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cell permeability, mitochondria since I am lost and lost hope---------------

Discussion in 'ME/CFS Doctors' started by ninauae, Nov 23, 2012.

  1. ninauae

    ninauae

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    I am waiting for my genetic test result from Dr Amy Yasko which will come in Feb 2013. In that mean time if some one can help me with my symptoms to solve my problem. Sorry their are two many tests this year and its long story.

    Personal Info:

    Female; 36 years old; work as an house wife; Walk daily 45 min; non‐smoker;
    History:
    • I am on 700 kcal diet since long time but cannot lose weight. I have anemia problem after my first pregnancy and recently started taking iron since sulfur gives me breathing problem.
    • My spo2 is always 98 – 100% but when its 100% give me breathing problem because of oxygen toxicity.
    • Last year in April 2011 my breathing problem got worse with both nasal turbinate swelling because that time taking oats for my breakfast plus All Bran cereal and pineapple extract with normal milk.
    • I have high CRP(C - reactive protein) with high ESR normal is 20 but mine was 40 last year. This year in 0ct 2012 IL-6 and IL -10 cytokines are in higher range as well.
    • I cannot lose weight recently thought because of high thiol (sulfur) and phenols in diet. I diagnose this in my Amino Acid test and Hair Anylasis results this year. Matter of fact never took calcium supplement or drank milk in my entire life.
    • I have high bone mineral density in my lower spine and its +3 through Dexa bone scan.

    • Last month did my serum b12 test which showed high b12 value but in matter of fact at urine amino acid test it is in lower range.
    • My IGE values were 94 last year and metal binding capacity is low. I did delayed food sensitivities ALCAT Test which identifies I have allergies toward 70 food items.
    • I think maybe I am TH2 dominant because last year my Vitamin D status was 22 after taking supplementation. My Amylase lipase test results are in normal range. My urine morning PH is always 6 and liver test were normal in 2010.

    Symptoms:

    1. My both nose turbinate are large and swollen.
    2. I cannot lose weight and when eat allergies food cannot travel because of breathing problem with mild asthma symptoms.
    3. My belly button waist measurement is 38 inches with upper back hyper kyphosis because of no calcium intake due to high oxalate found in amino acid test. I have total of 41% of body fat which is more in belly.
    4. I have low energy and when feeling low take 1000mg sublingual b12 after 3 days.
    5. I cannot drink more than one glass of water in day because high ADH hormone and the cause are unknown. The doctor doesn’t feel concern since my 24 hrs urine sodium and chloride are in normal range. My blood Sodium is 143 and matter of fact never took salt a day nor high salt diet.

    6. My Serum copper and zinc both were high last month along with b12. Folic acid was low normal range as sometimes I think it might be because of methylation issues.
    7. Mineral bio unavailability issues as I am not sure of it. My rt3 values were higher earlier this year.
    8. I have this inflammation problem from long time.

    After reviewing my problem I came to following conclusion:
    Serum hormones blood
    Urine hormones compare results – blood capillary hormones
    1. Thyroid serum T4 in upper highrange------------ Thyriod Blood spotresult in lower range
    2. Carnitine in blood low ------Carnitine in urine toward eleveted
    3. Serum blood Copper levels are high --------- Low Copper levels in hair analysis(intra cellular)
    4. Serum B 12 levels are high ---------- Urine Amino Acid methylmalonic acid -B 12 is low
    5. Zinc serum high but hair anylasis Zinc level are in range
    6. blood Iron middle is in range but blood Ferritin is in low site – low iron in hair anylasis
    7. Calcium serum ok------------High Calcium in hair anylasis but low in 24 hrs urine anylasis.

