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Feeling Overwhelmed...Need Help Understanding Results

Discussion in 'Genetic Testing and SNPs' started by GypsyA, Aug 9, 2013.

  1. GypsyA

    GypsyA

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    USA
    Hi, I am feeling overwhelmed and need help understanding results and suggestions on how to proceed with supplements/diet for my daughter. We both have lyme and CFS, possibly other things that haven't been diagnosed. She did the 23andme test because I felt like we've tried so many things over the years and nothing has really helped her. But, even after reading through these forums and others over the past few months, I am still not understanding things enough to see what the true issues are with her genes and what steps to take with supplementations/diet. Any help/suggestions is greatly appreciated.

    =======
    I also ran her numbers through the minor allele program:

    rs28414989 at Chr1:47399879 gave 'CC' The minor allele is 'C' with a MAF = 0.00999
    rs11572233 at Chr1:60383869 gave 'AG' The minor allele is 'A' with a MAF = 0.00274.
    rs61754022 at Chr1:94528359 gave 'CT' The minor allele is 'T' with a MAF = 0.00959.
    rs63750757 at Chr2:47707876 gave 'AG' The minor allele is 'A' with a MAF = 0.00999
    rs17760364 at Chr2:189631806 gave 'AG' The minor allele is 'G' with a MAF = 0.00959
    rs6798318 at Chr3:81391273 gave 'AG' The minor allele is 'G' with a MAF = 0.00548
    rs13096071 at Chr3:100801643 gave 'CT' The minor allele is 'C' with a MAF = 0.00776
    rs3087450 at Chr3:127327309 gave 'AG' The minor allele is 'A' with a MAF = 0.00182
    rs35031397 at Chr4:6302816 gave 'CG' The minor allele is 'G' with a MAF = 0.00319
    rs34116730 at Chr5:74322982 gave 'AG' The minor allele is 'G' with a MAF = 0.00137
    rs34203073 at Chr5:76331449 gave 'AG' The minor allele is 'A' with a MAF = 0.00731
    rs10036862 at Chr5:124665264 gave 'AG' The minor allele is 'A' with a MAF = 0.00914
    rs28363488 at Chr5:138266164 gave 'AG' The minor allele is 'A' with a MAF = 0.00319
    rs13167382 at Chr5:156644542 gave 'AG' The minor allele is 'A' with a MAF = 0.00959
    rs13187882 at Chr5:156674627 gave 'AG' The minor allele is 'G' with a MAF = 0.00959
    rs1041926 at Chr6:28426296 gave 'AG' The minor allele is 'G' with a MAF = 0.00685
    rs11756938 at Chr6:29456654 gave 'CT' The minor allele is 'T' with a MAF = 0.00594
    rs34057232 at Chr6:30623069 gave 'AG' The minor allele is 'G' with a MAF = 0.00548
    rs9658135 at Chr6:35378997 gave 'AG' The minor allele is 'A' with a MAF = 0.00914
    rs9658149 at Chr6:35387538 gave 'AC' The minor allele is 'A' with a MAF = 0.00914
    rs2235076 at Chr6:102516260 gave 'AG' The minor allele is 'A' with a MAF = 0.00959
    rs2941 at Chr6:146755132 gave 'AG' The minor allele is 'A' with a MAF = 0.00594
    rs35888596 at Chr6:160543080 gave 'AG' The minor allele is 'A' with a MAF = 0.00319
    rs13232752 at Chr7:16019590 gave 'AG' The minor allele is 'A' with a MAF = 0.00639
    rs34938955 at Chr7:17338800 gave 'CT' The minor allele is 'T' with a MAF = 0.00000
    rs11772720 at Chr7:20739465 gave 'CT' The minor allele is 'C' with a MAF = 0.00731
    rs6944925 at Chr7:93262702 gave 'AC' The minor allele is 'A' with a MAF = 0.00639
    rs12705801 at Chr7:111517227 gave 'CT' The minor allele is 'C' with a MAF = 0.00091
    rs8177173 at Chr7:142561922 gave 'AG' The minor allele is 'A' with a MAF = 0.00457
    rs11781640 at Chr8:145699388 gave 'CT' The minor allele is 'T' with a MAF = 0.00137
    rs735262 at Chr9:27262761 gave 'CT' The minor allele is 'T' with a MAF = 0.00914.
    rs10971602 at Chr9:33611709 gave 'CT' The minor allele is 'T' with a MAF = 0.00822
    rs1999661 at Chr9:84603019 gave 'CT' The minor allele is 'T' with a MAF = 0.00776
    rs13286360 at Chr9:117021374 gave 'AG' The minor allele is 'A' with a MAF = 0.00822
    rs12357104 at Chr10:43587714 gave 'CT' The minor allele is 'T' with a MAF = 0.00639
    rs11191279 at Chr10:104161221 gave 'CT' The minor allele is 'T' with a MAF = 0.00319
    rs10492162 at Chr12:9664352 gave 'AG' The minor allele is 'A' with a MAF = 0.00091
    rs11564181 at Chr12:40673539 gave 'AT' The minor allele is 'A' with a MAF = 0.00914
    rs11574040 at Chr12:48276432 gave 'AG' The minor allele is 'G' with a MAF = 0.00045.
    rs17198118 at Chr12:49956941 gave 'GT' The minor allele is 'G' with a MAF = 0.00685
    rs12817914 at Chr12:78253304 gave 'CT' The minor allele is 'T' with a MAF = 0.00639
    rs534564 at Chr12:118691123 gave 'AC' The minor allele is 'A' with a MAF = 0.00776.
    rs11065126 at Chr12:120880707 gave 'AG' The minor allele is 'A' with a MAF = 0.00639
    rs2231801 at Chr14:23829490 gave 'CT' The minor allele is 'T' with a MAF = 0.00548.
    rs2582563 at Chr14:105331893 gave 'CT' The minor allele is 'T' with a MAF = 0.00999
    rs4778070 at Chr15:93568008 gave 'AG' The minor allele is 'A' with a MAF = 0.00959
    rs8176928 at Chr16:3706185 gave 'GG' The minor allele is 'G' with a MAF = 0.00457
    rs35874481 at Chr17:1537078 gave 'AG' The minor allele is 'A' with a MAF = 0.00731
    rs11571365 at Chr17:6913855 gave 'CT' The minor allele is 'T' with a MAF = 0.00914
    rs929223 at Chr17:43922897 gave 'AG' The minor allele is 'A' with a MAF = 0.00731
    rs11664907 at Chr18:61234039 gave 'AG' The minor allele is 'G' with a MAF = 0.00400
    rs2231943 at Chr19:41938222 gave 'CT' The minor allele is 'T' with a MAF = 0.00548
    rs17003493 at Chr21:15908479 gave 'AG' The minor allele is 'A' with a MAF = 0.00777
    rs8142181 at Chr22:25442817 gave 'CT' The minor allele is 'T' with a MAF = 0.00600
    rs34842534 at Chr22:29191898 gave 'AG' The minor allele is 'A' with a MAF = 0.00090
    rs11573531 at ChrX:25014016 gave 'CT' The minor allele is 'T' with a MAF = 0.00482
    rs71980227 at ChrX:43514454 gave 'DI' The minor allele is 'D' with a MAF = 0.00999
    rs4265330 at ChrX:55678960 gave 'GT' The minor allele is 'G' with a MAF = 0.00999
    rs11574627 at ChrX:70327201 gave 'CT' The minor allele is 'T' with a MAF = 0.00241.

