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Characterization of antibodies elicited by XMRV infection, development of immunoassay

Discussion in 'XMRV Research and Replication Studies' started by urbantravels, Aug 17, 2010.

  1. urbantravels

    urbantravels disjecta membra

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    http://www.retrovirology.com/content/7/1/68

    Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies

    etrovirology 2010, 7:68doi:10.1186/1742-4690-7-68
    Published: 17 August 2010
    Abstract (provisional)

    Background

    Xenotropic Murine Leukemia Virus-related Virus (XMRV) is a human gammaretrovirus recently identified in prostate cancer tissue and in lymphocytes of patients with chronic fatigue syndrome. To establish the etiologic role of XMRV infection in human disease requires large scale epidemiologic studies. Development of assays to detect XMRV-specific antibodies would greatly facilitate such studies. However, the nature and kinetics of the antibody response to XMRV infection have yet to be determined.
    Results

    Three rhesus macaques were infected with XMRV to determine the dynamics of the antibody responses elicited by infection with XMRV. All macaques developed antibodies to XMRV during the second week of infection, and the predominant responses were to the envelope protein gp70, transmembrane protein p15E and capsid protein p30. In general, antibody responses to gp70 and p15E appeared early with higher titers than to p30, especially in the early period of seroconversion. Antibodies to gp70, p15E and p30 persisted to 158 days and were substantially boosted by re-infection, thus, were identified as useful serologic markers. Three high-throughput prototype assays were developed using recombinant proteins to detect antibodies to these viral proteins. Both gp70 and p15E prototype assays demonstrated 100% sensitivity by detecting all Western Blot (WB) positive serial bleeds from the XMRV-infected macaques and good specificity (99.5-99.9%) with blood donors. Seroconversion sensitivity and specificity of the p30 prototype assay was 92% and 99.4% respectively.
    Conclusions

    This study provides the first demonstration of seroconversion patterns elicited by XMRV infection. The nature and kinetics of antibody responses to XMRV in primates were fully characterized. Moreover, key serologic markers useful for detection of XMRV infection were identified. Three prototype immunoassays were developed to detect XMRV-specific antibodies. These assays demonstrated good sensitivity and specificity; thus will facilitate large scale epidemiologic studies of XMRV infection in humans.
  2. shannah

    shannah Senior Member

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    Note the authors:

    Xiaoxing Qiu , Priscilla Swanson , Ka-Cheung Luk , Bailin Tu , Francois Villinger , Jaydip Das Gupta , Robert H Silverman , Eric A Klein , Sushil Devare , Gerald Schochetman and John Hackett Jr

    Retrovirology 2010, 7:68doi:10.1186/1742-4690-7-68
  3. Sam Carter

    Sam Carter Guest

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  4. Sunshine

    Sunshine Senior Member

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    I wonder if any of these assays will be made available through the upcoming WPI/UneVX lab in Nevada USA? (UneVX was VIPDX) to the general public as a 'test'?
    The XMRV antibody test is not available to us yet, although it's apparently going to be so this year or at least was....
  5. Sam Carter

    Sam Carter Guest

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    From Alter's Zagreb presentation: "XMRV and related MLVs are in the donor supply with an early prevalence estimate of 3%‐7%."

    Qiu et al in Retrovirology: "Due to the high sequence homology, the assays described herein detect antibody responses not only to XMRV but also to other known MuLVs."

    Qie et al CROI presentation: "Preliminary results showed evidence of detectable reactivity to all 3 antigens in a low proportion (~0.1%) of US blood donors."

    This shows that either the Silverman / Klein / Abbott test is under-reporting the background level of infection or Alter is over-reporting; the results differ by an order of magnitude.
  6. illsince1977

    illsince1977 A shadow of my former self

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    Characterization of antibodies elicited by XMRV infection and development of immunoas

    Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies

    Xiaoxing Qiu , Priscilla Swanson , Ka-Cheung Luk , Bailin Tu , Francois Villinger , Jaydip Das Gupta , Robert H Silverman , Eric A Klein , Sushil Devare , Gerald Schochetman and John Hackett Jr

    Retrovirology 2010, 7:68doi:10.1186/1742-4690-7-68


    Published: 17 August 2010

    Abstract (provisional)

    Background
    Xenotropic Murine Leukemia Virus-related Virus (XMRV) is a human gammaretrovirus recently identified in prostate cancer tissue and in lymphocytes of patients with chronic fatigue syndrome. To establish the etiologic role of XMRV infection in human disease requires large scale epidemiologic studies. Development of assays to detect XMRV-specific antibodies would greatly facilitate such studies. However, the nature and kinetics of the antibody response to XMRV infection have yet to be determined.

    Results
    Three rhesus macaques were infected with XMRV to determine the dynamics of the antibody responses elicited by infection with XMRV. All macaques developed antibodies to XMRV during the second week of infection, and the predominant responses were to the envelope protein gp70, transmembrane protein p15E and capsid protein p30. In general, antibody responses to gp70 and p15E appeared early with higher titers than to p30, especially in the early period of seroconversion. Antibodies to gp70, p15E and p30 persisted to 158 days and were substantially boosted by re-infection, thus, were identified as useful serologic markers. Three high-throughput prototype assays were developed using recombinant proteins to detect antibodies to these viral proteins. Both gp70 and p15E prototype assays demonstrated 100% sensitivity by detecting all Western Blot (WB) positive serial bleeds from the XMRV-infected macaques and good specificity (99.5-99.9%) with blood donors. Seroconversion sensitivity and specificity of the p30 prototype assay was 92% and 99.4% respectively.

    Conclusions
    This study provides the first demonstration of seroconversion patterns elicited by XMRV infection. The nature and kinetics of antibody responses to XMRV in primates were fully characterized. Moreover, key serologic markers useful for detection of XMRV infection were identified. Three prototype immunoassays were developed to detect XMRV-specific antibodies. These assays demonstrated good sensitivity and specificity; thus will facilitate large scale epidemiologic studies of XMRV infection in humans.​

    Dare I hope that the ball is finally rolling and our time has come at last?
    :victory::D:tear: (tears of joy!!!) :D:victory:
  7. Otis

    Otis Señor Mumbler

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    That looks great. The team of Silverman & Klein are back together which adds instant credibility.
  8. urbantravels

    urbantravels disjecta membra

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  9. ggingues

    ggingues $10 gift code at iHerb GAS343 of $40

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    Who you calling a Germ, Urbanite?

    130 posts and you can access the Library!

    FYI
  10. urbantravels

    urbantravels disjecta membra

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    Well, I must be a germ too...since I'm certainly no lady :Retro wink:
  11. John Leslie

    John Leslie

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    I heard that VIPdx will announce that its serology tests will be available before next week. Their tests will be more specific than Abbott's because they look for more than just VP62; you get all the variants with the WPI licensed tests.

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