    Matter of fact never took Cal my entire life + Magnesium
    8. RBC Folic Acid - low
    9. Serum Magnesium ok ---------------Magnesium hair analysis toward high
    10. Serum Cortisol toward high -----------------Cortisol urine ok
    11. Anti diuretic hormone high
    12. Spo2 99% Urine Ph usually 5.5 to 6
    Only these few hormones match their serum and urine results are Aldosterone is low and Anti body thyroid hormone is ok. Sometimes I think their is problem with cell memebrane or cell permeability. It can also be from PST enzyme deficincy since sulfur is problem for me.
    Why my serum Zinc, Copper are in high normal range and same time intracellular Molydbenum is elevated at HA test?
    My Doubts
    I think after comparing these results may be my problem cause is:
    1. Cellular Signaling Problem
    2. Slow 8 detox pathway recently showed in hair analysis report result this month. Plus I have adrenal fatigue and secondary reason is slow thyroid hormones.
    3. Glutamate problem which got spotted as calcium deposit in spinal cord tissues = high bone mineral density and fact is never took calcium and magnesium in my life as following 700 Kcal diet from ages. This protocol described at Dr Amy Yasko. But my amino acid Glutamine is in lower range.
    4. My heart beat changes every second and usually it’s in high range 95 beats per minute and my breath rate count at rest are 21breath per minute.
    5. Severe inflammation problem since my belly button is 42 inches and total body fat is 42% (mostly around belly).
    6. Th1/Th2 ratio imbalance since my cellular glutathione and serotonin very low with Vit C and Vit D at 22.
    7. I have problem with sulfur since last year I tried garlic supplement which made my breathing problems worse. My spirometry result revealed Asthma is at border line. I experience shortness of breath while walking sometimes and my spine hurts since this year curvation of spine and hyperkyphosis also started may be because of not taking calcium at all. 8. My total water intake is 1 glass a day since more give my belly bloating and feeling full quickly after meal. My food intake at a time is usually 3 cups if I consume more than bloat feeling. I checked my Gastric Acid and mine was .60 and normal range is less than 1.
    9. My nose cartilage has grown since PST issues. My urine amino acid test Arginine and Lysine was in normal high range. I have all body inflammation problem including my both nasal turbinate’s are large and swell.
    I think My MT proteins are not working properly and ceruloplasmin might also be low since Ferritin is in lower side.
    10. I feel low energy with lot of chronic fatigue symptoms. This all because of my cellular signaling and detox pathway are slow esp. G protein pathway and DPP iv deficiency or excess .
    11. I am waiting for my results from Dr Amy Yasko methylation panel and sometimes I think I have PST and MTHFR gene problem but not sure of it.
    Meds and Supplements:
    1. Started Vit C 500, Q 10 50 mg
    2. I probiotics and night time I dandelion capsule which made my abdomen swelling worse
    Major Medical Events:
    I had 3 C section deliveries.
    In Conclusion:
    There is no doubt in my mind that I suffer from allergies weight issues because:
    The symptoms support this.
    Doctors think I have mal absorption problem because of low good intestinal flora so recommending following Paleo diet.
    My symptoms got improved from last year to avoid thiol foods and introduce b12 tablet in my diet after every 3 days.
