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Cryptovirus found in 96% of ME/CFS patients (old research news from 2006)

Discussion in 'Latest ME/CFS Research' started by Bob, Aug 14, 2010.

  1. Bob

    Bob

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    I've just come across this info on the National CFIDS Foundation website, from 2006, about the discovery of an RNA virus, 'Cryptovirus', which was found in 96% of CFS/ME patients tested:
    http://www.ncf-net.org/library/PressRelease0606.htm

    The article says that Dr Robbins discovered the virus (or antibodies to the virus) (in 2006?).
    It says that he tested 56 blood samples from patients with CFS/ME and found 96% of the samples tested positive for 'Cryptovirus specific antibodies'.

    He also found the same Cryptovirus antibodies in 90% of Multiple Sclerosis patients tested.

    I'm bewildered that I've never come across this research before, and that it seems to have disappeared off the radar after this press release.

    Does anyone know any more about this Cryptovirus discovery or why the research seems to have gone silent?


    Reference:
    http://www.ncf-net.org/library/PressRelease0606.htm

  2. RustyJ

    RustyJ Contaminated Cell Line 'RustyJ'

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    I also would be very interested in this, since Robbins diagnosed the virus in me. Also anyone in Brisbane who was part of Robbins study please get in touch.
  3. alex3619

    alex3619 Senior Member

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    Hi RustyJ

    I was not in the study, but I am curious to know if Dr Robbins is still in Brisbane and contactable?

    bye
    Alex
  4. RustyJ

    RustyJ Contaminated Cell Line 'RustyJ'

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    Hi Alex. No, he is in the States. The larger study referred to above was conducted in the US almost a decade after the Qld one. That is part of the problem. He left Brisbane without providing feedback to study participants. Problems with funding. There is another thread with additional info.

    Look at post #59, then #79 onwards. http://www.forums.aboutmecfs.org/sh...-Singh-8th-August/page6&highlight=cryptovirus

    Probably a good idea to start this thread rather than 'jack the other one. As I said. I am curious about symptoms of crypto... to determine if I still have any... what treatment is possible.

    There is still a chance I may not have XMRV, and that crypto is causing all the problems. I am desperate for any info. Please let me know if you get anything. Ta.

    http://www.ncf-net.org/library/PressRelease0606.htm
  5. RustyJ

    RustyJ Contaminated Cell Line 'RustyJ'

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    http://www.ncf-net.org/library/PressRelease0606.htm

    Cryptovirus may be PV5? I do have some of these symptoms.


    I found this very interesting. There is more than a suggestion that Crypto and others tie up stat-1, thus inhibiting the immune system. This seems to be a similar mechanism to how XMRV works. Hence my interest.
  6. RustyJ

    RustyJ Contaminated Cell Line 'RustyJ'

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  7. Sam Carter

    Sam Carter Guest

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    Have you tried Pubmed, RustyJ?

    A search for "parainfluenza virus type 5" retrieves 904 abstracts including 31 Reviews and 320 free, full-text papers.

    Plenty to get your teeth into!
  8. Bob

    Bob

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    Hi Rusty... like you say, it's an interesting read...

    I still can't work out what happened to Cryptovirus research, and whether Cryptovirus is thought to be the same as PIV-5, or a similar virus.
    As far as I can understand they don't appear to be saying that the two are the same thing, but very similar.

    Here's an interesting segment from the article about Cryptovirus:

  9. Dolphin

    Dolphin Senior Member

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    A quick search of PubMed for
    Cryptovirus robbins didn't show up anything.
    Nor did cryptovirus "Chronic Fatigue Syndrome".
    Was it published?

    I would like non-profits funding research to insist on publication. A lot of them do.

    I don't think the Stat-1 finding shows up on PubMed either. I think it was presented at a conference but somebody showed a reference recently and the reference authors gave was a project proposal on the NCF website:
    Reference:
    Najjar I, Deglesne PA, Schischmanoff PO, Fabre EE, Boisson-Dupuis S, Nimmerjahn F, Bornkamm GW, Dusanter-Fourt I, Fagard R.
    STAT1-dependent IgG cell-surface expression in a human B cell line derived from a STAT1-deficient patient. J Leukoc Biol. 2010 Jun;87(6):1145-52. Epub 2010 Mar 3.
  10. RustyJ

    RustyJ Contaminated Cell Line 'RustyJ'

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    CFIDS Foundation implied there was something odd going on with government databases, hints of conspiracy - hence the confusion over whether Crypto is PIV5. It appears NIH is working on creating a vaccine using PIV5 as a delivery vehicle. They say PIV5 doesn't cause problems in humans, which is quite bizarre considering all the research saying it is. This I really don't understand.

    It does appear that PIV5 fits all the symptomology of CFS. It has been found in spinal fluid.

