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Detection of Urine Metabolites in a Rat Model of Chronic Fatigue Syndrome before and after Exercise

Discussion in 'Latest ME/CFS Research' started by Kati, Apr 20, 2017.

  1. Kati

    Kati Patient in training

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    Detection of Urine Metabolites in a Rat Model of Chronic Fatigue Syndrome before and after Exercise

    Shao C1, Ren Y2, Wang Z2, Kang C3, Jiang H1, Chi A2.
    Author information
    1.College of Arts and Sciences, Shanghai Maritime University, Shanghai 201306, China.
    2.Laboratory of Nutrition and Hygiene, Shaanxi Normal University, Xi'an 710119, China.
    3. School of Sports, Hebei Normal University, Shijiazhuang 050090, China.



    Abstract
    Purpose. The aim of the present study was to elucidate the metabolic mechanisms associated with chronic fatigue syndrome (CFS) via an analysis of urine metabolites prior to and following exercise in a rat model.

    Methods.
    A rat model of CFS was established using restraint-stress, forced exercise, and crowded and noisy environments over a period of 4 weeks.

    Behavioral experiments were conducted in order to evaluate the model.

    Urine metabolites were analyzed via gas chromatography-mass spectrometry (GC-MS) in combination with multivariate statistical analysis before and after exercise.

    Results.
    A total of 20 metabolites were detected in CFS rats before and after exercise.

    Three metabolic pathways (TCA cycle; alanine, aspartate, and glutamate metabolism; steroid hormone biosynthesis) were significantly impacted before and after exercise, while sphingolipid metabolism alone exhibited significant alterations after exercise only.

    Conclusion.
    In addition to metabolic disturbances involving some energy substances, alterations in steroid hormone biosynthesis and sphingolipid metabolism were detected in CFS rats.

    Sphingosine and 21-hydroxypregnenolone may be key biomarkers of CFS, potentially offering evidence in support of immune dysfunction and hypothalamic-pituitary-adrenal (HPA) axis hypoactivity in patients with CFS.

    Note: Bolding mine. Link to article: click title.
     
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  2. A.B.

    A.B. Senior Member

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    Poor rats were tortured and this was labelled "CFS model".

    I find it difficult to believe they could find the right abnormalities with a nonsensical CFS model.

    I'm guessing that the researchers knew what they were supposed to find and got there somehow.
     
    Last edited: Apr 20, 2017
    TiredSam, merylg, Izola and 19 others like this.
  3. BurnA

    BurnA Senior Member

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    Yes, I would be curious about that model alright. So they stressed the rats by the sounds of it.
     
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  4. Marky90

    Marky90 Science breeds knowledge, opinion breeds ignorance

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    Sorry but, what the fck? They get paid for this nonsense?

    You can`t impose our disease process on others by torturing them,
    this doesnt even make any sense. Poor rats
     
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  5. Effi

    Effi Senior Member

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    They created a mouse model of overtraining syndrome, not CFS (and not even chronic fatigue).
     
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  6. A.B.

    A.B. Senior Member

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    For restraint-stress, rats were fixed individually in a polyvinyl chloride tube (20.0 cm in length, 5.0 cm in diameter) for 4 h.

    For forced exercise, the rats were forced to run on a treadmill (20 m/min) for 1 h.

    The 10 rats in the CFS group were housed together in a standard rearing cage (crowded environment), whereas those in the control group were housed in individual cages.

    Rats of the CFS group were also exposed to rock music for 12 h each day (noisy environment).
     
  7. Marky90

    Marky90 Science breeds knowledge, opinion breeds ignorance

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    Ugh. Sickening.. Without any good reason for that type of research, it shouldnt be allowed.
     
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  8. Jonathan Edwards

    Jonathan Edwards Senior Member

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    I am not sure that this is convincing if they did not give the rats questionnaires to see if they were tired all the time and found the music made them even more tired. The rats I know rather like living in PVC tubes (better than sewers) and love running about.

    Do you think there is a computer programme that checks what US scientists have found and invents results the next day in China for submission to a journal of choice by lunchtime?
     
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  9. A.B.

    A.B. Senior Member

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    I hope not. The engineers sufficiently skilled for writing such a program have better things to do. ;)
     
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  10. ash0787

    ash0787 Senior Member

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    Was going to say, since when was there examples of animals with CFS, I thought perhaps it was a computational model ... I guess the chinese really will copy anything
     
  11. HowToEscape?

    HowToEscape? Senior Member

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    Humans endure far, far harsher conditions. Have you ever been in in New York City subway car during rush hours? Now think of one without air conditioning. Navy SEALs are required to go five days without sleep during training.
    Running and confined spaces are pretty normal things for rats, in any case.

    This was a completely useless study, but let's not throw a hissy fit about research in general.
     
    Last edited: Apr 20, 2017
    RogerBlack likes this.
  12. A.B.

    A.B. Senior Member

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    They could have tested patients instead of rats and generated useful data. The value of this study seems to be below zero. It's just going to confuse and distract people while being a waste of resources.
     
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  13. Sidereal

    Sidereal Senior Member

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    What the hell is this BS.
     
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  14. Marky90

    Marky90 Science breeds knowledge, opinion breeds ignorance

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    10 hours of rock music is not a normal environment, neither is the crowded cages. Your analogies does not fit very nicely. I would very much prefer being in a traffic jam (which you have other places than NYC fyi)
     
  15. Cheesus

    Cheesus Senior Member

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    I agree. The rats were essentially subjected to torture.
     
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  16. CFS_for_19_years

    CFS_for_19_years คภภเє ɠรค๓թєl

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    Oh......so this is why I came down with ME/CFS. Standard college dormitory effect combined with running to classes on a large campus and daily competitive swimming practice, circa 1972-74 (came down with ME/CFS 1990). (No restraint stress involved.)
     
  17. lnester7

    lnester7 Seven

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    Can you transfuse blood to a rat from a person? That might work.
     
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  18. HowToEscape?

    HowToEscape? Senior Member

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    There's just a tad of difference between being packed into a subway car versus being in a traffic jam. You can't replicate the conditions of the subway car in a passenger car; to begin with it's illegal to put that density of people into an automobile, and you can't stand up while in one, as that's also illegal. Except perhaps in Texas.

    In years past (USA) and now (numerous countries) they exist work environments with noise exposure greater than rock music.
     
    Last edited: Apr 20, 2017
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  19. Marky90

    Marky90 Science breeds knowledge, opinion breeds ignorance

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    Are u seriously gonna claim that a voluntary intermittent ride in a subway car equals being put in a crowded 20 cm cage against your "will", whilst being subjected to extremely loud continuous alien noise (from their point of view)? You dont spend 4 hours in a subway car, and rats cant use headphones.

    During my time in the army i had to torture people under elite training, and I can tell you they would have it a 100 times better in the scenario ur describing.
     
  20. Basilico

    Basilico Florida

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    Methods. A rat model of CFS was established using restraint-stress, forced exercise, and crowded and noisy environments over a period of 4 weeks.



    If scientists still don't understand what CFS/ME actually is, or even have a test that can identify it, how could they possibly expect to "recreate" it?

    How could they assume that stressing rats out for a month is the equivalent to having CFS/ME? This sounds like a complete waste of money and time. Any results they find will be useless because they are not working with a CFS/ME model.
     
    Cohen2, merylg, slysaint and 7 others like this.

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