    Attached Files:

  2. Valentijn

    Valentijn Activity Level: 3

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    Her VDR Bsm and VDR Taq aren't matching up like they should. This might indicate a significant problem, which could show up on lab tests as low calcium and/or phosphorous levels.

    If there is a problem, it can be treated with "high" doses of vitamin D3 - the ones in the neighborhood of 1000-5000 IU. Low vitamin D levels can make VDR problems even worse.
    helen1 and GypsyA like this.
  3. GypsyA

    GypsyA

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    USA
    Interesting, Valentijn!

    We have tried various supplements and protocols over the years, based on different recommendations from doctors, naturopaths, chiropractors, and our own research, etc. On a whim one day I picked up a dropper bottle of Vit D3 drops for kids, because I reasoned that we have always lived in places with little sunshine and maybe she needs more Vit D (and I figured she'd like drops better than pills). When she tried it she said she didn't like it, but over the past year she has gone back to that little bottle and said she was feeling better when she took it, but said she wasn't sure if it was from the VitD or because she was more active doing things (but, maybe she was more active/doing things because she was feeling better from the Vit D?!).

    Anyway, I will head to the store today to buy more since that bottle is empty. Do you recommend any specific brand of D3?

    Thanks, again!!!

    Love,

    Wendi
    XOXOXO
  4. Valentijn

    Valentijn Activity Level: 3

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    Nope, no specific recommendations on brand ... I don't think I've heard of major differences between any. :)
  5. GypsyA

    GypsyA

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    Great! Again, thanks so much!! I'll report back after she's been taking it for a week to let you know how she's doing with it. Did you see anything else in her results that might be helped with anything additional, or should she just stick with the VitD3 for a while on its own?
    Valentijn likes this.
  6. Valentijn

    Valentijn Activity Level: 3

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    Possibly some B12 and/or B6 would help with the MTR/MTRR and CBS. But since it's just heterozygous mutations, I doubt much is needed - maybe just what comes in a decent B complex or multi-vitamin.
    helen1 and GypsyA like this.
  7. GypsyA

    GypsyA

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    USA
    Valentijn,

    We did the 23andme testing for all three of us (me, husband, daughter--mine has to be retested they said, maybe because I'm A-neg blood type? Not sure why I have to redo the test, but they sent a new kit to me for retesting).