    The issue was very big on the forums in 2006 but died away. I would like to know if there is a simple treatment. Even CFIDS Foundation seems to have gone quiet on it.
  11. Bob

    Bob

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    I can't find any more information about the Cryptovirus other than what's been posted on this thread... There isn't much info to go on, so I've done some guess work...

    I think that maybe Cryptovirus was another one of the many pathogens relating to ME/CFS that they found, probably only in a subset of people with ME/CFS, which led to a dead-end in terms of research.

    From reading the National CFIDS Foundation literature, I can't be certain but I think that Cryptovirus was either very similar to Parainfluenza Virus-5 (PIV-5), or it was actually the SER strain of PIV-5, although this is my guess work and it isn't confirmed in the literature, as far as I can see.
  12. Bob

    Bob

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    The National CFIDS Foundation (NCF) website has a long article about Parainfluenza Virus-5 and Cryptovirus and I've extracted some of the more interesting bits of info, in an attempt to make a brief summary. Taken from this webpage...

    http://www.ncf-net.org/library/PIV5HostChallenge-0606.htm

    The NCF 2006 press release about Cryptovirus, which I quoted in the opening post on this thread, is found on a different webpage...

    http://www.ncf-net.org/library/PressRelease0606.htm

    I've also extracted some bits of info regarding Cryptovirus, from this webpage:

    http://chronicfatigue.about.com/od/research/a/animalvirus.htm

    Summary:

    Parainfluenza Virus-5 (PIV-5) was previously known as Simian Virus-5, or SV5. SV5 was first isolated in 1956.

    PIV-5 is a member of the paramyxovirus family of negative-strand RNA viruses. An interesting note is that PIV-5 and measles are both from the paramyxovirus family.

    It was noticed that ME/CFS patients have a lack of Stat-1 protein in the blood, which appeared to correlate with RNaseL.

    Stat-1 degradation has been seen as a means for providing interferon antagonism by certain viruses as a method to escape the host's immune response.

    The NCF looked at which viruses were known to directly attack and degrade the Stat-1 protein.

    One specific virus which they found to deplete Stat-1 was PIV-5.

    Dr. Steven J. Robbins isolated and identified a novel RNA virus which he designated as 'Cryptovirus'. Cryptovirus is thought to be 'most similar' to PIV-5.

    A published medical journal article suggests that Cryptovirus is most similiar to Parainfluenza Virus 5, a rubulavirus in the paramyxovirus family.

    Dr. Robbins' evidence includes: Nucleotide sequence data that indicates that Cryptovirus is pandemic and represents a single virus species...

    The 'fusion protein' for Cryptovirus matched 100% with the sequence for the 'fusion protein' for the SER strain of PIV-5 (i.e. there was a significant strand of RNA identical for Cryptovirus and SER strain of PIV-5).

    NCF reported in its press release that their scientists utilized the NIH Genbank database to find the nucleotide sequence for a specific viral protein of Cryptovirus that matched 100 percent to the porcine (swine) strain of Parainfluenza Virus-5 known as the SER strain. In 1994, scientists at Bayer AG in Germany first isolated the SER strain from swine with Porcine Reproductive and Respiratory Syndrome. "This may represent a zoonotic process since zoonotic viruses are those that can be transmitted between animals and people" stated Cocchetto. The SER strain of PIV-5 had first been discovered in 1994, isolated from swine with PRRS (Porcine Reproductive and Respiratory Syndrome). The SER strain had initially been characterised as PIV-2 but was subsequently classified as PIV-5.

    Dr Robbins' research found an association between Cryptovirus and Multiple Sclerosis, Epilepsy and CFIDS. "Substantial clinical and molecular evidence indicates that this virus is involved in the development of neurological disorders that include Chronic Fatigue Syndrome (CFS), also known as Myalgic Encephalomyelitis (M.E.) by the World Health Organization, Multiple Sclerosis (M.S.) and Idiopathic Epilepsy of unknown cause."

    Dr Robbins applied to the FDA for approval for his diagnostic test for Cryptovirus, but there doesn't seem to be any further news about this.

    The NCF literature says that some strains of PIV-5 appear to cause an acute but limited illness, while other strains cause viral encephalitis and neurological disease.

    One last bit of interesting info in the NCF literature regarding the Stat-1 protein: "Since Stat-1 is responsible for type I and type II interferon responses (alpha, beta and gamma) within the cell, its role in in cellular immunity cannot be underestimated. Medical science has revealed that Stat-1 deficient cells are targets and degrades Stat-1 via one of its viral proteins known as the V protein. This V protein of PIV-5 acts to block interferon signaling. This is an important part of its viral evasion strategy against the host. Because of this strategy, the Stat-1 deficiency seen in patients with CFIDS represents an acquired host condition due to viral infection by PIV-5. Dr De Meirlier had previously identified a correlation between Stat-1 and RNaseL ratios."


