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Hunting down the cause of ME/CFS & other challenging disorders - Lipkin in London
In a talk to patients in London on 3rd September, Dr. W. Ian Lipkin described the extraordinary lengths he and his team are prepared to go to in order to track down the source of an illness, with examples ranging from autism to the strange case of Kawasaki disease.
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A Natural Human Retrovirus Efficiently Complements Vectors Based on MuLV

Discussion in 'XMRV Research and Replication Studies' started by subtr4ct, May 27, 2010.

  1. subtr4ct

    subtr4ct Senior Member

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    Freely available: Link.

    Beihua Dong1, Robert H. Silverman1, Eugene S. Kandel2*
    1 Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America, 2 Department of Molecular Genetics and Virology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of Americ

    Abstract:

    Background
    Murine Leukemia Virus (MLV) is a rodent gammaretrovirus that serves as the backbone for common gene delivery tools designed for experimental and therapeutic applications. Recently, an infectious gammaretrovirus designated XMRV has been identified in prostate cancer patients. The similarity between the MLV and XMRV genomes suggests a possibility that the two viruses may interact when present in the same cell.

    Methodology/Principal Findings
    We tested the ability of XMRV to complement replication-deficient MLV vectors upon co-infection of cultured human cells. We observed that XMRV can facilitate the spread of these vectors from infected to uninfected cells. This functional complementation occurred without any gross rearrangements in the vector structure, and the co-infected cells produced as many as 104 infectious vector particles per milliliter of culture medium.

    Conclusions/Significance
    The possibility of encountering a helper virus when delivering MLV-based vectors to human cells in vitro and in vivo needs to be considered to ensure the safety of such procedures.
     

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