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Functional B vitamins

Discussion in 'Detox: Methylation; B12; Glutathione; Chelation' started by Suzy, Jan 31, 2010.

  1. Suzy

    Suzy Guest

    I was wondering what is the best way to test for the functionality of B vitamins.

    Biolab in the UK will test B1,B5,B6 and biotin functionality, but is there another way.

    Is it possible by checking the level of certain metabolites ? For example, I had high hippuric and oxalic acids on my organic profile and Rich said this indicated B6 deficiency.

    Suzy
  2. Hysterical Woman

    Hysterical Woman Senior Member

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    Suzy/B tests

    Hi Suzy,

    Thanks for posting. I am just getting my feet wet with this stuff, but find it fascinating. I recently asked my doctor for the MMA urine test and serum homocysteine to get a measurement of B12. I can provide some information out of the book "Could it Be B12" concerning B12 testing. I will post this in chunks since I just typed in a complete page which I then promptly lost.:Retro mad:

    Serum Vitamin B12

    1. Cost effective when used in conjunction with the more accurate urinary MMA test. Not sufficient when used alone, because of its high degree of inaccuracy (see points 2 and 3 below).

    2. Can give false-negative results (elevated serum B12 levels in the presence of deficiency) in patients with the following conditions:

    -active liver disease
    -transcobalamin II deficiency
    -intestinal bacterial overgrowth
    -myeloproliferative disorders
    -polycythemia vera
    -chronic myelogenous leukemia
    -acute promyelocytic leukemia
    -chloral hydrate medication
    -lymphoma

    3. Can give false-positive results (low serum B12 levels in the absence of deficiency) in patients with the following conditions:

    -folate deficiency
    -pregnancy (although we question the validity of this finding, because it is possible that B12 deficiency is under-recognized in pregnancy)
    -multiple myeloma
    -excessive vitamin C intake
    -transcobalamin I deficiency

    This book, of course, is written for the general public, not for CFSers. I still think it is a good resource.

    Up next will be info regarding the Urinary MMA/Creatinine Ratio Test.

    Take care,

    HW
  3. Hysterical Woman

    Hysterical Woman Senior Member

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    More B12 tests - Urinary MMA/Creatinine Ratio Test

    Urinary MMA/Creatinine Ratio Test

    1. Elevated in vitamin B12 deficiency
    2. Elevated in some inborn errors of B12 metabolism
    3. Highly sensitive
    4. Highly specific (No known false positive or false negative results)
    5. Adjusts for poor renal function
    6. Adjusts for hypovolemia/dehydration
    7. Non-invasive
    8. No fasting required
    9. Cost Effective
  4. Hysterical Woman

    Hysterical Woman Senior Member

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    Serum MMA B12 test

    Serum MMA B 12 test

    1. Elevated in Vitamin B12 deficiency
    2. Elevated in certain inborn errors of B12 metabolsim
    3. Falsely high in renal insufficiency
    4. Falsely high in intravascular volume depletion
    5. Twelve-hour fasting required.
    6. Invasive
    7. Less sensitive and specific than urinary MMA
  5. Hysterical Woman

    Hysterical Woman Senior Member

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    Plasma Homocysteine to measure B12

    Plasma Homocysteine

    1. Elevated in vitamin B12 deficiency
    2. Elevated in folate deficiency
    3. Elevated in vitamin B6 deficiency
    4. Elevated in renal insufficiency
    5. Elevated in intravascular volume depletion

    6. Elevated in chronic diseases (hypothyroidism, system lupus erythematosus, severe psoriases, some cancers, renal failure).

    7. Elevated with the use of specific medications (Dilantin, Tegretol, nitrous oxide, methotrexate, lipid-lowering drugs [colestipol and niacin in combination with thiazide diuretics], estrogen-containing oral contraceptives).

    8. Elevated in inherited errors of methionine metabolism:
    -cystathionine B-synthase deficiency
    -methionine synthase deficiency
    -methylenetetrahydrofolate reductase deficiency

    9. Elevated in certain inborn errors of B12 metabolsim

    10. Twelve-hour fasting required
    11. Invasive
  6. Sunday

    Sunday Senior Member

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    thanks HW, this is good information, clearly put. A great resource.
  7. Hysterical Woman

    Hysterical Woman Senior Member

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    Hi Sunday,

    Yes, I think the book is a good source even tho it for "general" use and not specifically for CFS. When I told my doc I wanted an MMA test by urine he had never had one of those done and was impressed when he read the information in the book. At least he has done MMA serum test for his patients before.

    Take care,

    HW
  8. richvank

    richvank Senior Member

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    Hi, Suzy.

    The Vitamin Diagnostics lab was originally started by a guy who over several years developed methods for measuring vitamins using specific strains of bacteria that need the various vitamins. I think they still use these methods, and they offer specific tests for each of the vitamins. I don't know how it compares with what Biolab does, but it measures the bioavailable levels of the vitamins, which chemical tests do not do.

    Rich
  9. Hysterical Woman

    Hysterical Woman Senior Member

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    Hi Rich,

    Do you know if the Vitamin Diagnostics Lab is up and running in their new facility yet?

    Thanks,

    HW
  10. Suzy

    Suzy Guest

    Thanks RIch,

    I was wondering about the organic acids test. For example. hippuric acid may indicate B6 deficiency. I'm wondering if having normal levels of certain compounds would rule out deficiency. For example, would a normal hippuric acid rule out b6 deficiency.

    Or do you think doing the enzyme challenge test is better at finding deficiencies ?

    Suzy

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