    My husband's test results for VDR Bsm and VDR Taq seem to be somewhat opposite of my daughters.

    Husbands: VDR Bsm TT +/+ and VDR Taq AG +/-
    Daughter's: VDR Bsm CT +/- and BDR Taq AA +/+

    Does knowing my daughter's and my husband's results allow us to extrapolate what my results will most likely be?

    I picked up the Vit D3 from the local health store just now and she took it right away. Then my husband said he wanted to take it, too. I wondered if, since his results were different from hers, if it would be beneficial/harmful/neutral for his body. Since my daughter and I are both unwell, I figured I'd take the Vit D3 along with her, to see if we both begin feeling better. What do you think about my husband and I taking the high dose Vit D3 (4,000 iu) along with her?
  8. taniaaust1

    taniaaust1 Senior Member

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    Your husband unless he is needing , shouldnt take it esp high doses of it. (the standard dose to take where I are is only 2,000 iu). Vit D3 isnt a water soluable vitamin and hence something which I assume is stored.

    Individual vitamins should never be taken without good reason (as vitamins interact with other vitamins etc etc and one can not only take much of something and cause issues, OD of vitamins can be quite toxic but one can also throw ones body out of balance).

    Most who have ME/CFS do need Vit D, often high doses.
    roxie60 likes this.
  9. roxie60

    roxie60 Senior Member

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    Your husband shuld have Vit D3,25 hydroxy test done, then treat vit D if found insufficient or defficient. Taking VitD without knowing ones status is like playing russian roullette not a good idea. B vits are soluble and if have too much will excrete them.
  10. Valentijn

    Valentijn Activity Level: 3

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    Sometimes they have trouble finding the DNA in the sample, or run into other problems with it. A- blood type shouldn't be a problem, since I'm also A- :cool:

    It's rather interesting, because even though both father and daughter have different Bsm/Taq results, both have Bsm/Taq results which don't match up with each other. People with a certain version of Bsm (homozygous major allele, heterozygous, or homozygous minor allele)almost always have the same version of Taq. So your husband and daughter not only don't each have those "matched up" results for their own Bsm vs Taq results, but they have different versions of it :p
    To some extent. We know that you have at least one Bsm C and one Taq A. Beyond that we really can't guess.
    According to the one study I saw on the subject (I've been busy and haven't looked into it more), having the Bsm vs Taq results which aren't matched up might indicate a much bigger problem than being homozygous for any particular Bsm or Taq result. Hence the D3 is probably a good idea for Mr GypsyA too.

    I've made a list of other interesting VDR variations known to have an impact of gene function at http://forums.phoenixrising.me/index.php?threads/interesting-vdr-variations.24480/ . Though that requires searching through their 23andMe genome files manually.
  11. GypsyA

    GypsyA

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    Supposedly A-negatives are one of the rarer blood types, yet I keep meeting more and more of us. In our small circle of friends, we have one A-negative for every two O-positive people (no other blood types but those two in a group of 12 individuals). I took some live blood microscopy classes, as well as bio-individuality classes, and it was interesting that a large percentage of individuals taking the classes were Rh-negative (and a large percentage of those A-). Maybe we negative-types are more inquisitive and end up bumping into each other in spaces of inquiry and learning (like this forum). :)

    That's really interesting about the results not matching up and my daughter's and husbands also having the added oddity of being opposite each other in their mismatching. Is it possible the 23andme results aren't reliable? I haven't done my retesting, but it should be interesting to see what my results are now that we see the strange the results of my husband and daughter (she's definitely biologically his child...and shows up as 50% match for his 23andme DNA results on their site...so that's not what's causing the strange mismatching).

    If you have a link handy for the study on the Bsm vs Taq mismatched study, I'd love to look over it (even though my understanding of all this, so far, is very minimal). I'd love to review it and bookmark the information to come back to after I've been able to comprehend things better.

    I'll let my husband decide if he wants to sample the D3 supplementation to see if he feels any positive effects from it, all while keeping others' advise in mind about the possibility of him overdosing. I'm thinking he'll probably want to try it out to see, since his numbers are mismatched as well as the fact that we've lived in places that have some of the lowest percentages of daily sunshine for over 20 years.

    Thanks for the VDR variations link. I don't mind searching through the 23andme results to see what I find. I'm here to learn and to help my family and myself feel as healthy as possible.

    Thanks again, Valentijn!

    XOXOXO
  12. Valentijn

    Valentijn Activity Level: 3

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    Her results are compatible with his, they're just both odd. But it's possible that the mis-matching is inherited as well.
    http://www.researchgate.net/publica..._rickets_carriers/file/79e41500e3712b4614.pdf is the study, though they're not looking directly at mismatching. The relevant table of results has been copied to The Great VDR Taq/Bsm Debate
    GypsyA likes this.

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