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Death following HPV vaccine - persistent viral DNA found postmortem

natasa778

Senior Member
Messages
1,774
Interesting finding, raises possibility of at least one possible mechanism behind adverse vaccine reactions ...

Detection of human papillomavirus L1 gene DNA fragments in postmortem blood and spleen after Gardasil® vaccination—A case report

http://www.scirp.org/journal/PaperInformation.aspx?paperID=25840


… According to the documents presented at the inquest, the patient experienced temperament changes shortly after the first dose of Gardasil® injection, started to have dizziness spells, pins-and-needles feelings in her hands, memory lapses and abdominal pains after the second injection, and developed intermittent weak arm, frequent tiredness requiring daytime naps, increased pins-and-nee- dles feelings in hands causing things to drop from hands, appetite increase with no weight gain, night sweats, loss of ability to use common objects, intermittent chest pain and sudden unexpected “racing heart”.


The current study shows that only HPV-16 L1 gene DNA was detected 6 months after last vaccination, further suggesting that the non-B-conformation has protected the HPV-16 L1 gene DNA fragments from being degraded by various nucleases in the human body. Unprotected foreign DNA fragments in B conformation introduced into peripheral blood of a mammalian host are known to be degraded and eliminated within 48 hours [28].

HPV-16 is a virus which only infects human mucosal epithelial cells. HPV-16 DNA may be detected in the plasma of patients with invasive squamous cervical can- cer harboring the same genotype of virus, but not in the control subjects without cervical cancer [29]. HPV-16 DNA has been reported to be present in peripheral blood mononuclear cells from human immunodeficiency virus (HIV)-infected pediatric patients and even from healthy blood donors [30]. However, unlike the HPV-16 L1 gene DNA fragments found in the Gardasil® vaccine and in the postmortem materials in this autopsy case, the HPV- 16 L1 gene DNA in those reported clinical samples is always in B conformation which is readily amplified by a pair of degenerate or consensus PCR primers from both ends defined by the MY09 and MY11 binding sites [30].

The HPV-16 L1 gene DNA fragments detected in the postmortem blood and splenic tissue in this case are presumably in minute quantities and in the nucleated cells, probably macrophages. Naked viral and bacterial DNA fragments firmly bound to insoluble aluminum salts can be carried into tissue macrophages through phagocytosis to initiate a series of DNA-related immune reactions [31-34]. Intramuscular injection of free HPV-16 L1 plasmid DNA in BALB/C mice without adjuvant has been known to induce a strong CD8 T cell response [35], indicating that under certain conditions non-replicating HPV L1 gene DNA can activate the immune system. However, to be detectable 6 months after intramuscular injection, the naked foreign DNA in the host must be in a stabilized physical condition, either by remaining bound to the AAHS nanoparticles or by integration into the human genome through hitherto poorly understood mechanisms [36-40].

The presence of HPV-16 L1 gene DNA fragments of a vaccine origin indicates possible co-existence of other companion microbial DNA, such as DNA fragments of the plasmid pGAL110 and yeast cells which are used in the vaccine production by the manufacturer [2]. A potential consequence of these viral and microbial DNA fragments with their unmethylated CpG motifs in macrophages [41-46] is to cause release of various cytokines, including tumor necrosis factor (TNF), a recognized myocardial depressant [47-51]. TNF-induced hypotensive shock is a documented observation among animals [52,53] and humans [54,55]. To answer the question whether the quantity of these persistent viral or microbial DNA fragments can stimulate the macrophages to release enough TNF to generate a significant pathophysiological impact following Gardasil® vaccination needs expanded research.
 

Snow Leopard

Hibernating
Messages
5,902
Location
South Australia
This mechanism seems to be specific to this vaccine, but it is worrying for all of those people receiving it!

Something to do with the delivery method meant that the vaccine DNA had remained in tact over 6 months down the track. (unexpected and worrying!)

I actually feel this could lead to long term consequences (including autoimmunity, death) of this vaccine that might not be applicable to traditional vaccines.

The question is, was this a unique finding in this patient or is it common>
 

currer

Senior Member
Messages
1,409
Thanks for posting this Nastasa - it is really worrying.

I feel so sorry for the poor girl in this case and for her family.

We need further studies like this one looking for the traces of vaccine DNA and the other elements that make up the vacine - in this case yeast cells- whenever an adverse reaction to any vaccine is suspected - not only fatalities.
I doubt many studies are ever done.

DRUG CLASS AND MECHANISM: Gardasil is a human papillomavirus (HPV) vaccine. Gardasil is a sterile preparation for intramuscular injection and contains purified inactive proteins from HPV types 6, 11, 16, and 18. The proteins in Gardasil are structural, virus-like proteins (VLP) that resemble the HPV virus. The proteins can activate the immune system but cannot give rise to replicating virus. Viral proteins used in Gardasil are manufactured in yeast cells (S. cerevisiae) using recombinant technology. Once released from yeast cells, the VLPs are purified, combined with a catalyst (amorphous aluminum hydroxyphosphate sulfate) and a purification buffer.
 

August59

Daughters High School Graduation
Messages
1,617
Location
Upstate SC, USA
There is a lot that they do not even know about this virus yet. It is not looked upon as being a purely sexually transmited disease. It has been known to be completely put into dormancy in healthy people within 2 years of catching it. On the other hand it has be known to be the cause of various head and neck cancers 20 years after considered to be in dormancy. A big question is mutancy as there are already 10 - 15 strains of this virus, but 16 and 18 are the only ones to show up in tumors. It may turnout to be that the vaccine is useless at this point. The odd thing is that the tumors that show to have been formed by HPV 16 or 18 respond very well to radiation treatment and are up to about a 95% cure rate with no recurrencies for at least 5 years.

If it infects one side of the throat /tonsils the opposite side is never infected, which I thought was really weird. This again goes to show that this virus was not well researched or is mutating at significant rate. It's definitely transferred from mother to child. It is mostly considered to be transmitted other than sexually, out of all these buts comes all the different strains.