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Electron microscopic immunocytological profiles in chronic fatigue syndrome

Discussion in 'XMRV Research and Replication Studies' started by Snow Leopard, Oct 2, 2010.

  1. Snow Leopard

    Snow Leopard Senior Member

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    Electron microscopic immunocytological profiles in chronic fatigue syndrome
    Holmes et al. 1997.

    It has been suggested that this paper may have shown MLV rather than the speculated Lentivirus, which was suggested based on the reverse-transcriptase activity.

    Abstract

    Structures consistent in size, shape and character with various stages of a Lentivirus replicative cycle were observed by electron microscopy in 12-day peripheral-blood lymphocyte cultures from 10 of 17 Chronic Fatigue Syndrome patients and not in controls. Attempts to identify a lymphoid phenotype containing these structures by immunogold labelling failed and the results of reverse-transcriptase assay of culture supernatants were equivocal. The study was blind and case-controlled, patients being paired with age, sex and ethnically matched healthy volunteers. Prescreening of subjects included the common metabolic and immunological disorders, functional conditions and a virus-screen against hepatitis B and C, Epstein-Barr Virus, Cytomegalovirus and Human Immunodeficiency Virus.
     
  2. ukxmrv

    ukxmrv Senior Member

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    Andrew, I checked with Dr Holmes and he is still saying the particles he saw appeared to be Viisna virus and not MLV.

    The poor man has had to retire with very poor health.

    Mindy K reported that a colleague of Dr Holme's still has some samples so I hope that can be looked at agaon.
     
  3. Snow Leopard

    Snow Leopard Senior Member

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    Have a look at the article in the Library. The actual identity of the virus is not completely clear.
     

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