When the 'Holiday Season' Is No Holiday at all for Those With ME/CFS
Is December getting to you? Jody Smith shares some thoughts on some of the struggles that all too often attend this time of year ...
Discuss the article on the Forums.

Effect of n-3 polyunsaturated fatty acids on methylation enzymes

Discussion in 'Detox: Methylation; B12; Glutathione; Chelation' started by nanonug, Apr 15, 2012.

  1. nanonug

    nanonug Senior Member

    Virginia, USA
    This might be of use for those doing methylation protocols. What strikes me is that omega-3 fatty acids end up decreasing homocysteine levels by essentially up-regulating a portion of the transsulfuration pathway and down-regulating the methylation pathway. I am not sure that this is an altogether good thing.

    "Effect of n-3 polyunsaturated fatty acid on gene expression of the critical enzymes involved in homocysteine metabolism"


    Background: Previous studies showed that plasma n-3 polyunsaturated fatty acid (PUFA) was negatively associated with plasma homocysteine (Hcy).

    Objective: We investigated the regulatory effect of n-3 PUFA on mRNA expression of the critical genes encoding the enzymes involved in Hcy metabolism.

    Methods: HepG2 cells were treated with docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), alpha-linolenic acid (ALA) respectively for 48 h. The cells were collected and total RNA was isolated. The mRNA expression levels of the genes were determined by using Real Time-PCR.

    Results: Compared with controls, the mRNA expression levels of 5-methyltetrahydrofolate reductase (MTHFR) were significantly increased in the DHA group (p < 0.05) and ALA group (p < 0.05); Significantly down-regulated mRNA expression of methionine adenosyltransferase (MAT) was observed with the treatments compared with the controls; the level of MAT expression was significant lower in the DHA group than the ALA group (p < 0.05); Cystathionine-g-lyase (CSE) expression was significantly increased in the DHA (p < 0.05) and EPA groups (p < 0.05) compared with control. No significant changes were shown in mRNA expression levels of S-adenosylhomocysteine hydrolases (SAHH), cystathionine b-synthase (CBS), and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR).

    Conclusions: Our results suggest that DHA up-regulates CSE and MTHFR mRNA expression and down-regulates MAT mRNA expression involved in Hcy metabolism.
    ppodhajski likes this.
  2. bond


    @nanonug - I'm not sure if your conclusion is correct - they say upregulates MTHFR which is the methylation pathway and CSE but not CBS, the latter standing above CSE in the transsulfuration pathway.

    I guess Ben Lynch is relying on that study for his StrateGene reports.

    Personally I've definetely felt the upregulating effects of DHA.

    From the video above another interesting is cocoa :cautious:
  3. bond


    Wonder if the culpit is the DHA(or ALA as Ben Lynch suggests) or the Omega-3 to omega-6 ratio :confused:

See more popular forum discussions.

Share This Page