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Blood Products Advisory Committee Meeting Background Material

Discussion in 'XMRV Testing, Treatment and Transmission' started by ixchelkali, Jul 15, 2010.

  1. Eric Johnson from I&I

    Eric Johnson from I&I Senior Member

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    It was boring, mostly. I thought Dr Le Grice was impressive. Because of what he's choosing to focus on, and also because of his very deliberate attitude.
  2. alex3619

    alex3619 Senior Member

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    Logan, Queensland, Australia
    Hi,

    I don't recall reading that this was confirmed on actual patients. They might be able to find the usual XMRV, but can they find it in us? They were looking for standard nucleic acids, not modified by the immune system.

    Bye
    Alex

  3. muffin

    muffin Senior Member

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    Thank you for going and sitting there and taking notes. Don't you just love Gaithersburg?

    "The worst part of Dr. Hendrys presentation for me was after it was over. We are used to the lying, the manipulation and the sheer audacity to give false information as though they are facts. But, when asked by Dr. Hollinger if the PCR gag was the same as the Lombardi study, he said Yes. A bold faced lie. Its one thing to read about these moments, but its quite another to see the lying in person, not to mention the slight swagger of Dr. Hendry as he walked back to his seat." THIS MUST HAVE MADE YOU CRAZY. HOPE THIS GUY GETS A GOOD SMACK IN THE BUTT WHEN IT ALL COMES OUT. What the heck is with these peope?????
  4. George

    George waitin' fer rabbits

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    You would be correct, they tested blood samples from patients provided by the WPI and spiked to differing degrees. Butttttt. . . they look to be in "validation" or pre- production mode based on the article from Medsacape.

  5. Lynn

    Lynn Senior Member

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    I have the test kit from VIP dx and am waiting to get my blood drawn until they have the serology test available (in the next 10 days). I am now wondering if I should wait to get tested. Maybe a validated test is just around the corner. I am afraid that if I only have the VIP dx test my insurance won't cover ARV therapy. Will I just be throwing $250 down the drain in my efforts to validate my illness?

    Lynn
  6. SOC

    SOC Moderator and Senior Member

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    I've got two VIPdx test kits coming. I'm wondering the same thing... should I wait?
  7. Sushi

    Sushi Moderator and Senior Member Albuquerque

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    Me too! I called VIP yesterday and they said that they hoped to have the test available by the end of the week. My test kit should arrive any day.....

    What do others think?

    Sushi
  8. George

    George waitin' fer rabbits

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    Only 250$ for an XMRV test????

    New thread anyone?????
  9. Rrrr

    Rrrr Senior Member

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    george, where did this below quote come from???



    "The HIV single copy that was developed at the NCI is regarded as the gold standard assay. We now believe that we have an equivalent assay for XMRV."

    Dr. Le Grice and his team have also developed a cell line, dubbed the Derse cell line, which can detect XMRV in as little as 3 days.

    He said that the XMRV assay that his lab has developed has been transferred to labs in Sweden, Australia, Vietnam, and South Africa to prove its utility. "Developing an assay is one thing, but transferring it to a laboratory where it can be reproduced is clearly important when we are talking about single copy assay. Contamination is a huge problem, and the ability to transfer these reagents is very important," he noted.

    Dr. Le Grice added that the aim of the NCI is to make sure that the assays they have developed are as valid as possible. "Our goal is to develop a series of assays that we feel confident in and to test those head to head with other assays. I think that is really important at the moment. We should start with 6 assays in house, and if we have a problem, I think it is important to sit amongst ourselves and try to understand where those problems are before we disagree with anybody else's assay."
  10. Rrrr

    Rrrr Senior Member

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    cdc

    so the cdc still says they can not find xmrv? i read on another thread (where?) that they now can find it. but at this FDA meeting they only reported that they can not find it?
  11. guest

    guest Guest

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    I'm hoping so much that you are right with this.
  12. anciendaze

    anciendaze Senior Member

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    Here's a possible interpretation. They found XMRV in about 1% of tissue samples from prostate cancers. This was reported at a conference months ago. It looks like this allows them to say: 1) yes, we know how to find XMRV; 2) no, it is not in the blood supply. Had they failed to find it anywhere, they would have lost credibility. In this case, it seems the test assay was improved just enough to satisfy policy objectives.

    I doubt we are out of the woods w.r.t. testing. Evidence for hypermutation, to say nothing of possible recombination, means finding specific sequences may be considerably more difficult than anticipated. If, as I suspect, most sequences were mutated prior to collection, the role of activation and biological amplification could be more important than simply increasing the number of copies.

    If activation caused the virus to insert new copies of its genes, these would not be immediately subject to mutation. Assume, for the moment, biological amplification goes through four generations. If the sequences were already present, you might see a change from a single copy to 16. In this case, chance alone would suggest finding some XMRV, not 0%. On the other hand, if all preexisting inserted sequences were mutated, you might see a difference between zero copies and 16.

    This idea may well be wrong, but it fits published results pretty well.
  13. Megan

    Megan Senior Member

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    I have been wondering about this too. It will be interesting to see what the Alter paper says on the CFS patients, whether they have XMRV or other MLVs? From the press leak it sounded like he was saying that there are other MLV's involved. If this means other MLVs than XMRV, then we could be waiting a long time for the testing to be sorted out - we may all have different MLV's. That would mean lots of different tests.
  14. George

    George waitin' fer rabbits

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    Happy Note (well sort of)

    Hey ya guys
    When I started researching MLV's back in January I noticed an interesting phenomena. Two seperate papers from 1984 and 1987 were "republished" both papers were on the 'clearance of MLV's from human and primate systems within 10 days. MLV's themselves have passed the test of 40 years of use and testing in humans as essentially harmless.

    Fast forward to Dr. Sandra Ruscetti (and Frank and Dr. Coffin and Dr. Goff and many others) Who have been studying Xenotropic - MLV - retroviruses in "other" mammals for over 20 years. One of the main features that were found was that many of these species of MLV's "recombined" with other MLV's to get by the animals defenses. But a base MLV was observed in all cases. In other words SFFV might recombine with other MLV's but the SFFV was the base virus needed in order for the other MLV to recombine.

    So test us for XMRV, if we have XMRV, treat for the XMRV retrovirus. The other MLV's will clear from the system. Course this is the simplest version, there's still secondary infections ,problems with IRIS, medication side effects and all that good stuff. But hey it will be a start.
  15. markmc20001

    markmc20001 Guest

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    CDC

    Am I understanding correctly that the CDC claims to have found XMRV in only 1% of prostate cancer tissues? Does that sound kind of like the CDC is giving the same XMRV denial treatment to prostate cancer folks?

    if that is the case....yikes.

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