    Please advise me for this issue and make diet chart according to my thiol and phenols issues. I have cellular signaling problem with slow 8 body detox pathway eliminators. This month Dr Wilson of hair analysis report that I have slow metabolism with adrenal Fatigue and slow thyroid function. I am not following his diet because more I eat than my normal routine as severe throat swelling problem with abdomen distention.
    I didn’t start chelating since my cellular Glutathione and Vitamin D levels are very low so it made me worse before I start any program.
    LeMeridian Valley lab result
    19th Jan 2012-------blood serum Thyroid
    Thyroid Antibody (ATA) 13.2 0 – 35-- ref range
    Anti- TG 31.6 0 - 40
    Reverse t3 30.7 6 - 21.8
    F T 3 3.6 1.8 - 3.9
    F T 4 0.91 0.89 - 1.7
    TSH 2.3 0.25 - 3.5
    ZRT Lab
    Thyroid blood spot on 6th Dec 2011
    Iodine (Urine) 263 ug/L 100 1100 ug/L
    (150 – 300) optimal
    Creatinine 2.7 mg/ml 0.3 3.5 mg/ml
    Iodine/creatinine 97 L ng/ml 100-300 ug/g creatinine
    Thyroglobulin (blood spot) 5 ng/dl 3 – 40
    3 – 10 optimal
    Total T4 (blood spot) 11 ng/dl 5-10.8 ug/dL
    Free T4 (blood spot) 2 uu/ml 0.7 – 2.5
    Free T3 (blood spot) 2.6 pg/ml 2.5-6.5
    TSH (blood Spot) 2.6 uu/ml 0.5 - 3
    TPO blood spot 17 0-150 (70-150 borderline)
    19th Dec 2011
    OAT – Organic Acid Test Urine test
    Yeast and Fungal Markers
    Citramalic ≤ 3.6---------------0.52
    5- Hydroxymethyl-2-furoic ≤ 14---------3.5
    3-Oxoglutaric ≤ 0.33--------------0.07
    Furan-2,5-dicarboxylic ≤ 16-----------4.5
    Furancarbonylglycine ≤ 1.9------------- 0.10
    Tartaric ≤ 4.5 -----------0.72
    Arabinose ≤ 29------------ H 39
    Carboxycitric ≤ 29 -------------0.19
    Malabsorption and Bacterial Markers
    2-Hydroxyphenylacetic 0.06 - 0.66 ---------------0.21
    4-Hydroxyphenylacetic ≤ 19------------- 5.4
    4-Hydroxybenzoic ≤ 1.3----------------- 0.20
    4-Hydroxyhippuric 0.79 - 17 ---------------6.0
    Hippuric ≤ 613---------------- 119
    3-Indoleacetic ≤ 11------------ 0.40
    Succinic ≤ 9.3 ------------1.2
    HPHPA (Clostridia marker) ≤ 208 ----------------10
    DHPPA (Beneficial bacteria) ≤ 0.38 ------------0.04
    Oxalates Metabolites
    Glyceric 0.77 - 7.0------------ 6.7
    Glycolic 16 – 117---------- 30
    Oxalic 6.8 – 101---------------- 29
    Glycolytic Cycle Metabolites
    Lactic ≤ 48--------- 10
    Pyruvic ≤ 9.1 ------------4.1
    2-Hydroxybutyric 0.03 - 1.8 -----------0.42
    Kerbs Cycle Metabolites
    Succinic ≤ 9.3 ---------1.2
    Fumaric ≤ 0.94 ------------0.13
    Malic 0.06 - 1.8 ---------0.14
    2-Oxoglutaric ≤ 35 -----------5.3
    Aconitic 6.8 - 28 ----------L 6.1
    Citric ≤ 507 ---------------59
    Neurotransmitters Metabolites
    Homovanillic (HVA) - Dopamine- 0.80 - 3.6------------- 1.1
    Vanillylmandelic (VMA) ---------- (norepinephrine, epinephrine) 0.46 - 3.7---------- 0.88
    HVA/VMA Ratio 0.16 - 1.8 ------------1.2
    5-Hydroxyindoleacetic (5-HIAA) Serotonin--------- ≤ 4.3---------- 0.22
    Quinolinic 0.85 - 3.9 ---------------1.2
    Kynurenic 0.17 - 2.2 ------------1.1
    Quinolinic/5-HIAA Ratio 0.42 - 2.0 ---------------H 5.2
    Pyrimidiness - folate Metabolism
    Uracil ≤ 9.7 ------------2.3
    Thymine ≤ 0.56 ------------0.06
    Ketone and Fatty acid oxidation
    3-Hydroxybutyric ≤ 3.1 -----------0.52
    Acetoacetic ≤ 10------------- 2.1
    4-Hydroxybutyric ≤ 4.8 ----------1.1
    Ethylmalonic 0.44 - 2.8 ------1.2
    Methylsuccinic 0.10 - 2.2 ---------0.72
    Adipic 0.04 - 3.8 ---------0.78
    Suberic 0.18 - 2.2 ------------H 2.9
    Sebacic ≤ 0.24 -----------0.01
    Nutritional Markers
    Vitamin B12
    Methylmalonic ≤ 2.3----------- 0.22
    Vitamin B6
    Pyridoxic (B6) ≤ 34 -------------0.48
    Vitamin B5
    Pantothenic (B5) ≤ 10 ----------1.0
    Vitamin B2 (Riboflavin)
    Glutaric 0.04 - 0.36 ---------0.14
    Vitamin C
