Omnerbasket, You seem to think that calibrating a PCR test is not enough to ensure that the test will find the sequence it was calibrated for. I really don't think the argument you are using works well, particularly because WPI used the same calibration target as most of the other tests. You seem to be suggesting that a PCR test, designed by WPI, which is calibrated to VP62, is somehow more reliable than a test designed by another lab, also calibrated to VP62, if they do not have the same finding. Who made WPI the reference here? Their findings are just as subject to confirmation as anyone else's. And most of their secondary testing rests on the validity of their original PCR test. So this IS a major issue. If there is a difference in two PCR tests calibrated to the same sequence, either one of them is wrong (unlikely), or one of them has sample differences (very likely). What makes those samples different is probably the primary mystery to solve.