I worked up gradually to my current doses over a 6 month period. Currently take 37.5 mg MB12 by injection daily in 3 divided doses with more in the earlier part of the day. 18.75 mg folate (Deplin). I have been taking 6 ADB12 daily (Source Naturals or Anabol) but have recently been able to cut that back to 1-3 daily and am doing okay. When I started the protocol I was already taking MB12 but only 5 mg twice a week by injection. I had to get the folate way up before I could add more B12. I started using OTC folate but when I had to take so many I asked for a script and switched to Deplin. I improved noticeably after that switch.