Hi, I have just finished reading through the thread 'B12: The Hidden Story' and I feel I am ready to start on the Active B12 protocol and I am about to get ordering my supplements. I am finding it quite hard to work out which order to do things in and how to work out starting dosages (this could well be due to my brain fog!) There seems to be a lot of different advice being given as the thread evolves over the years and I am just wondering what the most up to date protocol is? Is the 'Minimized One Month Trial to Detect Active Coblamin Responsiveness - Estimated 80% Detection Trial' which gives a week by week plan of what to do, still valid? http://forums.phoenixrising.me/index.php?threads/b-12-the-hidden-story.142/page-3#post-2619 I would find this straight forward to follow if it is still up to date. My other questions are: 1) What should I start with first (apart from the basic critical vitamins and minerals?) The enzymatic B12 infusion, the methyl B, the metfolin or the adb12? This is the different advice I have found over the course of the thread: A) The 'Minimised One Month Trial to Detect Active Cobalamin Responsiveness' recommends starting Methyl B on day 1 B) "If one starts the adb12 a couple of days before the mb12 then it separates to some degree the effects of each. Get going on 1 adb12 daily and work up the mb12 dosage" C) "Metafolin daily, Jarrow mb12 5mg daily, the Ezymatic Therapy 1mg can wait until you see if you need a little more help" D) "A single 1mg Enzymatic Therapy per day is quite adequate for most preople to start with. After a week, add dibencozide at least once a week. The Jarrow 5mg comes later as you step up the dose and when you go for maximum effectivness you will need both brands" 2) What is the best way to start the methyl B? The 'Minimized One Month Trial to Detect Active Coblamin Responsiveness' protocol gives the impression of recommending 1 x 5000 mcg methylB lozenge per day (if I have understood it correctly) whereas I have also seen these options posted: A) "Jarrow methyl B-12, 5mg, working up to 25mg a day (sublingual) I would suggest starting with 1 x 1000mcg Jarrow sublingual a day for 45-120+ minutes per day, increasing to 5 or 6 per day one at a time and then go to two at a time, then 3 at a time several times a day and then switch to the 5mg and increase by one at a time. Do them serially at first then increase dose size until an additional tablet makes no additional difference." B) "A 1000mcg tablet is a good place to start. To start out, 1/4 4 times in a day will probably work out better giving a more even serum level than taklng the whole 1mg at once. After a few day if you are not having too much start up responses you might start increasing by another quarter each day, going to half" C) "Jarrow Formulas Methyl-B12 5000 mcg: most correspondents take 2-4 spaced across the day; however, I'd suggest starting on one per day, and gradually working up" D) I also saw the 'Active B12 Titration Methods' post at the bottom of this thread: http://forums.bettermedicine.com/showthread.php/62327-active-b12-basics - is this the best advice to follow when titrating doses? 3) When is metfolin added in? The 'Minimized One Month Trial to Detect Active Cobalamin Responsiveness' recommends starting metfolin on day 1, however I have also found this advice: "When you are up to 3mg (methylB) daily add 1 Metafolin (Solgar methylfolate or other Metafolin brand" 4) How often per day is metfolin taken if one is starting out on 800 mcg? The 'Minimized One Month Trial to Detect Active Cobalamin Responsiveness' recommends taking it once per day, however after reading the advice below, does this mean it is necessary to split the 800 mcg dose in two? "B-complex and methylfolate need to be taken twice a day because of short serum half lifes for best effectiveness" I also read this post which might be answering some of my questions for me - if anyone thinks THIS is the most up to date way to go, then please let me know! "If a person is having a lot of startup reaction to mb12 then I would suggest starting with adb12. Either should be started on a base of the basic vitamins and minerals; A, D, C, E, B-complex that includes P-5-P and pantethine and without Cyanob12 (Jarrow B-Right, twice a day), methylfolate, magnesium, calcium, zinc (50mg) and Omega3 oils." Many, many thanks in advance for any advice anyone can give me!