Hi all and a Happy new year! Thanks to @Daffodil I did some EliSpot tests at a lab in Germany. I never thought that I could be infected with Borellia so the results were surprising for me. Borrelia burgdorferi Elispot Borrelia burgdorferi Fully Antigen + 9 SI (< 2) Borrelia b. OSP-Mix (OSPA/OSPC/DbpA) + 11 SI (< 2) Borrelia burgdorferi LFA-1 + 3 SI (< 2) in parenthesis normal range, so seems it is positive. CMV-EliSpot + 326 SI (<2) 326 seems huge value for me! Epstein-Barr-Virus EliSpot EBV-EliSpot (lytic) + 5 SI (< 2) EBV-EliSpot (latent) + 12 SI (< 2) Coxsackie-Virus antibodies Coxsackie-Virus-IgG (IFT) + 1:3200 Titer < 1:100 Coxsackie-Virus-IgA (IFT) + 1:100 Titer < 1:10 So the comment I received from the lab was: ""it shows multiple actual infection with: CMV + EBV + Coxsackie-Virus + Borrelia burgdorferi". At that point I thought finally I have a diagnostic after about 5 years of doing hundreds of tests, Borellliosis, but I was surprised to find out that this EliSpot Lyme test is controversial. Some doctors said even I am positive on Lyme Eispot that does not mean that I was infected with Lyme or that I suffer now from Borreliosis! Some others said that I should start immediately antibiotic treatment. So I am confused, what is the final evidence that someone has Lyme? Or this test was not yet invented? Secondly, 2 years ago I did IgG and IgM for Borrelia and the result was negative! Maybe I should repeat that, but a doctor told me that IgG and IgM could be negative and I could have Lyme! So, again, very confusing. That doctor told me that I do not develop antibodies to Borellia even if I am infected! Is that possible? Then IgG and IgM are useless tests? If so, based on the same logic, one can be negative also on antibodies for HIV and still be infected with HIV? Majority of people do only antibodies tests for HIV and if that is negative then stop.