    Ascorbic 10 – 200----------- L 0.64
    Vitamin Q10 (CoQ10)
    3-Hydroxy-3-methylglutaric 0.17 - 39 ------------3.8
    Glutathione Precursor and Chelating Agent
    N-Acetylcysteine (NAC) ≤ 0.28----------- 0.02
    Biotin (Vitamin H)
    54 Methylcitric 0.19 - 2.7------------- 0.37
    Indicators of Detoxification
    Pyroglutamic 10 – 33---------- L 7.8
    Orotic 0.06 - 0.54----------- 0.16
    2-Hydroxyhippuric ≤ 1.3-------------- 0.33
    Amino Acid Metabolites
    2-Hydroxyisovaleric ≤ 0.42--------- 0
    2-Oxoisovaleric ≤ 2.1 ---------0
    3-Methyl-2-oxovaleric ≤ 0.87 ---------0
    2-Hydroxyisocaproic ≤ 0.48----------- 0.02
    2-Oxoisocaproic ≤ 0.37---------- 0.08
    2-Oxo-4-methiolbutyric ≤ 0.16 -----------0.05
    Mandelic ≤ 0.21----------- 0.04
    Phenyllactic ≤ 0.20 ----------0.02
    Phenylpyruvic 0.20 - 1.9------------- 0.32
    Homogentisic ≤ 0.36-------------- 0.01
    4-Hydroxyphenyllactic ≤ 0.80 ----------0.20
    N-Acetylaspartic ≤ 3.0 ----------0.19
    Malonic ≤ 9.7----------- 6.9
    3-Methylglutaric ≤ 0.76------------ 0.13
    Bone Metabolites
    Phosphoric 1 000 - 5 000 ---------------1715
    Amino Acid Urine
    Creatinine 180 mg/dL 35-- 225
    Glutamine/Glutamate 18 5 - 160
    Ammonia Level (NH4) 25600 μM/g 12000- 49000
    ESSENTIAL / CONDIIONALLY INDISPENSABLE AMINO ACIDS
    Methionine 8.6 8- 48
    Lysine 400 40- 530
    Threonine 95 75- 330
    Leucine 21 22- 100
    Isoleucine 6.8 9- 50
    Valine 27 15- 70
    Phenylalanine 35 25- 100
    Tryptophan 39 20- 100
    Taurine 91 220- 1300
    Cysteine 29 25- 73
    Arginine 31 8- 55
    Histidine 560 350- 1700
    Nonessential Amino Acid
    Alanine 190 130- 600
    Aspartate 4.8 8- 30
    Asparagine 46 35- 200
    Glutamine 200 200- 740
    Glutamate 11 6- 52
    Cystine 87 30- 105
    Glycine 790 500- 4100
    Tyrosine 69 28- 120
    Serine 140 180- 600
    Proline 3.6 1- 55
    Gastrointestinal Markers
    Ammonia (NH4) 25600 12000- 49000
    Ethanolamine 190 120- 440
    Alpha-Aminoadipitate 13 6- 72
    Threonine 95 75- 330
    Tryptophan 39 20- 100
    Taurine 91 220- 1300
    Beta-alanine 1.8 < 20
    Beta-aminoisobutyrate 35 < 380
    Anserine 9.4 < 95
    Carnosine 5 < 50
    Gamma-aminobutyrate 1.1 < 15
    Hydroxyproline 0.88 < 45NTESTINAL MARKERSENTIA
    MMAGNESIUM DEPENDANT MARKERS
    Magnesium Dependent Markers
    Citrulline 3.7 1- 30
    Ethanolamine 190 120- 440
    Phosphoethanolamine 18 20- 75
    Phosphoserine 0.057 0.05- 0.8
    Serine 140 180- 600
    Taurine 91 220- 1300
    68th 95th
    Methionine Sulfoxide 1.4 < 10
    B6, B12, & Folate Depended Markers
    & FOLATE DEPENDANT MARKERS
    Serine 140 180- 600
    Alpha-aminoadipate 13 6- 72
    Cysteine 29 25- 73
    Cystathionine 14 8- 50
    1-Methylhistidine 180 70- 280
    3-Methylhistidine 710 55- 1100
    Alpha-amino-N-butyrate 8.8 5- 72
    68th 95th
    Beta-aminoisobutyrate 35 < 380
    Beta-alanine 1.8 < 20
    Homocystine 0.12 < 5
    Sarcosine 1.9 < 50B12, & FOLATE DEPENDANT MARKERS
    Detoxification Markers
    Methionine 8.6 8- 48
    Cysteine 29 25- 73
    Taurine 91 220- 1300
    Glutamine 200 200- 740
    Glycine 790 500- 4100
    Aspartate 4.8 8- 30
    Neurological Markers
    Ammonia (NH4) 25600 12000- 49000
    Glutamine 200 200- 740
    Phenylalanine 35 25- 100
    Tyrosine 69 28- 120
    Tryptophan 39 20- 100
    Taurine 91 220- 1300
    Cystathionine 14 8- 50
    68th 95th
    Beta-alanine 1.8 < 20
    Urea cycle Metabolites
    Arginine 31 μM/g 8- 55
    Aspartate 4.8 μM/g 8- 30
    Citrulline 3.7 μM/g 1- 30
    Ornithine 13 μM/g 3- 45
    Urea 250 mM/g 150- 590
    Ammonia (NH4) 25600 μM/g 12000- 49000
    Glutamine 200 μM/g 200- 740 68th Percentile
    Asparagine 46 μM/g 35- 200 
