Invest in ME Conference 12: First Class in Every Way
OverTheHills wraps up our series of articles on this year's 12th Invest in ME International Conference (IIMEC12) in London with some reflections on her experience as a patient attending the conference for the first time.
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Bioinformatics collaboration

Discussion in 'Other Health News and Research' started by undiagnosed, Jun 16, 2017.

  1. undiagnosed

    undiagnosed Senior Member

    United States

    I want to revive a project I started working on to do nucleic acid amplification primarily for the purposes of pathogen detection and discovery with minimal equipment requirements using a Loop-Mediated Isothermal Amplification (LAMP) based assay. I am looking for anyone with bioinformatics experience to help by providing peer review and advice. My interest is in lentiviruses, but we could do other pathogens as well. I am currently working on designing primers, but it's my first time so I am trying to figure out sequence alignment and using tools like re-pcr and e-lamp to determine primer specificity in-silico prior to getting the primers manufactured.

    Here is a thread I started a while ago which has some initial research I did. It was more focused on detecting reverse transcriptase activity but still using a LAMP assay.

    I also have a few documents started including, RT-LAMP assay for detecting lentiviruses and A minimal equipment one-pot RT-LAMP assay for detecting reverse transcriptase activity.

    Let me know if you are interested in helping out or know anyone who may be.

    Thank you
    Last edited: Jun 17, 2017

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