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7 years gone in vain.... 23andme Results help ??

Discussion in 'Genetic Testing and SNPs' started by ramkishore, Jun 23, 2014.

  1. ramkishore

    ramkishore

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    Hi all,


    Firstly thanks for reading this post.


    I was introduced to computer in 2006 July and from then my life has been a havoc til date. I can't think clearly at all. Extremely confused. I don't know what I speak. I cannot make eye contact for even 2 seconds. I think I suffer from Autistic tendency only after seeing my 23andme results as described below.


    Adding further to this injury , I had mercury filling introduced in 2009 July and removed it in 2013 Dec. Almost 4 years mercury poisoned.The hair analysis test showed mercury, High Al, Lead and good amount of Arsenic too.


    Till date I don't feel connected to myself, or to this earth.


    I got my 23andme results just a week ago and here are my results.


    COMT V158M -/-
    COMT H62H +/-
    COMT P199P -/-

    VDR Bsm +/-
    VDR Taq +/+

    MAO A R297R +/+

    ACAT1-02 -/-


    MTHFR C677T -/-
    MTHFR 03 P39P -/-
    MTHFR A1298C +/-

    MTR A2756G +/-
    MTRR A66G +/+
    MTRR H595Y n/a
    MTRR K350A -/-
    MTRR R415T n/a
    MTRR A664A +/-

    BHMT-02 -/-
    BHMT-04 n/a
    BHMT-08 -/-


    AHCY-01 +/-
    AHCY-02 n/a
    AHCY-19 +/-


    CBS C699T -/-
    CBS A360A +/-
    CBS N212N n/a

    SHMT1 C1420T n/a


    It is becoming increasingly difficult for me to understand where the problem is and How to address it especially after reading Amy Yasko book as the first step of finding SHMT1 is not found in the test I have done and recursively same happening with CBS N212N n/a,AHCY-02 n/a which could downregulate the upregulation of CBS ,BHMT-04 n/.

    How should I go about interpreting these results?

    I heard about G6DP can be found out from raw data at 23andme.com but don't know which SNP to look out for? could somebody help me here.

    My experience:
    1. I cannot tolerate any DMPS/DMSA which I did earlier. I even tried with Chlorella and Cilantro but it was not of any use. I concluded this as I did not find any change with regards to autistic symptoms even after 2 months of continuous usage.
    2. my nerves shiver with excess protein as its clear the next day I eat chicken.
    3. right in the center of brain i feel a motion always. I think it could be mercury which is trapped in CNS as will be the case for B+ blood group where mercury enters nervous system.
    4. Damn Damn..I am always in fight/flight mode. I cannot feel peace in the body itself.
    5. On mineral test I showed high Na, K , Ca and Mg. I was strange for me to note all those were in very high region.


    How should I go about NOS, SUOX, ACE testing which are not done in 23andme.Can I carry the treatment with these inputs.I never had any serious gut issues from childhood other than constipation. So I assume good gut health.


    Eagerly awaiting your inputs

    Warm Regards of Kishore
    victoriana likes this.
  2. Daffodil

    Daffodil Senior Member

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    constipation can indicate serious gut issues despite no other symptoms

    is there a way for you to see a cfs specialist?
    ramkishore likes this.
  3. Valentijn

    Valentijn Activity Level: 3

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    First I'd put down the Yasko book. Most of her claims are not based on published research, and some are even contradicted by it.

    MTRR A66G means that MTR is being recycled at about 30% of the usual rate. Supplementing B12 might therefore be helpful in speeding up that recycling. Some people tolerate hydroxoB12 better than methylB12.
  4. Lillybelle

    Lillybelle

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    Hi Valentijn, thats interesting.Who's research regarding methylation,genetic testing and treatment would be better to review. It seems that all roads lead back to Yasko regarding methylation treatment based on various gentic SNP's.Rich Van Konynenburg's protocal refers to Yasko's work as his major source and its clear they collaborated. As is Dr Sarah Myhill's treatment approach based on Rich's work. Rich only ran one published trial of 30 patients on methylation I now of. However I dont think the treatment was based on the genetic testing.

    Any sources except these I would love to read if you have access to them. The only other source I've found is the Dr. who runs MTHFR.net.
    Thanks for yr help as Im currently trying methylation and was about to read Yasko's book.:)
    victoriana likes this.
  5. Valentijn

    Valentijn Activity Level: 3

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    I'd suggest reading the research published by the actual researchers. Yasko is not an MD and is highly invested in selling expensive products, in addition to being proven very wrong about some of her claims.