    CYTOKINES, Extensive Panel – Blood spot test on 25th Oct 2012
    Age diagnostic lab USA
    iINFg 9.3 0.0 - 16.0 pg/mL
    Interleukin 1 2.1 0.0 - 5.0
    Interleukin 2 19.6 0.0 - 32.0
    TNFb 51.4 0.0 - 156.0
    Interleukin 4 22.7 0.0 - 32.0
    Interleukin 5 6.9 0.0 - 8.0
    Interleukin 6-----------8.8 *H----- 0.0 - 8.0
    Interleukin 10--------- 9.3 *H----- 0.0 - 8.0
    Interleukin 13 2.8 0.0 - 8.0
    TNFa 0.74 0.00 - 1.00
    TGFb 38.0 28.0 - 64.0
    On 13th October 2012 I did Venous Blood gases (Cellular Blood gases) test and the results are following:
    VBG (Venous Blood Gases) 13-Oct 2012
    Body temperature intracellular 37C
    VPH 7.294 7.31 – 7.41
    VpCo2 37.6 mmHg 40-60 mmHg
    VpO2 28.2 mmHg 30-55 mmHg
    FIO2 % 20.9 Hb 14.3
    BEecf - 8.3
    BEb - 6.9
    Sbc 17.9
    HCo3 18.4 mmol/L 22 – 27 mEq/L
    TCO2 19.6
    So2 46.9 % 40-85%
    At the same time my pulse oximeter oxygen was 99% so I searched on the net and its look like mix acid base disorder according to my VBG result. I think its metabolic acidosis and also respiratory acidosis as well.
    I think my intracellular and extracellular oxygen transport patterns are not well balanced as well.
    So let put things all together and the following are my major issues.
    1. Cell communication problem
    2. Its block methylation since amino acid test and my glutathione level indicate it.
    3. Bio unavailable minerals even blood gases have different report values and oxygen transport problems.
    4. Detox pathway (liver 1 and 2) are not working problem this month hair analysis report results.
    5. Urea Bun Nitrogen levels are always low. I hardly sweat from ages so its underactive sweat glands as well.
    6. Bone mineral deposit because of NF – Kappa B ( osteoblast and osteoclast imbalance)
    7. Th1/Th2 might be imbalance since my Vit D was 22.
    I have more body warts and large nasal turbinate’s with bulbous nose as compare to before.
    Please guide me in able to uplift block methylation safely what are the steps which will not make my breathing inflammation worse.
    I want to start everything slowly and want to drink more than a glasses of water in a day. Sometimes thinking I have rennin – angiotensin issues but results are in normal range so it might be because of cellular communication and block methylation issues.
    Hair analysis – Cellular Minerals 18th Sep 2012
    Calcium 83 28 - 81
    Magnesium 9 4 - 9
    Sodium 12 L 13 - 45
    Potassium 2 L 5 - 18
    Iron 0.7 L 2 – 4.5
    Copper 0.9 L 1.5 - 3
    Manganese .007 L 0.02 – 0.0006
    Zinc 17 ok 16 - 26
    Chromium 0.081 L .008 – 0.16
    Selenium 0.061 L 0.12 0.21
    Phosphorus 12 L 12.8 - 19.2
    Nickel .009 Less than 0.03
    Cobalt .001 Same as above
    Molybdenum .005 H Same as above
    Boron zero
    Lithium .003 Ok Low 0.1 0.3[​IMG]FREE 003 Ok Low 0.1 0.3end_of_the_skype_highlighting
    9th Jun 2012 all urine samples 24 hrs hormones report
    Potassium ( 24-hrs urine) 55.20 mmol/L 25 - 125
    Calcium 94.8 L mg / 24 hrs 100 - 300
    Sodium 178 m Eq/ day 40 - 220
    Chloride 178 110 - 250
    Creatinine 1226 mg/24 hrs 500 - 2000
    Amylase 160 U/L 32 - 641
    Zinc 14 2.8 - 13
    copper 10 Less than 60
    5th May 2012 (Blood sample)
    A1C 5.7 4 - 6
    Calcium 9.5 8.7 – 10.2
    Ferritin 17 5 - 204
    % iron Saturation 19 15 - 50
    iron 75 37 - 181
    Total iron binding capacity 391 260 - 400
    Gastric Acid 0.6 Should be less than 1
    ADH - fasting 3.4 Up till 1.7
    15 – Aug – 2012 (All Blood sample)
    Vitamin B 12 (serum) 860 -----------------(211 - 946)
    Blood urea Nitrogen (BUN) 10--------(8 - 22)
    Sodium - Serum 143--------136 - 145
    Potassium - Serum 4.4 ------3.5 – 5.4
    Magnesium - Serum 1.9 1.5 – 2.6

