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Frankenstein mice provides insights into the immunology of EBV infection

Discussion in 'Other Health News and Research' started by Ecoclimber, Sep 25, 2014.

  1. Ecoclimber

    Ecoclimber Senior Member

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    Permission to repost from Prof. G

    Charcot Project: EBV in mIce

    Frankenstein mice provides insights into the immunology of EBV infection
    . #MSBlog #MSResearch

    "This study using a tansgenic mice, or Frankenstein mice, may of interest to those of you following the evolving story of EBV in MS. EBV does not have a non-human host; it is a virus that is human specific. Therefore to create an animal model you have to make the animal humanoid.

    In this case a mouse was created in the lab to have a human-like immune system that allows it be infected with EBV. The investigators then looked at the cellular immune responses to EBV; the so called CD8+ T cell responses.

    You may recall that Professor Pender has data suggesting MSers have a deficient CD8+ cytotoxic CD8+ T cell response to EBV. In this study they show that CD8+ are responsible for controlling the EBV virus viraemia in this animal model. Whether or not this is relevant to MS is a moot point, but does provide a model system for testing vaccines. The latter is clearly what we need to do the ultimate experiment; to whether or not EBV causes MS. If vaccinating high-risk people against EBV prevents them from developing MS then we will have proved that EBV is the cause of MS.

    This is similar to HPV and cervical cancer; vaccinating girls against this virus has resulted in a fall in incidence of cervical cancer, thus proving that HPV is a cause of cervical cancer."

    "The following is a link to Professor Pender's post; it is one of the most popular all-time posts on this blog."
    Guest Post: Professor Michael Pender - Multiple Sclerosis ..., 11 Feb 2014
    Professor Michael Pender graduated from The University of Queensland in 1974 with First Class Honours in Medicine and a University Medal. Over the next six years he trained as a physician and neurologist at the Royal ...


    [​IMG]
    Wesnes et al. Adoptive Transfer of EBV Specific CD8+ T Cell Clones Can Transiently Control EBV Infection in Humanized Mice. PLoS Pathog. 2014;10(8):e1004333. doi: 10.1371/journal.ppat.1004333

    Background:
    Epstein Barr virus (EBV) infection expands CD8+ T cells specific for lytic antigens to high frequencies during symptomatic primary infection, and maintains these at significant numbers during persistence. Despite this, the protective function of these lytic EBV antigen-specific cytotoxic CD8+ T cells remains unclear.

    Methods & Results:
    Here we demonstrate that lytic EBV replication does not significantly contribute to virus-induced B cell proliferation in vitro and in vivo in a mouse model with reconstituted human immune system components (huNSG mice). However, we report a trend to reduction of EBV-induced lymphoproliferation outside of lymphoid organs upon diminished lytic replication. Moreover, we could demonstrate that CD8+ T cells against the lytic EBV antigen BMLF1 can eliminate lytically replicating EBV-transformed B cells from lymphoblastoid cell lines (LCLs) and in vivo, thereby transiently controlling high viremia after adoptive transfer into EBV infected huNSG mice.
    Conclusions:
    These findings suggest a protective function for lytic EBV antigen-specific CD8+ T cells against EBV infection and against virus-associated tumors in extra-lymphoid organs. These specificities should be explored for EBV-specific vaccine development.
     
    Last edited: Sep 25, 2014
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  2. heapsreal

    heapsreal iherb 10% discount code OPA989,

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    good find. I think the researchers have treated a small number of MS patients with good results. We just need to get them to do this to cmv and hhv6??
     
    NK17 likes this.
  3. ukxmrv

    ukxmrv Senior Member

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    Thanks for this. It's always interesting to see what Professor Pender is up to.
     
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  4. NK17

    NK17 Senior Member

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    The deadly triad: EBV - CMV - HHV6 A/B.
     
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