    Google scholar is a good place to look up real research involving SNPs, and there are also often links to research at http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs= and http://snpedia.com/index.php/SNPedia
    TheGrandWazoo and victoriana like this.
  6. Lillybelle

    Lillybelle

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    Do you have any suggestions on which researchers. It would be good to get some names of these published researchers? Also Dr. Myhill bases her plan on Rich's plan which is based on Yasko's work. So some MD's are using her work.
  7. Lillybelle

    Lillybelle

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    Thanks will give it a look
  8. Lillybelle

    Lillybelle

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    upload_2014-6-23_21-43-18.png
    This is what I found from yr source. Sorry It doesnt look like published research I could understand. Perhaps Im using the wrong search terms.
    Any direction here or well known researchers in this area would be most appreciated.
    upload_2014-6-23_21-43-18.png
  9. Lillybelle

    Lillybelle

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    Same Problem with SNP. No search resulys for CFS/M.E. or CFS/ME research. upload_2014-6-23_21-48-5.png
  10. Valentijn

    Valentijn Activity Level: 3

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    You need to search for the SNP you're interested in.
    victoriana likes this.
  11. Lillybelle

    Lillybelle

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    Yeah Im trying to find published Research though for methylation on cfs based on genetic testing. So if you know some other well published researchers that would be really helpful for the search. Thanks!
  12. ramkishore

    ramkishore

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    The whole problem is not finding a CFS specialist here in India.They will refer me to phsyciatrist if I speak of genetic nutrition.

    If I manage to drink more than 1 litre of water/day( excluding the one you drink while having food), I will not have constipation..i mean free bowl movement next morning. But if I dont drink then I will not have bowl movement for more than 2 days or even more. You still see gut issue with water included in the picture?

    you know how to look up for G6DP?

    Awaiting your reply

    Thanks in Advance.

    Warm Regards
  13. Daffodil

    Daffodil Senior Member

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    hi ramkishore. you mentioned that your problems began when you started using a computer. I am not sure I understand...how can using a computer cause this?

    would it be possible for you to travel outside of india?

    if not, I believe there may be doctors in india who can help. there is a pediatrician, Dr Bariah Dardari, who deals with autism and uses GcMAF, one of the treatments used for CFS by some specialists in north America. perhaps this doctor can refer you to someone in india.

    dr. chitra bhakta is a specialist in California.. a very helpful lady. if you contact her office, they may know of someone in india who can help.

    in the meantime, you could perhaps try one of the diets for autoimmune disease.

    I do not know much about the genetic mutations, sorry I cannot help with that.

    my CFS specialist has recommended I drink 2 L of water a day.

    maybe high quality probiotics might help?
    Last edited: Jun 23, 2014
    victoriana likes this.
  14. ramkishore

    ramkishore

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    Computer emits EMF which can cause autism. I am electro chemical sensitive. Just sitting besides WiFi point I can start getting headaches.

    Currently I am unable to come over there. Financial restrictions.
    I will surely checkout those doctors. many thanks for that.


    do you still think I have gut issues even after removing constipation with water?

    Thanks
  15. caledonia

    caledonia

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    Do you mean you are experiencing problems from EMFs (electro magnetic frequencys)? You may have to limit your time on the computer, or you can possibly shield your computer from emitting EMFs.

    These toxic metals can cause a wide range of negative effects.

    These genes actually aren't too bad. The worst ones are MTR + MTRR, which is the "B12 double whammy". Some B12 supplementation would be suggested for that. Your COMT/VDR combination is the least sensitive, so it should be fine to take all three types of B12 - hydroxycobalamin, methylcobalamin and adenosylcobalamin. Avoid cyanocobalamin, unless that's your only choice.

    You would also want to take some methylfolate for the MTHFR mutation. Lead and mercury can also cause problems with MTHFR and MTR, so again folate and B12 would be suggested.

    CBS A360A is the minor one. Usually people with this one don't need to worry about CBS. There is also an absence of BHMT mutations which can add to CBS problems. So it's unlikely that you'll need to treat for CBS.

    The new v chip unfortunately doesn't show SHMT anymore. The treatment for that is simply folinic acid. You can try taking it anyway and see if you tolerate it (most people should be ok with it).

    Here is info regarding the rs number - you need to know this to find it in the raw data in 23andme.
    http://www.snpedia.com/index.php/Rs1050829
    http://www.snpedia.com/index.php/Rs5030868


    My experience:
    This is not surprising if you're not detoxing well. At any rate, two months is not nearly long enough to get metals detoxed.
    I'm not sure what's going on here, except for chicken can have arsenic (from their feed). You can try finding an organic source of chicken to see if you tolerate that better. If you're still not tolerating chicken, then perhaps you have a food allergy or intolerance. That would suggest having leaky gut.
    If this is the case, then you could also have leaky gut and would want to treat for that first.