    Chloride - serum 102 -----95 - 108 Copper - serum 156 ---------------80 - 160
    Red Cell Folic Acid 167 --------------------------(93 - 640)
    Zinc - serum 25 ---------------10.7 – 17.5
    CRP (high sensitive) 4.8 -----Up to 5
    Progesterone (1st day) 0.28 -----------0.2 – 1.5
    Estradiol (1st day) 45.6----------- 12.5 - 166
    Cortisol - PM 210 ------------64 - 327
    Hemoglobin (Hb) 11.8 11.1 - 14.5
    Total RBC 4.2 ---------3.9 - 5.5
    HCT 40 --------35 - 42
    MCV 93---------- 75 - 95
    MCH 28------ 26 - 32
    MCHC 30 L -----------32 - 36
    Platelet Count 258---------- 150 - 400
    WBC Count (TLC) 8 --------4 - 11
    Neutrophils 69 ---------40 - 75
    Lymphocytes 25 --------20 - 45
    Monocytes 4 -------2 - 10
    Eosinophils 2 --------1 - 6
    Thank you for reviewing my all history sonce my Aldosterone was in lower range in 2010 as well this year alos its low in urine test so may be its link to estogen deficincy as well. Last month my IL- 6 cytokine is in higher range which can cause from estogen or DHEA deficincy as well.
     
  2. SanDiego#1

    SanDiego#1 SanDiego#1

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    SouthEast USA

    Nan - How long have you been seeing Dr. Yasko? Any improvement in symptoms? Would really like to see someone on this site give you a good response. Sounds like you have had every test imaginaable. Although I have had many of the same tests you had am not qualified to give you a good answer. There are people on this site that can.. If you don't improve-You may need to seek another Physician. Please don't give up- There is someone out there that can help you. Please let me know what happens.

    Much care to you.

    San Diego#1
     

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