Interesting finding, raises possibility of at least one possible mechanism behind adverse vaccine reactions ...
Detection of human papillomavirus L1 gene DNA fragments in postmortem blood and spleen after Gardasil® vaccination—A case report
http://www.scirp.org/journal/PaperInformation.aspx?paperID=25840
Detection of human papillomavirus L1 gene DNA fragments in postmortem blood and spleen after Gardasil® vaccination—A case report
http://www.scirp.org/journal/PaperInformation.aspx?paperID=25840
… According to the documents presented at the inquest, the patient experienced temperament changes shortly after the first dose of Gardasil® injection, started to have dizziness spells, pins-and-needles feelings in her hands, memory lapses and abdominal pains after the second injection, and developed intermittent weak arm, frequent tiredness requiring daytime naps, increased pins-and-nee- dles feelings in hands causing things to drop from hands, appetite increase with no weight gain, night sweats, loss of ability to use common objects, intermittent chest pain and sudden unexpected “racing heart”.
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The current study shows that only HPV-16 L1 gene DNA was detected 6 months after last vaccination, further suggesting that the non-B-conformation has protected the HPV-16 L1 gene DNA fragments from being degraded by various nucleases in the human body. Unprotected foreign DNA fragments in B conformation introduced into peripheral blood of a mammalian host are known to be degraded and eliminated within 48 hours [28].
HPV-16 is a virus which only infects human mucosal epithelial cells. HPV-16 DNA may be detected in the plasma of patients with invasive squamous cervical can- cer harboring the same genotype of virus, but not in the control subjects without cervical cancer [29]. HPV-16 DNA has been reported to be present in peripheral blood mononuclear cells from human immunodeficiency virus (HIV)-infected pediatric patients and even from healthy blood donors [30]. However, unlike the HPV-16 L1 gene DNA fragments found in the Gardasil® vaccine and in the postmortem materials in this autopsy case, the HPV- 16 L1 gene DNA in those reported clinical samples is always in B conformation which is readily amplified by a pair of degenerate or consensus PCR primers from both ends defined by the MY09 and MY11 binding sites [30].
The HPV-16 L1 gene DNA fragments detected in the postmortem blood and splenic tissue in this case are presumably in minute quantities and in the nucleated cells, probably macrophages. Naked viral and bacterial DNA fragments firmly bound to insoluble aluminum salts can be carried into tissue macrophages through phagocytosis to initiate a series of DNA-related immune reactions [31-34]. Intramuscular injection of free HPV-16 L1 plasmid DNA in BALB/C mice without adjuvant has been known to induce a strong CD8 T cell response [35], indicating that under certain conditions non-replicating HPV L1 gene DNA can activate the immune system. However, to be detectable 6 months after intramuscular injection, the naked foreign DNA in the host must be in a stabilized physical condition, either by remaining bound to the AAHS nanoparticles or by integration into the human genome through hitherto poorly understood mechanisms [36-40].
The presence of HPV-16 L1 gene DNA fragments of a vaccine origin indicates possible co-existence of other companion microbial DNA, such as DNA fragments of the plasmid pGAL110 and yeast cells which are used in the vaccine production by the manufacturer [2]. A potential consequence of these viral and microbial DNA fragments with their unmethylated CpG motifs in macrophages [41-46] is to cause release of various cytokines, including tumor necrosis factor (TNF), a recognized myocardial depressant [47-51]. TNF-induced hypotensive shock is a documented observation among animals [52,53] and humans [54,55]. To answer the question whether the quantity of these persistent viral or microbial DNA fragments can stimulate the macrophages to release enough TNF to generate a significant pathophysiological impact following Gardasil® vaccination needs expanded research.