanks to you all.
     

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