1. Patients launch $1.27 million crowdfunding campaign for ME/CFS gut microbiome study.
    Check out the website, Facebook and Twitter. Join in donate and spread the word!
Never Ask Us if We're Hungry -- The Answer's Always No
There are three of us here and for many years, none of us ever got hungry. When our brains would turn to mush, when our faces would go numb, and we would start the invisible vibration which is the signature dance of ME/CFS, we knew we needed to eat.
Discuss the article on the Forums.

Evolution of functional mammalian XPR1 rec. for mouse gammaretrovirus / Human XMRV

Discussion in 'XMRV Research and Replication Studies' started by August59, Sep 30, 2010.

  1. August59

    August59 Daughters High School Graduation

    Messages:
    1,480
    Likes:
    405
    Upstate SC, USA
  2. Otis

    Otis SeƱor Mumbler

    Messages:
    1,116
    Likes:
    116
    USA
    Thanks, that's interesting. Would enjoy seeing the full paper.

    J Virol. 2010 Sep 15. [Epub ahead of print]

    Evolution of functional and sequence variants of the mammalian XPR1 receptor for mouse xenotropic gammaretroviruses and the human-derived XMRV.
    Yan Y, Liu Q, Wollenberg K, Martin C, Buckler-White A, Kozak CA.

    Laboratory of Molecular Microbiology, Bioinformatics and Computational Biosciences Branch, Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.

    Abstract
    Genetic conflicts between retroviruses and their receptors result in the evolution of novel host entry restrictions and novel virus envelopes, and such variants can influence trans-species transmission. We screened rodents and other mammals for sequence variation in the Xpr1 receptor for the mouse xenotropic/polytropic (X/P-MLV) gammaretrovirus family and for susceptibility to mouse-derived X/P-MLVs and to XMRV, an X-MLV-like virus isolated from humans with prostate cancer and chronic fatigue syndrome. We identified multiple distinct susceptibility phenotypes; these include the 4 known Xpr1 variants in Mus and a novel 5th Xpr1 found in M. molossinus and M. musculus. We describe the geographic and species distribution of the Mus Xpr1 variants, but failed to find the X-MLV-restrictive laboratory mouse allele in any wild mouse. We used mutagenesis and phylogenetic analysis to evaluate the functional contributions made by constrained, variable, and deleted residues. Rodent Xpr1 is under positive selection indicating a history of host-pathogen conflicts; several codons under selection have known roles in virus entry. All non-Mus mammals are susceptible to mouse X-MLVs, but some restrict other members of the X/P-MLV family and the resistance of hamster and gerbil cells to XMRV indicates that XMRV has unique receptor requirements. We show that the hypervariable fourth extracellular XPR1 loop (ECL4) contains 3 evolutionarily constrained residues that do not contribute to receptor function, we identify 2 novel residues important for virus entry (I579, T583), and we describe a unique pattern of ECL4 variation in the 3 virus-restrictive Xpr1 variants found in MLV-infected house mice; these mice carry different deletions in ECL4 suggesting either that these sites or loop size affects receptor function.

    PMID: 20844050 [PubMed - as supplied by publisher]

See more popular forum discussions.

Share This Page