    My guess would be you have high glutamate and low GABA. Treatment would be to avoid foods with MSG and high glutamates and to supplement with GABA. Vitamin B6 and zinc can also help your body convert glutamate to GABA. Take the active form of B6 called P5P.

    MSG is in most processed foods, so to make it simple, avoid processed foods and seasonings. Then also avoid tomatoes, peas, Parmesan cheese and mushrooms, which naturally have high glutamates.
    This is unusual. Usually we are low in electrolytes. It's possible that the test was showing what's in the blood, but not in the cells. In that case, you could actually be deficient. What kind of test did you do? Do you have any deficiency symptoms such as craving salty foods, heart palpitations, muscle cramps, twitchy feet waking you up during sleep?

    Have you done any adrenal testing, such as the 24 hour saliva test? The adrenals help regulate electrolyte levels. People with weak adrenals will be leaking out electrolytes like crazy.


    Don't worry about these.

    Constipation could indicate low electrolytes or low probiotics. The high toxic metals also suggest bad gut bugs holding onto metals. I would suggest getting a stool test to see what's really going on. See the link in my signature for the 4R Gut Rebuilding Program.

    Can you also post your Detox panel results? (available from geneticgenie.org).

    Tag me with @caledonia so I can get an alert that you have posted.
  16. Daffodil

    Daffodil Senior Member

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    ramkishore...I don't think absence of gut symptoms indicates absence of gut issues. there are stool tests you can take, but they have many shortcomings as this is a relatively new science.

    I am sure you could find a suitable doctor somewhere closer to you, though it might take some searching. this would be my first priority.

    best of luck!
  17. ramkishore

    ramkishore

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    @caledonia

    Many thanks Caledonia for the reply. It gave me so much relief.

    I have taken care of this EMF from computer by using screen filter now.

    My only hunt is for the answer to " why is my body not detoxing?"

    In my effort to detox, when ever I eat cruicferous veg like cabbage, broccoli etc or take DMPS, DMSA I feel worst. So I thought I have problem with sulphur digestion.I did take gluthathione reduced too but did not feel good at all. So the problem could be with SUOX, G6DP not working correctly which work on sulphites and make them harmless or CBS upregulation( even CBS A360A could cause this as said by Yasko) which generates more Taurine, sulphites and thus less gluthathione.Thats why I am not detoxing properly because of low gluthathione.

    Here are my G6DP results from 23andme.com. How do I interpret them with +/-?

    G6DP rs5030868 A or G G
    G6DP Rs1050829 C or T T

    The other answer for my body not detoxing properly according to you could be poor gut function. I think only metametrix can reveal this. what is the cost for that single test? I think I should get that to India. I am not sure if they do international shipping.kindly let me know the cost.

    Regarding minerals, it was hair mineral analysis test. i was damn low in molybdenum, low in manganese too. I will take adrenal test you mentioned.

    It would take me lot of time to absorb the information you have written.I will come back with questions if you don't mind.

    Here is my detox profile results. thanks for your interest in this.
    CYP1A1*2C A4889G +/-
    CYP1A1 m3 T3205C -/-
    CYP1A1 C2453A -/-
    CYP1A2 164A>C +/-
    CYP1B1 L432V +/-
    CYP1B1 N453S -/-
    CYP1B1 R48G +/-
    CYP2A6*2 1799T>A -/-
    CYP2A6*20 -/-
    CYP2C9*2 C430T -/-
    CYP2C9*3 A1075C -/-
    CYP2C19*17 -/-
    CYP2D6 S486T +/-
    CYP2D6 100C>T -/-
    CYP2D6 2850C>T +/-
    CYP2E1*1B 9896C>G -/-
    CYP2E1*1B 10023G>A -/-
    CYP2E1*4 4768G>A -/-
    CYP3A4*1B -/-
    CYP3A4*2 S222P -/-
    CYP3A4*3 M445T -/-
    CYP3A4*16 T185S -/-
    GSTP1 I105V +/-
    GSTP1 A114V -/-
    SOD2 A16V -/-
    NAT1 R187Q -/-
    NAT1 R64W -/-
    NAT2 I114T -/-
    NAT2 R197Q -/-
    NAT2 G286E -/-
    NAT2 R64Q -/-
    NAT2 K268R -/-


    The only answer i want is " why is my body unable to detox at all?"

    take home conclusion: take metametrix CSA test to find out the gut issues said by caledonia.

    Thanks for shifting the gears.
    Last edited: Jun 23, 2014
  18. caledonia

    caledonia

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    You're welcome.

