Dr. Marc-Alexander Fluks posted this to co-cure 2010 01 14
Source: University of California, Los Angeles
Date: 2007
URL: http://gradworks.umi.com/32/80/3280930.html
http://proquest.umi.com/pqdlink?did=1414115021&Fmt=7&clientI d=79356&RQT=309&VName=PQD
Rem: Ph.D. Thesis, 138 pages; ISBN: 9780549234111
Retrovirus integration site selection and its biological significance
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by Kim, Sanggu, PhD
Abstract (...)
We have conducted integration site analysis for a recently identified Xenotropic murine leukemia virus-related virus (XMRV). XMRV is a previously undescribed gammaretrovirus that infects human cells. A strong correlation of XMRV infection with prostate cancer formation in patients who are homozygous for a RNase L variant has been shown.
In this study, 472 XMRV integration sites in a human prostate cell line DU 145 were sequenced and compared to other retroviruses. Integration site selection of XMRV shares a largely conserved pattern with that of MLV and FV, but it also shows significantly higher preference for transcription start sites compared to the other retroviruses. The result suggests that XMRV integration into the genome may have strong genotoxicity for the host cell.
Viruses have long been studied for the association with cancer. Recent identification of XMRV infection in prostate tissue from
patients with variants in RNASEL, an innate antiviral agent and also one of hereditary prostate cancer genes, suggested a viral etiology in these patients. In this study, 16 XMRV integration sites in prostate cancer tissues from 8 patients who are homozygous for the RNASEL variant R462Q were sequenced and analyzed. The chromosomal regions hosting the 16 integration sites have a high correlation with prostate cancer development. Gene ontology study and cancer database search for the genes nearest to the 16 sites also showed a strong correlation with mechanisms involved in cancer formation.
For comparison, genes near XMRV integration sites in acute infection and human cancer genes in Affymetrix G110 array were used. The results suggest a potential causative role of XMRV integration in prostate cancer formation.
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(c) 2007 University of California, Los Angeles
Source: University of California, Los Angeles
Date: 2007
URL: http://gradworks.umi.com/32/80/3280930.html
http://proquest.umi.com/pqdlink?did=1414115021&Fmt=7&clientI d=79356&RQT=309&VName=PQD
Rem: Ph.D. Thesis, 138 pages; ISBN: 9780549234111
Retrovirus integration site selection and its biological significance
---------------------------------------------------------------------
by Kim, Sanggu, PhD
Abstract (...)
We have conducted integration site analysis for a recently identified Xenotropic murine leukemia virus-related virus (XMRV). XMRV is a previously undescribed gammaretrovirus that infects human cells. A strong correlation of XMRV infection with prostate cancer formation in patients who are homozygous for a RNase L variant has been shown.
In this study, 472 XMRV integration sites in a human prostate cell line DU 145 were sequenced and compared to other retroviruses. Integration site selection of XMRV shares a largely conserved pattern with that of MLV and FV, but it also shows significantly higher preference for transcription start sites compared to the other retroviruses. The result suggests that XMRV integration into the genome may have strong genotoxicity for the host cell.
Viruses have long been studied for the association with cancer. Recent identification of XMRV infection in prostate tissue from
patients with variants in RNASEL, an innate antiviral agent and also one of hereditary prostate cancer genes, suggested a viral etiology in these patients. In this study, 16 XMRV integration sites in prostate cancer tissues from 8 patients who are homozygous for the RNASEL variant R462Q were sequenced and analyzed. The chromosomal regions hosting the 16 integration sites have a high correlation with prostate cancer development. Gene ontology study and cancer database search for the genes nearest to the 16 sites also showed a strong correlation with mechanisms involved in cancer formation.
For comparison, genes near XMRV integration sites in acute infection and human cancer genes in Affymetrix G110 array were used. The results suggest a potential causative role of XMRV integration in prostate cancer formation.
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(c) 2007 University of California, Los Angeles