Ever since I found out I have MTHFR, I have been taking methylation protocol for 5 years and have experimented with various amounts of methylfolate and I do injectable mb-12 every few days. I have experimented with adding various things such as p-5-p, TMG, betaine hcl, sam-e LCF, etc. But one thing I have never experienced is the need for potassium, I have always been puzzled by this. So fast forward to lately. I really havent made much progress and felt like I was in a dead end on 2mg methylfolate and 10mg mb-12 I ran a HDRI panel and found that my folinic acid was also low and I have never thought about taking any as I got the impression it was not needed. So in addition to my 2 mg methylfolate I I had been taking. I also did a couple of other things, I added in gamma tocotrienol to protect BH4 and I have high ferritin and I have been donating blood. All of a sudden, I have had serious low potassium events. At first I just thought I was overmethylating so I took some niacin but that didnt help. Anyway, something has made a shift in my methylation block that 5 years of simplified methylation protocol hasnt addressed. So I have done 3 things total that were different, folinic acid. blood donation, gamma tocotrienol. The folinic acid does seem to be involved as I noticed it when I started taking it. They says folinic makes pyridimines and so I guess that mean nucleosides that make dna, so if I had a deficiency of folinic, then that might inhibit cell division which then would then limit the demand for potassium, no cell division, no potassium consumption. So my theory is that even though I was taking methylfolate and mb-12 which was trying to ramp up methylation cycle and cell division, there was a deficiency of nucleotides that was holding up cell division and that is why I didnt get an increased effect with taking more methylfolate. I ramped up to 5mg methylfolate and it did nothing. So the backlog theory of nucleotide shortage makes sense. I also did a organic acid urine test and the thymine didnt even register any amount. So I must have been very deficient. second, I have been studying martin pall theory and he says that peroxynitrite can destroy bh4, so I have been taking gamma tocotrienol which quenches peroxynitrite which could preserve bh4 which could then get the methylation cycle moving and then create the demand for potassium. third, I have been giving blood, I have high folic acid blood levels and I know that it can inhibit methylfolate, which can inhibit methylation cycle which can inhibit cell division. If you give blood, it will lower folic acid levels and lower the folic acid blocking effect making the methylation cycle ramp up. creating cell division and need for potassium. I gave blood yesterday and woke up with severe hypokalemia, was it giving blood lowered my electrolytes like potassium? I dont think so, because it was a startup reaction. So it must be the lowering of the blood levels of folic acid. I have been taking the gamma tocotrienol and folinic for 2 months and have experienced a little bit of low potassium, but not nearly as much as when I give blood. I am posting this to help someone out there that is stuck and is not getting their methylation block lifted as these things really havent been mentioned in trouble shooting methyation cycle blocks.