MeSci
ME/CFS since 1995; activity level 6?
- Messages
- 8,231
- Location
- Cornwall, UK
Predictable...?
Date: August 5, 2017
URL: http://onlinelibrary.wiley.com/doi/10.1111/cei.13023/abstract
Cytokine responses to exercise and activity in patients with
chronic fatigue syndrome: Case control study
----------------------------------------------------------
L.V. Clark(1), M. Buckland(2,*), G. Murphy(2), N. Taylor(2),
V. Vleck(3), C. Mein(4), E. Wozniak(4), M. Smuk(1), P.D. White(1)
1 Centre for Psychiatry, Wolfson Institute of Preventive Medicine,
Queen Mary's School of Medicine and Dentistry, London, EC1M 6BQ.
2 UCL Centre for Immunodeficiency, Royal Free London NHS
Foundation Trust, London, WC1E 6BT.
3 CIPER, Faculty of Human Kinetics, University of Lisbon, Estrada
da Costa, Cruz Quebrada- Dafundo, 1499-002 Lisbon, Portugal.
4 Genome Centre, Barts and the London School of Medicine and
Dentistry, Queen Mary University of London, Charterhouse Square,
London EC1M 6BQ.
* Corresponding author: Dr Matthew Buckland.
E-mail: mbuckland@nhs.net
Summary
Chronic fatigue syndrome (CFS) is characterized by fatigue after
exertion. A systematic review suggested that transforming growth factor
beta (TGF-beta) concentrations are often elevated in cases of CFS when
compared to healthy controls. This study attempted to replicate this
finding, and investigate whether post-exertional symptoms were
associated with altered cytokine protein concentrations and their RNA in
CFS patients.
Twenty-four patients fulfilling Centers for Disease Control criteria for
CFS, but with no comorbid psychiatric disorders, were recruited from two
CFS clinics in London, UK. Twenty-one healthy, sedentary controls were
matched by gender, age, and other variables. Circulating proteins and
RNA were measured for TGF-beta, TNF, IL-8, IL-6 and IL-1beta. We
measured six further cytokine protein concentrations (IL-2, IL-4, IL-5,
IL-10, IL-12p70, and IFN-gamma). Measures were taken at rest, and before
and after both commuting and aerobic exercise.
CFS cases had higher TGF-beta protein levels compared to controls at
rest (median (quartiles) = 43.9 (19.2, 61.8) versus 18.9 (16.1, 30.0)
ng/ml) (p = 0.003), and consistently so over a nine-day period. However,
this was a spurious finding due to variation between different assay
batches.
There were no differences between groups in changes to TGF-beta protein
concentrations after either commuting or exercise. All other cytokine
protein and RNA levels were similar between cases and controls.
Post-exertional symptoms and perceived effort were not associated with
any increased cytokines.
We were unable to replicate previously found elevations in circulating
cytokine concentrations, suggesting that elevated circulating cytokines
are not important in the pathophysiology of CFS.
--------
(c) 2017 John Wiley & Sons, Inc.
Date: August 5, 2017
URL: http://onlinelibrary.wiley.com/doi/10.1111/cei.13023/abstract
Cytokine responses to exercise and activity in patients with
chronic fatigue syndrome: Case control study
----------------------------------------------------------
L.V. Clark(1), M. Buckland(2,*), G. Murphy(2), N. Taylor(2),
V. Vleck(3), C. Mein(4), E. Wozniak(4), M. Smuk(1), P.D. White(1)
1 Centre for Psychiatry, Wolfson Institute of Preventive Medicine,
Queen Mary's School of Medicine and Dentistry, London, EC1M 6BQ.
2 UCL Centre for Immunodeficiency, Royal Free London NHS
Foundation Trust, London, WC1E 6BT.
3 CIPER, Faculty of Human Kinetics, University of Lisbon, Estrada
da Costa, Cruz Quebrada- Dafundo, 1499-002 Lisbon, Portugal.
4 Genome Centre, Barts and the London School of Medicine and
Dentistry, Queen Mary University of London, Charterhouse Square,
London EC1M 6BQ.
* Corresponding author: Dr Matthew Buckland.
E-mail: mbuckland@nhs.net
Summary
Chronic fatigue syndrome (CFS) is characterized by fatigue after
exertion. A systematic review suggested that transforming growth factor
beta (TGF-beta) concentrations are often elevated in cases of CFS when
compared to healthy controls. This study attempted to replicate this
finding, and investigate whether post-exertional symptoms were
associated with altered cytokine protein concentrations and their RNA in
CFS patients.
Twenty-four patients fulfilling Centers for Disease Control criteria for
CFS, but with no comorbid psychiatric disorders, were recruited from two
CFS clinics in London, UK. Twenty-one healthy, sedentary controls were
matched by gender, age, and other variables. Circulating proteins and
RNA were measured for TGF-beta, TNF, IL-8, IL-6 and IL-1beta. We
measured six further cytokine protein concentrations (IL-2, IL-4, IL-5,
IL-10, IL-12p70, and IFN-gamma). Measures were taken at rest, and before
and after both commuting and aerobic exercise.
CFS cases had higher TGF-beta protein levels compared to controls at
rest (median (quartiles) = 43.9 (19.2, 61.8) versus 18.9 (16.1, 30.0)
ng/ml) (p = 0.003), and consistently so over a nine-day period. However,
this was a spurious finding due to variation between different assay
batches.
There were no differences between groups in changes to TGF-beta protein
concentrations after either commuting or exercise. All other cytokine
protein and RNA levels were similar between cases and controls.
Post-exertional symptoms and perceived effort were not associated with
any increased cytokines.
We were unable to replicate previously found elevations in circulating
cytokine concentrations, suggesting that elevated circulating cytokines
are not important in the pathophysiology of CFS.
--------
(c) 2017 John Wiley & Sons, Inc.