    The NCF literature says:

    • "This may represent a zoonotic process since zoonotic viruses are those that can be transmitted between animals and people" stated Cocchetto.
    • Kansky commented, "Here we have what appears to be the same viral strain of Parainfluenza Virus 5 on two continents and in two different populations, swine and humans.
    • "Simply stated, it now appeared Dr Robbins' virus that he had isolated from very ill patients potentially came from swine."
    • "Here we have what appears to be the same viral strain of Parainfluenza Virus-5 on two continents and in two different populations, swine and humans." (I'm not sure what research this is refering to.)
    It seems to me that Dr Robbins was suggesting that Cryptovirus was a novel virus, but that other researchers are suggesting that Cryptovirus is very similar to the SER strain of PIV-5, or that it is the SER strain. The information seems to get a little foggy here. (The SER strain of PIV-5 was originally isolated from pigs.)

    So did Cryptovirus end up being the SER strain of PIV-5? That's not what I concluded from reading the NCF literature. Their literature says that just one protein strand of the Cryptovirus genome matched with the SER strain of PIV-5.

    __________________________________________________


    Notes:

    Rubulaviruses include: PIV5, Human parainfluenzavirus 2 (hPIV2), Mumps and Porcine Rubulavirus.

    Rubulaviruses:
    A genus of the family PARAMYXOVIRIDAE (subfamily PARAMYXOVIRINAE) where all the species have hemagglutinin and neuraminidase activities but lack a C protein. MUMPS VIRUS is the type species.

    Rubulavirus infections have been associated with encephalitis, meningitis, orchitis, inflammation of the testicles or ovaries, spontaneous abortion, and deafness, but...

    Most rubulaviruses such as PIV5, Human parainfluenzavirus 2 (hPIV2) or Mumps virus (MuV) have been shown to target one of the STAT factors for proteasome-mediated degradation.
    http://vir.sgmjournals.org/cgi/content/full/88/3/956

    Porcine Rubulavirus:
    A paramyxovirus in the genus Rubulavirus, first described in Mexico. Causes a generalized disease in pigs that includes corneal edema (blue eye) and respiratory infection. (I'm not certain, but I think that the term 'Porcine Rubulavirus' relates to Porcine Reproductive and Respiratory Syndrome, which I think is caused by the SER strain of PIV-5.)


    From Wikipedia:

    Family: Paramyxoviridae

    Subfamily Paramyxovirinae​

    Genus Morbillivirus (includes measles)​

    Genus Respirovirus (includes Human parainfluenza viruses 1 and 3, as well some common cold viruses)​

    Genus Rubulavirus (includes Mumps virus, Human parainfluenza viruses 2 and 4, Simian Parainfluenza Virus 5*)​
    [*Simian Parainfluenza Virus 5 = Simian Virus 5 = SV5 = PIV-5]​
  13. RustyJ

    RustyJ Contaminated Cell Line 'RustyJ'

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    Bob, thanks for the summary. I really struggle to interpret and focus when reading detailed information, so I appreciate your efforts. There are a lot of unanswered questions here.

    Is Stat-1 deficiency cause by crypto, or XMRV, or both? Were the symptoms attributed to crypto from crypto itself or from XMRV? Certainly the mechanism ascribed to crypto suggests it could produce the symptoms of CFS. Given that the PIV-5 variant was found both sides of the Pacific, it could be assumed that it is pervasive. If you have crypto, can you still have XMRV? Is XMRV contributing to the chronic status of crypto? I am worried about this because it appears crypto research has disappeared. At this stage I would rather have XMRV, simply because research in this area is pushing ahead.
  14. cfssufferer

    cfssufferer

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    Does anyone know what percentage the Cryptovirus/PIV-5 is found in healthy controls?
  15. Bob

    Bob

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    Hi Rusty,

    Yes, lots of questions, and little information to be found about Cryptovirus on the internet... I've no idea why the Cryptovirus trail leads to a dead end... Maybe it turned out to just be an existing strain of PIV-5 virus.

    There is plenty more information about PIV-5 on the internet, but there is far too much to even begin to make sense of it so I haven't done any searches or reading on PIV-5.

    So I'm afraid that I can only guess at some of the questions you have asked.

    All the literature that I've read about PIV-5 dates to before XMRV was discovered. I haven't heard anyone say that XMRV causes a lack of Stat-1, although I would expect that it is quite possible, as I read that there maybe a relationship between Stat-1 and RNaseL, and XMRV has been associated with RNaseL... That's how Judy Mikovits decided to look for XMRV in CFS patients in the first place... As she knew that both the prostate cancer patients, and CFS patients, had issues with RNaseL dysregulation... So maybe that means that CFS patients also have a lack of Stat-1, or Stat-1 dysregulation.

