Discussion in 'Latest ME/CFS Research' started by carolinetanderson, Mar 22, 2011.
Can you paste it here please? My office firewall is blocking that site.
Current problems at that link Funk.
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Disease Associations of XMRV and MLV-Related Viruses
Judy A. Mikovits, PhD, Whittemore Peterson Institute for Neuro-Immune Disease
A new human retrovirus first associated with QQ variant RNaseL a gene in the hereditary prostate cancer gene locus, in prostate cancer in 2006. Detection of integration sites in unmanipulated prostate tissue; demonstration of neutralizing antibody and in situ hybridization for XMRV, supports XMRV as a human retroviral infection associated with prostate cancer.
Although in the absence of direct sequencing it cannot be rule out the reactivity can be to related MLV viruses.
In 2009 using a classical virology approach of viral isolation and transmission, electron micropscopy, serology and PCR, Lombardi et al demonstrated the first isolation of XMRV from blood from patients with chronic fatigue syndrome (CFS) predominately from the west coast of the United States.
In 2010, Lo et al. extended these studies by detecting nucleic acids of MLV-related variants in the peripheral blood mononuclear cells of CFS from the northeastern United States suggesting additional strains capable of infecting humans exist.
In a study of 300 CFS patients, 13 developed lymphoproliferative disorders. Of those tested, 11/11 were positive for XMRV and 9/9 positive for clonalTCR gamma rearrangements. Spontaneous development of four immortalized B cells lines occurred during culture of cells from CFS patients. Three developed from B cells isolated from the peripheral blood (two of whom had B cell lymphoma) and one from a bone marrow biopsy. The B cell lines have a mature CD20+, CD23+ phenotype and produce infectious XMRV.
Virus production occurred despite extensive hypermutation of the proviruses in these cells by APOBEC3G. Therefore, XMRV infection may accelerate the development of B cell malignancies by either indirect chronic stimulation of the immune system and/or by direct infection of the B-cell lineage.
Since viral load in peripheral blood is low, these data suggest that B cells in tissues such as spleen and lymph nodes could be an in vivo reservoir for XMRV.
We have also identified an inflammatory cytokine and chemokine signature that distinguishes XMRV infected CFS patients from healthy controls with 94% sensitivity and specificity Monitoring immune dysfunction affords the opportunity to begin to understand the pathogenesis of XMRVs.
In addition to these data, recent advances in developing tests for detection and characterization of XMRVvariants will be also be discussed
Can't stop this train...
It's amazing that she can't get her studies published. Does anyone know how many she's sitting on waiting to come out?
Thanks to you all for this info and "good" reading - we're well on the way now - thanks Dr M.
Thank you Ocean Blue and carolinetanderson.
Agree FunkOdessey......maybe this indicates that it wont be stoppin at all stations in the pooring rain anymore either.
I wonder why they have not determined yet wheter those are reservoirs or not. Is that so difficult and time consuming to do? Or maybe they don't want to share this information yet?
Don't think it's hard to do, just more invasive.....would probably involve fine needle aspiration and or tissue biopsy. The later would involve a minor operation, I would think. But they do mention that two ppl had a B cell lymphoma, so one would think they could easily access those patients biopsied tissues (if they had tissue biopsied for cancer diagnosis and treatment) to look for XMRV. Perhaps they did so?
I hope so...
Can anyone explain this bit in lay language? It would be much appreciated.
TCR = T-Cell receptor. Clonal selection is an active immunological selection proces and is explained here: http://en.wikipedia.org/wiki/Clonal_selection
But this process can go wrong, resulting in a lymphoma or T-Cell leukemia.
The Gamma region is discussed here: http://en.wikipedia.org/wiki/TRG@
The immortalised cell lines are what is used in Cell culture. http://en.wikipedia.org/wiki/Cell_culture
If you take a series of human cells, place them in a test tube and give them essential nutrients, they will merely die. Cancer cells are unique because they will keep replicating, but that is not sufficient for survival as a cell line in a test tube. As far as I know, most immortal cell lines have evidence of viral involvement, often retroviral involvement.
Thanku for all the info and the links Snow Leopard...
(After I've read all of those Wiki pages, will I be eligible for a degree in cellular biology?!?!)
Dr Mikovits uses the LN Cap cell line.
Can anyone provide proof that this shows evidence of retroviral involvement particularly MLV's or XMRV as this is what is being claimed of other not used by the WPI cell lines?
You can also try a Google Site Search
Separate names with a comma.