New Atmosphere, New Vision: Gibson and Whittemore Kick Off Invest in ME Conference 2016
Mark Berry reports on Dr. Gibson's introduction and Dr. Whittemore's keynote speech, at the 11th Invest in ME International ME Conference in London.
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Adrenergic Effect on Cytokine Release After Ex Vivo Healthy Volunteers’ Whole Blood LPS Stimulation

Discussion in 'Other Health News and Research' started by JaimeS, Apr 9, 2016.

  1. JaimeS

    JaimeS Senior Member

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    Adrenergic Effect on Cytokine Release After Ex Vivo Healthy Volunteers’ Whole Blood LPS Stimulation
    • Vasiliki Papandreou
    • , Nadia Kavrochorianou
    • , Theodoros Katsoulas
    • , Pavlos Myrianthefs
    • , Kyriaki Venetsanou
    • , George Baltopoulos
    Abstract
    Catecholamines are molecules with immunomodulatory properties in health and disease. Several studies showed the effect of catecholamines when administered to restore hemodynamic stability in septic patients. This study investigates the effect of norepinephrine and dobutamine on whole blood cytokine release after ex vivo lipopolysaccharide (LPS) stimulation. Whole blood collected from healthy individuals was stimulated with LPS, in the presence of norepinephrine or dobutamine at different concentrations, with or without metoprolol, a β1 receptor antagonist. Cytokine measurement was performed in isolated cell culture supernatants with ELISA. Results are expressed as mean ± SEM and compared with Mann-Whitney rank-sum test. Both norepinephrine and dobutamine significantly reduced TNF-α and IL-6 production after ex vivo LPS stimulation of whole blood in a dose-dependent manner, and this effect was partially reversed by the presence of metoprolol. Norepinephrine and dobutamine reduce the LPS-induced production of pro-inflammatory cytokines, thus possibly contributing to altered balance between the inflammatory and anti-inflammatory responses, which are vital for a successful host response to severe disease, shock, and sepsis.

    KEY WORDS
    sepsis inflammation norepinephrine dobutamine
    Rest of article is available on sci-hub.

    -J
     
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  2. shannah

    shannah Senior Member

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  3. Bob

    Bob

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  4. JaimeS

    JaimeS Senior Member

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    Good point, @Bob ! It doesn't mention ME/CFS by name -- I just checked. I posted it here because my Google Alert informed me of this article, and it's cued to inform me when someone cites certain ME/CFS researchers.

    Of course, once can use an ME/CFS study without discussing ME/CFS... and one can be an ME/CFS researcher and still write about other things. ;)

    -J

    [Edit: asked mods to move it.]
     
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  5. Justin30

    Justin30 Senior Member

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    I thought LPS has been moderately correlated to ME/CFS so wouldnt this paper be relveant even though it does not directly have ME/CFS in the title or the study?
     
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  6. JaimeS

    JaimeS Senior Member

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    Absolutely it's relevant... but it's not a CFS study. Hence its recategorization. :)

    -J
     
  7. Justin30

    Justin30 Senior Member

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    Fair enough. Thanks for posting it!
     
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