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Aged Garlic Extract and Its FruArg Induced Neuroinflammatory Response in Microglial Cells

Discussion in 'Other Health News and Research' started by Ecoclimber, Nov 26, 2014.

  1. Ecoclimber

    Ecoclimber Senior Member

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    PLoS One. 2014 Nov 24;9(11):e113531. doi: 10.1371/journal.pone.0113531. eCollection 2014.
    Proteomic Analysis of the Effects of Aged Garlic Extract and Its FruArg Component on Lipopolysaccharide-Induced Neuroinflammatory Response in Microglial Cells.
    Zhou H1, Qu Z1, Mossine VV2, Nknolise DL1, Li J3, Chen Z1, Cheng J3, Greenlief CM4, Mawhinney TP2, Brown PN5, Fritsche KL6, Hannink M2, Lubahn DB2, Sun GY7, Gu Z8.

    Abstract
    Aged garlic extract (AGE) is widely used as a dietary supplement, and is claimed to promote human health through anti-oxidant/anti-inflammatory activities with hypolipidemic, antiplatelet and neuroprotective effects.

    Prior studies of AGE have mainly focused on its organosulfur compounds, with little attention paid to its carbohydrate derivatives, such as N-α-(1-deoxy-D-fructos-1-yl)-L-arginine (FruArg).

    The goal of this study is to investigate actions of AGE and FruArg on antioxidative and neuroinflammatory responses in lipopolysaccharide (LPS)-activated murine BV-2 microglial cells using a proteomic approach.

    Our data show that both AGE and FruArg can significantly inhibit LPS-induced nitric oxide (NO) production in BV-2 cells.

    Quantitative proteomic analysis by combining two dimensional differential in-gel electrophoresis (2D-DIGE) with mass spectrometry revealed that expressions of 26 proteins were significantly altered upon LPS exposure, while levels of 20 and 21 proteins exhibited significant changes in response to AGE and FruArg treatments, respectively, in LPS-stimulated BV-2 cells.

    Notably, approximate 78% of the proteins responding to AGE and FruArg treatments are in common, suggesting that FruArg is a major active component of AGE. MULTICOM-PDCN and Ingenuity Pathway Analyses indicate that the proteins differentially affected by treatment with AGE and FruArg are involved in inflammatory responses and the Nrf2-mediated oxidative stress response.

    Collectively, these results suggest that AGE and FruArg attenuate neuroinflammatory responses and promote resilience in LPS-activated BV-2 cells by suppressing NO production and by regulating expression of multiple protein targets associated with oxidative stress.

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