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Dr O'Keefe - Paper accepted for publication

Discussion in 'XMRV Research and Replication Studies' started by VillageLife, Aug 15, 2012.

  1. VillageLife

    VillageLife Senior Member

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    RustyJ and Bob like this.
  2. FancyMyBlood

    FancyMyBlood Senior Member

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    I don't think this paper has anything to do with MLVs honestly. It's quite common that labs are involved in several different research projects. If it was really about MLVs I'd quess such a signifcant finding would be mentioned in the title.
  3. currer

    currer Senior Member

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    This is unlikely to be about a virus other than MLVs given what we know from Dr O'Keefe's blog.
  4. RustyJ

    RustyJ Contaminated Cell Line 'RustyJ'

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    Just like the Italian paper?

    On the face of it. MRVs might not be mentioned in the paper, but there is little doubt that is what they are finding, as O'Keefe has mentioned previously in relation to this work. What did she say: not XMRV, but related? Sounds awfully like an MRV to me.
  5. Firestormm

    Firestormm Senior Member

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    Cornwall England
    Activation of innate anti-viral immune response genes in symptomatic benign prostatic hyperplasia

    'A big CONGRATS to Allison Atwood-Madigan in the lab, the above paper has just been accepted for publication in Genes & Immunity (a Nature journal)! Bring on the celebratory laser-tag'

    Has to be worth celebrating wherever one gets a paper published I would imagine, or whatever it's content.
  6. FancyMyBlood

    FancyMyBlood Senior Member

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    It might be something like the Rnase L findings earlier implicated in prostate cancer. Yes, O'Keefe may believe these finding can be explained by MLVs, but until she publishes a paper where MLVs are actually found in PC, her findings are replicated and she's able to demonstrate cause-and-effect I'll just take the paper as it is: activation of innate anti-viral immune response genes in PC.
  7. RustyJ

    RustyJ Contaminated Cell Line 'RustyJ'

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    As far as I know, MRVs have already been found in PC, so if O'Keefe's lab was to publish a paper on the discovery of MRVs, it would in fact be a replication of the earlier studies. Besides, it's not PC in this case.but benign prostatic hyperplasia.

    BTW I didn't dispute your first comment about whether MRVs were in the study, rather your comment about the study title not referring to MRVs. I was making the observation that previous studies have found MRVs, but gone to some some length to say the opposite in the title, and the abstract of the study.

    I might also mention that O'Keefe has stated that papers finding MRVs are having trouble getting published.
  8. In Vitro Infidelium

    In Vitro Infidelium Guest

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    In science, replication requires that the reproducibility of results is demonstrated via research that is independent of earlier work - a different study by the same researcher doesn't meet the 'independent' qualification. For useful points on replication and reproducibility see: http://www.sciencemag.org/content/334/6060/1225.full (multiple articles in right hand menu (may require login) and http://sciencecareers.sciencemag.or...s_issues/articles/2011_12_02/caredit.a1100133

    For the cart and horse problem in cancers see: ftp://130.94.180.226/pub/pca/inflammation/palapattu_etal_carcinogenesis_2004.pdf

    IVI
    Firestormm and barbc56 like this.
  9. Mula

    Mula Senior Member

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    Reproducibility is a test of precision when run within another setting.
  10. Daffodil

    Daffodil Senior Member

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  11. Mula

    Mula Senior Member

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    Comparatively few sequences have been completely cloned, with most being similar to the reference sequence of xmrv. When pol and env are not detectable using primers targeting these sequences, then the sequence of the primers is not the type which is present.
  12. VillageLife

    VillageLife Senior Member

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  13. Mula

    Mula Senior Member

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    Dr Lipkin is not using next generation sequencing (NGS) to look for retroviruses in MECFS patients. He is to use it to look for EBV, HHV-6, about thirty in total, for the CFI project.
  14. Daffodil

    Daffodil Senior Member

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    Thanks, Mula. So, if finding a new pathogen depends on the sequences we already know of, how can we find new ones? What if its a new pathogen with completely different sequences?

    sorry perhaps this is a dumb question
  15. Mula

    Mula Senior Member

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    It is typical to pull out one sequence with a particular test and differing versions with others. As is found with the retroviruses no two sequences are produced from a single test. To then find unknown sequences that infect below the ability of PCR next generation sequencing is the preferred choice. When a sequence is identified this can be searched for using primers that match, and if they are unknown then SISPA primers are used. Ideally both are required in this scenario.

    The same pathogen can have different sequences both identified and unknown. Classification of sequences however could then be many years in discovery.

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