    I would guess that if XMRV turns out to be the primary infection for people with CFS, then PIV-5 and Cryptovirus might turn out to be secondary infections, just as HHV-6 and other pathogens are. I think that PIV-5 is only found in a subset of CFS patients, just like the other secondary pathogens. Cryptovirus was found in a high percentage of CFS patients, but Dr Robbins only took the samples from quite a localised population (Southeast Queensland, Australia, including the Brisbane area)... So Cryptovirus might only be found in a very localised CFS/ME patient population, who had all been exposed to the same virus due to their locality in Southeast Queensland. I remember seeing a presentation from Australian researchers who also thought that they had found another significant pathogen in local populations of ME patients. I can't remember any details though. It maybe that Australian ME patients get exposed to tropical, or exotic, pathogens (that are secondary to XMRV?) that patients in the Europe and the USA don't get exposed to.

    It might be that to be infected with both XMRV + PIV-5 (including Cryptovirus) infections cause more symptoms than XMRV alone, but that the more benign strains of PIV-5 wouldn't cause symptoms if it was the only infection. They say that some strains of PIV-5 are relatively benign, but that other strains cause complications.

    It's interesting that Dr Robbins also associated Cryptovirus with MS and Idiopathic Epilepsy, both neurological disorders. Judy Mikovits is also working on atypical MS, and autism which is another neurological disorder, and I recently heard that Andrea Whittemore, Annette Whittemore's daughter, had been suffering from Epileptic attacks and I have a friend who also suffers from Epileptic attacks, so people with ME can suffer from Epilepsy. I think that this all ties up with XMRV, and what the WPI are researching, very neatly.
  16. RustyJ

    RustyJ Contaminated Cell Line 'RustyJ'

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    Thanks Bob. I thought the researchers believed crypto was a variant of HVP-5. Also there were a lot of references to HVP-5 being developed as a vaccine vehicle - because it is believed to be harmless in humans.
  17. Bob

    Bob

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    I think I may have found the answer regarding exactly what Cryptovirus is, although it still isn't entirely clear.

    The following extracts (quoted at bottom of page) are taken from a paper published in the Journal of General Virology 2004...

    http://vir.sgmjournals.org/cgi/content/full/85/10/3007

    What they seem to be saying is that Cryptovirus is a variant of SV5 or PIV-5 (SV5 and PIV-5 are different names for the same virus).

    This publication says that Cryptovirus is a human isolate of PIV-5. SER is a swine (pig) isolate of PIV-5 and, because the Cryptovirus and SER viruses match so closely, there is speculation that Cryptovirus is a species crossover of the SER strain of PIV-5, known as a 'zoonotic process'. (Zoonotic viruses are those that can be transmitted between animals and people.)

    Was this already obvious, Rusty? Did I miss that info earlier? At least it's clearer in my own head now anyway.

    I think that the confusion is that Dr Robbins referred to his research as "the discovery of a novel human virus that has been designated as a Cryptovirus, which falls within the Rubulavirus genus of the Paramyxoviridae", but he doesn't refer to it specifically as a PIV-5 virus. http://www.wipo.int/pctdb/en/wo.jsp?KEY=02/077211&IA=US2002004117&DISPLAY=STATUS

    I'm still not sure if Cryptovirus has been officially classified as a PIV-5 virus, but it seems that maybe it's not an entirely novel virus, but possibly it's a strain of a PIV-5 virus.

    Cryptovirus definitely sits within the family of 'Paramyxoviridae' and in the genus 'Rubulavirus' which includes the following viruses: PIV-5, PIV-2, PIV-4 and Mumps.

    "...isolation of a novel human virus that has been designated as a"Cryptovirus", which falls within the genus Rubulavirus of the family Paramyxoviridae."

  18. Bob

    Bob

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    Hi Rusty,

    No, it's not a variant of HVP-5, but it seems that it might be a variant of PIV-5. The two are from entirely different families of viruses.
  19. Bob

    Bob

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    These are interesting extracts, taken from this webpage...

    http://www.wipo.int/pctdb/en/wo.jsp?KEY=02/077211&IA=US2002004117&DISPLAY=DESC

  20. Bob

    Bob

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    A good question, but I can't find the answer.

    I'm not sure if Dr Robbins actually published a paper on this work, so his work may not have been peer reviewed... Maybe this is why there is so much confusion, and so little information, surrounding this subject.

    I think that he may only have applied for a patent for a diagnostic test, and patents don't undergo the same sort of peer review of the quality of the science in the same way as a published paper does.

    I think this might be why there is so much confusion surrounding this subject... His work wasn't subjected to peer review, and he didn't have to write up his research in a format that would be acceptable for publication.

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