    Good.

    My only hunt is for the answer to " why is my body not detoxing?"

    That is starting to sound like a CBS or SUOX problem. Plus you're low on molybdenum which helps to detox sulfur.

    You have to find out which one is the risk allele. There are two strands, one from each parent. 23andme reports one strand, while studies often report the other strand. So you have to convert between them. I can never remember the conversion off the top of my head, or find a chart for it.

    It should be accurate. You might want to start supplementing with those. Keep moly to about 75mcg. You don't want to drive sulfur too low.

    If your adrenals turn out to be high, that could make you feel stressed and anxious. You can take Relora or Seriphos for 4-8 weeks to get that calmed down.

    CYP1A2 is a slow caffeine metabolizer.
    CYP1B1 can lead to estrogen dominance and possibly estrogen related cancers - breast, cervical, etc. for females and prostate for males. You can eat cruciferous veggies or take DIM or IC3 if your estrogen is high. If you have problems with CBS or sulfur, then you can take calcium d glucarate.
    CYP2D6 detoxifies 20% of all prescription drugs including psych drugs and beta blockers. You could have problems with these or require smaller doses.

    GSTP affects glutathione.

    MTR + MTRR + MTHFR A1298C + GSTP make you have less ability to produce glutathione. This causes a vicious circle where metals can accumulate. The accumulation of metals further causes reduction in methylation, which causes further reduction in glutathione etc. etc. etc.


    =-==-=-=-
    So I would suggest:

    1) A stool test to see how your gut is functioning, and then if there are any problems there, do the 4R Gut Rebuilding Program. This will start to detox metals. This will take several months.

    2) a CBS treatment (see the Heartfixer page for the best info). You do the same treatment whether you have CBS or SUOX. This will take several more months.

    3) then start adding in B12 and folate which will restart methylation and make more metals come out. There are many co-factors that go with those, plus add supplementation for anything you're deficient in. The metals may make you feel worse as they come out, so going Low and Slow is key so you can tolerate treatment.

    4) if any metals are remaining you should be able to tolerate DMPS, etc. at this point.

    I'm not a doctor, just a well researched patient working through this herself, so it would be good to have a doctor on board. Check the Find a Practitioner link in my signature. There are several practitioners who will work via phone and Skype and also work internationally.

    Also please read "Start Low and Go Slow" and "Roadblocks to Successful Methylation" before beginning (also in my signature). I have more detailed info on all of this stuff there.
    Alea Ishikawa and ramkishore like this.
  19. ramkishore

    ramkishore

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    @caledonia

    Thanks for creating the big picture. I am zeroing in on to the source of problem.

    Which book you read for understanding those genes from Detox Profile?

    You recommend B12 dosing first for treating MTR+MTRR.Then go to folate dosing for MTHFR1298C.?? Or start both at the same time.

    CBS causes less gluthathione and more taurine when cysteine gets high. What causes high cysteine?

    CBS produces two harmful substances : ammonia and sulphites.
    How handling ammonia and sulphites will down regulate CBS? Some catch is here which I am missing.

    I enquired about Metametrix GI CSA test here and came to know there is no such test being done here. very sad.

    I was told about another stool analysis which will detect the pathogens. will that not be sufficient to go ahead in the program?

    eagerly awaiting your response.

    your replies have helped me a lot

    Thanks in advance.
  20. caledonia

    caledonia

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    I use a combination of the Detoxigenomics report and the MTHFRsupport podcast on the SNPs, which are both linked in my signature. You can also look up individual SNPs in SNPedia. That helped me figure out the caffeine one.

    The latest from Ben Lynch is to start both at the same time, but I think I would try them separately for a few days to test for tolerance.

    This is my simplified understanding of it - CBS is an upregulation instead of the usual downregulation we think of with gene mutations. Ammonia and sulfur go shooting down the CBS pathway and get clogged up at SUOX, which has trouble detoxing them. This causes any methyl groups that come through CBS to get shunted into creating inflammation, instead of creating glutathione.

    So the treatment is to lower ammonia and sulfur to get the load down, and also help SUOX detox with molybdenum to get the pathway operating properly again. Then you should be able to tolerate sulfur, ammonia, and methyl groups and start making glutathione.

    It's been proven that some details of what Yasko claims about this are incorrect, but the basic facts still hold true - at least it all worked out for me that way.

    If the other stool test I mention in the 4R Gut Rebuilding document is available, that would be fine (can't think of the name of it off the top of my head). Or if that's not available, the nurse at Integrative Psychiatry may be able to suggest something for you. http://www.integrativepsychiatry.net/
    victoriana likes this.

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